Label-Free Carbon-Dots-Based Ratiometric Fluorescence pH Nanoprobes for Intracellular pH Sensing

2016 ◽  
Vol 88 (15) ◽  
pp. 7837-7843 ◽  
Author(s):  
Jingfang Shangguan ◽  
Dinggeng He ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Fengzhou Xu ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Carbon Dots ◽  
Free Carbon ◽  
Ph Sensing ◽  
Label Free ◽  
Ratiometric Fluorescence

A facile synthesis of label-free carbon dots with unique selectivity-tunable characteristics for ferric ion detection and cellular imaging applications

2019 ◽  
Vol 43 (12) ◽  
pp. 4734-4744 ◽  
Author(s):  
Kok Ken Chan ◽  
Chengbin Yang ◽  
Yi-Hsin Chien ◽  
Nishtha Panwar ◽  
Ken-Tye Yong
Keyword(s):  
Carbon Dots ◽  
Metal Ion ◽  
Cellular Imaging ◽  
Free Carbon ◽  
Ferric Ion ◽  
Label Free ◽  
Ion Detection ◽  
Facile Synthesis ◽  
Ferric Ion Detection

The application of a pH-tuning concept to create specific analytical responses of carbon dots towards a specific targeted metal ion.

scholarly journals Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 604 ◽  
Author(s):  
Maojia Huang ◽  
Xinyue Liang ◽  
Zixiao Zhang ◽  
Jing Wang ◽  
Yiyan Fei ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Living Cells ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Gradual Change ◽  
Ph Sensing ◽  
Lifetime Imaging

The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6–8.6. Similar fluorescence lifetime changes were found in pH buffer-treated living cells. The detection of lysosomes, cytoplasm, and nuclei in living cells was achieved by measuring the fluorescence lifetime of CDs. In particular, a phasor FLIM analysis was used to improve the pH imaging. Moreover, the effects of the coenzymes, amino acids, and proteins on the fluorescence lifetime of CDs were examined in order to mimic the complex microenvironment inside the cells.

Boron Doped Carbon Dots with Unusually High Photoluminescence Quantum Yield for Ratiometric Intracellular pH Sensing

ChemPhysChem ◽  
2019 ◽  
Vol 20 (8) ◽  
pp. 1018-1027 ◽  
Author(s):  
Ayan Pal ◽  
Kafeel Ahmad ◽  
Deepanjalee Dutta ◽  
Arun Chattopadhyay
Keyword(s):  
Quantum Yield ◽  
Intracellular Ph ◽  
Carbon Dots ◽  
Ph Sensing ◽  
Boron Doped ◽  
Photoluminescence Quantum Yield ◽  
Doped Carbon Dots

Green preparation of carbon dots for intracellular pH sensing and multicolor live cell imaging

2016 ◽  
Vol 4 (44) ◽  
pp. 7130-7137 ◽  
Author(s):  
Wen-Jing Wang ◽  
Jun-Mei Xia ◽  
Ji Feng ◽  
Meng-Qi He ◽  
Ming-Li Chen ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Carbon Dots ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Emission Feature ◽  
Ph Sensing ◽  
Nitrogen Doped ◽  
Multicolor Imaging ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Nitrogen-doped carbon dots exhibit a distinct pH-sensitive/excitation-dependent photoluminescence emission feature within pH 4.0–8.0, facilitating intracellular pH sensing and multicolor imaging of live HeLa cells.

Highly sensitive Ni 2+ sensors based on polyurethane‐derived, label‐free carbon dots with high adsorption capacity

2021 ◽  
Vol 6 (31) ◽  
pp. 7964-7971
Author(s):  
Kulpriya Phetcharee ◽  
Natee Sirisit ◽  
Jedsada Manyam ◽  
Peerasak Paoprasert
Keyword(s):  
Adsorption Capacity ◽  
Carbon Dots ◽  
Free Carbon ◽  
Label Free ◽  
High Adsorption ◽  
Highly Sensitive ◽  
High Adsorption Capacity
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