scholarly journals Fast and Simple Protocols for Mass Spectrometry-Based Proteomics of Small Fresh Frozen Uterine Tissue Sections

2017 ◽  
Vol 89 (20) ◽  
pp. 10769-10775 ◽  
Author(s):  
Irena Dapic ◽  
Naomi Uwugiaren ◽  
Petra J. Jansen ◽  
Garry L. Corthals
Keyword(s):  
Mass Spectrometry ◽  
Uterine Tissue ◽  
Tissue Sections ◽  
Fresh Frozen

scholarly journals Exemplifying the Screening Power of Mass Spectrometry Imaging over Label-Based Technologies for Simultaneous Monitoring of Drug and Metabolite Distributions in Tissue Sections

2015 ◽  
Vol 21 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Richard J. A. Goodwin ◽  
Anna Nilsson ◽  
C. Logan Mackay ◽  
John G. Swales ◽  
Maria K. Johansson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Cyclic Peptide ◽  
Analysis Data ◽  
Resolving Power ◽  
High Mass ◽  
Whole Body ◽  
Ion Cyclotron Resonance ◽  
Label Free ◽  
Tissue Sections

Mass spectrometry imaging (MSI) provides pharmaceutical researchers with a suite of technologies to screen and assess compound distributions and relative abundances directly from tissue sections and offer insight into drug discovery–applicable queries such as blood-brain barrier access, tumor penetration/retention, and compound toxicity related to drug retention in specific organs/cell types. Label-free MSI offers advantages over label-based assays, such as quantitative whole-body autoradiography (QWBA), in the ability to simultaneously differentiate and monitor both drug and drug metabolites. Such discrimination is not possible by label-based assays if a drug metabolite still contains the radiolabel. Here, we present data exemplifying the advantages of MSI analysis. Data of the distribution of AZD2820, a therapeutic cyclic peptide, are related to corresponding QWBA data. Distribution of AZD2820 and two metabolites is achieved by MSI, which [14C]AZD2820 QWBA fails to differentiate. Furthermore, the high mass-resolving power of Fourier transform ion cyclotron resonance MS is used to separate closely associated ions.

New Developments in Profiling and Imaging of Proteins from Tissue Sections by MALDI Mass Spectrometry

2006 ◽  
Vol 5 (11) ◽  
pp. 2889-2900 ◽  
Author(s):  
Pierre Chaurand ◽  
Jeremy L. Norris ◽  
D. Shannon Cornett ◽  
James A. Mobley ◽  
Richard M. Caprioli
Keyword(s):  
Mass Spectrometry ◽  
Maldi Mass Spectrometry ◽  
New Developments ◽  
Tissue Sections

Mass spectrometry imaging of pharmacological compounds in tissue sections

Bioanalysis ◽  
2010 ◽  
Vol 2 (2) ◽  
pp. 279-293 ◽  
Author(s):  
Richard JA Goodwin ◽  
Andrew R Pitt
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Tissue Sections

scholarly journals MALDI TIMS IMS of Disialoganglioside Isomers – GD1a and GD1b in Murine Brain Tissue

2021 ◽  
Author(s):  
Katerina Djambazova ◽  
Martin Dufresne ◽  
Lukasz Migas ◽  
Angela Kruse ◽  
Raf Van de Plas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Sialic Acid ◽  
Gas Phase ◽  
Sialic Acid Residue ◽  
Spinal Cord Tissue ◽  
Total Ganglioside ◽  
Tissue Sections ◽  
Maldi Ims ◽  
Gas Phase Separation

Gangliosides are classified as acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or multiple sialic acid residue(s). The presence of multiple sialylation sites gives rise to highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility mass spectrometry (TIMS), a gas-phase separation technology, with MALDI IMS allows for the investi-gation of isomeric lipid structures in situ. Here we demonstrate the gas-phase separation of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in a standard mixture of both isomers, a total ganglioside extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) were deter-mined from rat hippocampus, as well as in a spinal cord tissue section.

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