scholarly journals Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry

2012 ◽  
Vol 403 (7) ◽  
pp. 1851-1861 ◽  
Author(s):  
Yuki Sugiura ◽  
Nobuhiro Zaima ◽  
Mitsutoshi Setou ◽  
Seiji Ito ◽  
Ikuko Yao
Keyword(s):  
Mass Spectrometry ◽  
Central Nervous System ◽  
Nervous System ◽  
Imaging Mass Spectrometry ◽  
Central Nervous System Tissue ◽  
Tissue Sections

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue

2001 ◽  
Vol 149 (19) ◽  
pp. 577-582 ◽  
Author(s):  
J. Grassi ◽  
S. Simon ◽  
C. Crminon ◽  
Y. Frobert ◽  
E. Comoy ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Rapid Test ◽  
Central Nervous System Tissue ◽  
Postmortem Diagnosis
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