A Doubly-Quenched Fluorescent Probe for Low-Background Detection of Mitochondrial H2O2

The design strategy of a bioprobe based on a D–A–D type fluorophore was discussed, and CBFB constructed by this kind of fluorophore showed specific biothiol recognition in living cells.

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A fluorescent probe with low-background for visualization of nitroxyl in living cells and zebrafish

Dyes and Pigments ◽

10.1016/j.dyepig.2020.108889 ◽

2021 ◽

Vol 185 ◽

pp. 108889

Author(s):

Changli Zhang ◽

Fengyun He ◽

Fang Huang ◽

Jian Xu ◽

Yaojuan Hu

Keyword(s):

Fluorescent Probe ◽

Living Cells ◽

Low Background

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A highly sensitive and selective fluorescence off–on probe for the detection of intracellular endogenous tyrosinase activity

Chemical Communications ◽

10.1039/c6cc09679d ◽

2017 ◽

Vol 53 (16) ◽

pp. 2443-2446 ◽

Cited By ~ 42

Author(s):

Xiaofeng Wu ◽

Xiaohua Li ◽

Hongyu Li ◽

Wen Shi ◽

Huimin Ma

Keyword(s):

Fluorescent Probe ◽

High Sensitivity ◽

Tyrosinase Activity ◽

Background Signal ◽

Low Background ◽

Highly Sensitive

A fluorescent probe with a low background signal and high sensitivity is developed and applied to image the relative levels of tyrosinase activity in different cells.

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Rational design of near-infrared fluorophores with phenolic D−A type structure and construction of a fluorescent probe for cysteine imaging

New Journal of Chemistry ◽

10.1039/d1nj02459k ◽

2021 ◽

Author(s):

Dugang Chen ◽

Gang Nie ◽

Yecheng Dang ◽

Wenjie Liang ◽

Wanqing Li ◽

...

Keyword(s):

Penetration Depth ◽

Fluorescent Probe ◽

Fluorescent Probes ◽

Rational Design ◽

Near Infrared ◽

Type Structure ◽

Deep Penetration ◽

Biological Applications ◽

Low Background ◽

Nir Emission

Fluorescent probes with near-infrared (NIR) emission have attracted great attentions in the biological applications because of the low background interference and deep penetration depth. Therefore, as an important component of...

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Direct detection of HCV by solid phase hybridization with fluorescent probe

Journal of Hepatology ◽

10.1016/s0168-8278(01)80394-9 ◽

2001 ◽

Vol 34 (0) ◽

pp. 110

Author(s):

E Ilina

Keyword(s):

Fluorescent Probe ◽

Solid Phase ◽

Direct Detection

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A vacuum chamber for low background diffraction experiments

Zeitschrift für Kristallographie - Crystalline Materials ◽

10.1524/zkri.1996.211.9.591 ◽

1996 ◽

Vol 211 (9) ◽

Cited By ~ 1

Author(s):

R. B. Neder ◽

M. Burghammer ◽

Th. Grasl ◽

H. Schulz

Keyword(s):

Single Crystal ◽

Vacuum Chamber ◽

Single Crystal Diffraction ◽

Low Background

AbstractWe developed a small vacuum chamber for very low background single crystal diffraction experiments. The chamber has been designed for a large Eulerian cradle. The

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Evaluation of 1-Naphthol as a Convenient Fluorescent Probe for Monitoring Ethanol-induced Interdigitation in Lipid Bilayer Membrane¶

Photochemistry and Photobiology ◽

10.1562/0031-8655(2001)073<0573:eonaac>2.0.co;2 ◽

2001 ◽

Vol 73 (6) ◽

pp. 573 ◽

Cited By ~ 12

Author(s):

N. Pappayee ◽

Ashok K. Mishra

Keyword(s):

Fluorescent Probe ◽

Lipid Bilayer ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane

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A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

10.26434/chemrxiv.7176989 ◽

2018 ◽

Author(s):

Suying Xu ◽

Adam Sedgwick ◽

Souad Elfecky ◽

Wenbo Chen ◽

Ashley Jones ◽

...

Keyword(s):

Fluorescent Probe ◽

Boronic Acid ◽

Binding Constants ◽

Strong Fluorescence ◽

Fluorescence Response ◽

Turn On ◽

Fluorescence Turn On ◽

Hydrogel System

A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection. In solution, the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M).

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Single-Molecule Imaging of Active Mitochondrial Nitroreductases using a Photo-Crosslinking Fluorescent Sensor

10.26434/chemrxiv.7992980.v1 ◽

2019 ◽

Author(s):

Zacharias Thiel ◽

Pablo Rivera-Fuentes

Keyword(s):

Subcellular Localization ◽

Fluorescent Probe ◽

Single Molecule ◽

Mammalian Cells ◽

Fluorescent Sensor ◽

Biological Functions ◽

Localization Microscopy ◽

Drug Activation ◽

Nitroreductase Activity ◽

Single Molecule Localization Microscopy

Many biomacromolecules are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biological functions. Nitroreductases are enzymes capable of reducing nitro groups to amines and play a role in detoxification and pro-drug activation. Although nitroreductase activity has been detected in mammalian cells, the subcellular localization of this activity remains incompletely characterized. Here, we report a fluorescent probe that enables super-resolved imaging of pools of nitroreductase activity within mitochondria. This probe is activated sequentially by nitroreductases and light to give a photo-crosslinked adduct of active enzymes. In combination with a general photoactivatable marker of mitochondria, we performed two-color, threedimensional, single-molecule localization microscopy. These experiments allowed us to image the sub-mitochondrial organization of microdomains of nitroreductase activity.

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