A highly sensitive and selective fluorescence off–on probe for the detection of intracellular endogenous tyrosinase activity

2017 ◽  
Vol 53 (16) ◽  
pp. 2443-2446 ◽  
Author(s):  
Xiaofeng Wu ◽  
Xiaohua Li ◽  
Hongyu Li ◽  
Wen Shi ◽  
Huimin Ma
Keyword(s):  
Fluorescent Probe ◽  
High Sensitivity ◽  
Tyrosinase Activity ◽  
Background Signal ◽  
Low Background ◽  
Highly Sensitive

A fluorescent probe with a low background signal and high sensitivity is developed and applied to image the relative levels of tyrosinase activity in different cells.

Highly sensitive, stable, and precise detection of dopamine with carbon dots/tyrosinase hybrid as fluorescent probe

RSC Advances ◽  
2014 ◽  
Vol 4 (87) ◽  
pp. 46437-46443 ◽  
Author(s):  
Hao Li ◽  
Juan Liu ◽  
Manman Yang ◽  
Weiqian Kong ◽  
Hui Huang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Low Cost ◽  
High Sensitivity ◽  
Highly Sensitive

The carbon dots/tyrosinase hybrid as a low-cost fluorescent probe for the detection of dopamine exhibits high sensitivity, stability, and precision.

A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5472-5478 ◽  
Author(s):  
Peng Zhang ◽  
Shasha Li ◽  
Caixia Fu ◽  
Qian Zhang ◽  
Yuzhe Xiao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Near Infrared ◽  
High Sensitivity ◽  
Tyrosinase Activity ◽  
Cyanine Dye ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe for TYR activity with high sensitivity was developed based on the H-aggregation of a cyanine dye.

scholarly journals High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

2020 ◽  
Vol 21 (21) ◽  
pp. 7896
Author(s):  
Jun Nakayama ◽  
Ryohei Saito ◽  
Yusuke Hayashi ◽  
Nobuo Kitada ◽  
Shota Tamaki ◽  
...  
Keyword(s):  
Single Cell ◽  
In Vivo Imaging ◽  
Cancer Metastasis ◽  
Near Infrared ◽  
High Sensitivity ◽  
Single Cell Level ◽  
Background Signal ◽  
Cell Level ◽  
Highly Sensitive

Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.

scholarly journals Electrochemiluminescence Imaging for Bioanalysis

2019 ◽  
Vol 12 (1) ◽  
pp. 275-295 ◽  
Author(s):  
Jingjing Zhang ◽  
Stéphane Arbault ◽  
Neso Sojic ◽  
Dechen Jiang
Keyword(s):  
High Throughput ◽  
Single Cells ◽  
Rapid Development ◽  
High Sensitivity ◽  
Optical Excitation ◽  
Detection Sensitivity ◽  
Background Signal ◽  
Optical Elements ◽  
Low Background ◽  
Analytical Technique

Electrochemiluminescence (ECL) is a widely used analytical technique with the advantages of high sensitivity and low background signal. The recent and rapid development of electrochemical materials, luminophores, and optical elements significantly increases the ECL signals and, thus, ECL imaging with enhanced spatial and temporal resolutions is realized. Currently, ECL imaging is successfully applied to high-throughput bioanalysis and to visualize the distribution of molecules at single cells. Compared with other optical bioassays, no optical excitation is involved in imaging, so the approach avoids a background signal from illumination and increases the detection sensitivity. This review highlights some of the most exciting developments in this field, including the mechanisms, electrode designs, and the applications of ECL imaging in bioanalysis and at single cells and particles.

scholarly journals Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2241 ◽  
Author(s):  
Ewelina Zaorska ◽  
Marek Konop ◽  
Ryszard Ostaszewski ◽  
Dominik Koszelewski ◽  
Marcin Ufnal
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
High Sensitivity ◽  
Electrochemical Analysis ◽  
Azido Group ◽  
Ample Evidence ◽  
Sodium Phosphate Buffer ◽  
Highly Sensitive ◽  
H2s Detection ◽  
Endogenous Enzymes

Ample evidence suggests that H2S is an important biological mediator, produced by endogenous enzymes and microbiota. So far, several techniques including colorimetric methods, electrochemical analysis and sulfide precipitation have been developed for H2S detection. These methods provide sensitive detection, however, they are destructive for tissues and require tedious sequences of preparation steps for the analyzed samples. Here, we report synthesis of a new fluorescent probe for H2S detection, 4-methyl-2-oxo-2H-chromen-7-yl 5-azidopentanoate (1). The design of 1 is based on combination of two strategies for H2S detection, i.e., reduction of an azido group to an amine in the presence of H2S and intramolecular lactamization. Finally, we measured salivary H2S concentration in healthy, 18–40-year-old volunteers immediately after obtaining specimens. The newly developed self-immolative coumarin-based fluorescence probe (C15H15N3O4) showed high sensitivity to H2S detection in both sodium phosphate buffer at physiological pH and in saliva. Salivary H2S concentration in healthy volunteers was within a range of 1.641–7.124 μM.

scholarly journals A label-free and sensitive photoluminescence sensing platform based on long persistent luminescence nanoparticles for the determination of antibiotics and 2,4,6-trinitrophenol

RSC Advances ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 5714-5720 ◽  
Author(s):  
Xi Zhang ◽  
Na-Yan Xu ◽  
Qiong Ruan ◽  
Dan-Qing Lu ◽  
Yun-Hui Yang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Persistent Luminescence ◽  
Label Free ◽  
Low Background ◽  
Background Interference ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Visualized Detection

A highly sensitive luminescence sensor based on PLNPs for visualized detection of antibiotic and organic explosives was developed, which could eliminate the background interference, allowing low background and therefore high sensitivity.

scholarly journals A highly sensitive fluorescent probe for ozone based on coumarin-benzothiazole derivative

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kangni Chen ◽  
Yanbo Li ◽  
Jinyan Shang ◽  
Chao Zhao ◽  
Heping Li
Keyword(s):  
Fluorescent Probe ◽  
High Sensitivity ◽  
Water Soluble ◽  
Benzothiazole Derivatives ◽  
Efficient Detection ◽  
Highly Sensitive ◽  
New Type ◽  
Human Trachea ◽  
Influence Of Ph ◽  
High Sensitivity Detection

AbstractOzone is widely used in daily life, but studies have shown that O3 can damage human trachea and lungs, leading to diseases such as asthma, emphysema, and bronchitis. Therefore, it is of great significance to develop a simple and efficient detection method for monitoring O3 in living cells. In this study, 3-(but-3-en-1-yl)-2-(7-(but-3-en-1-yloxy)-2-oxo-2H-chromen-3-yl)benzo[d]thiazol-3-ium (BCT) as a new type of water-soluble fluorescent probe was synthesized by substitution reaction of 4-bromo-1-butene and hydroxycoumarin-benzothiazole derivatives, which can specifically detect ozone in aqueous solution. The interaction of ozone on the probe can be completed within 20 min, the fluorescence intensity is significantly enhanced, and it has the advantages of high sensitivity (detection limit LOD = 43 nM). The influence of pH on the fluorescent performance of BCT shows that the probe with super stability under weak alkali and acidic environment, which provides the necessary conditions for its detection of ozone in physiological system detection. Therefore, BCT is expected to become an effective tool for detecting ozone in cellular organisms.

High-sensitivity detection of gold labels by high-angle dark-field STEM imaging

1990 ◽  
Vol 48 (3) ◽  
pp. 892-893 ◽  
Author(s):  
Max T. Otten
Keyword(s):  
High Sensitivity ◽  
Dark Field ◽  
Bright Field ◽  
Backscattered Electron Imaging ◽  
Backscattered Electrons ◽  
Average Atomic Number ◽  
Highly Sensitive ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
High Sensitivity Detection

Labelling of antibodies with small gold probes is a highly sensitive technique for detecting specific molecules in biological tissue. Larger gold probes are usually well visible in TEM or STEM Bright-Field images of unstained specimens. In stained specimens, however, the contrast of the stain is frequently the same as that of the gold labels, making it virtually impossible to identify the labels, especially when smaller gold labels are used to increase the sensitivity of the immunolabelling technique. TEM or STEM Dark-Field images fare no better (Figs. 1a and 2a), again because of the absence of a clear contrast difference between gold labels and stain.Potentially much more useful is backscattered-electron imaging, since this will show differences in average atomic number which are sufficiently large between the metallic gold and the stains normally used. However, for the thin specimens and at high accelerating voltages of the STEM, the yield of backscattered electrons is very small, resulting in a very weak signal. Consequently, the backscattered-electron signal is often too noisy for detecting small labels, even for large spot sizes.

A phenanthrene[9,10-d]imidazole-phenol-based fluorescent probe combined ESIPT and AIE for “turn-on” detection of Cu2+ with a green-emitting and improved stokes shift and its application

2021 ◽  
Author(s):  
Jiahui Du ◽  
Bing Zhao ◽  
Wei Kan ◽  
Haochun Yin ◽  
Tianshu Song ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
Fluorescent Sensors ◽  
Turn On ◽  
Highly Sensitive

Development of highly sensitive and selective fluorescent sensors toward Cu2+ has gained considerable attention in view of its application of environmental and biological fields. However, the strategy of sensing by...

scholarly journals Role of MRI evaluation in acute secondary inability to walk in children

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
K. H. Sedeek ◽  
K. Aboualfotouh ◽  
S. M. Hassanein ◽  
N. M. Osman ◽  
M. H. Shalaby
Keyword(s):  
Transverse Myelitis ◽  
High Sensitivity ◽  
Acute Disseminated Encephalomyelitis ◽  
Limb Weakness ◽  
Non Invasive ◽  
Highly Sensitive ◽  
Common Causes ◽  
Bilateral Lower Limb ◽  
Severity Of The Disease

Abstract Background Acute bilateral lower limb weakness is a common problem in children which necessitates a rapid method for diagnosis. MRI is a non-invasive imaging technique that produces high-quality images of the internal structure of the brain and spinal cord. Results MRI was very helpful in reaching rapid and prompt diagnosis in children with acute inability to walk. Acute disseminated encephalomyelitis (ADEM), Guillain–Barré syndrome (GBS), and acute transverse myelitis (ATM) were the most common causes in our study. MRI proved to be of high sensitivity in detecting the lesions and reaching the diagnosis in ADEM and GBS; however, there was no significant relation between the lesions’ size, enhancement pattern, and severity of the disease or prognosis, yet in ATM the site of the lesion and number of cord segment affection were significantly related to the severity of the disease and prognosis. Conclusion MRI is a quick tool to reach the diagnosis of children with acute secondary inability to walk, and to eliminate other differential diagnosis which is essential for proper treatment and rapid full recovery. It is highly sensitive in detecting the lesions, their site and size.

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