Förster Resonance Energy Transfer-Based Fluorescent Probe for the Selective Imaging of Hydroxylamine in Living Cells

2019 ◽  
Vol 91 (17) ◽  
pp. 11397-11402 ◽  
Author(s):  
Baoli Dong ◽  
Minggang Tian ◽  
Xiuqi Kong ◽  
Wenhui Song ◽  
Yaru Lu ◽  
...  
2008 ◽  
Vol 363 (1500) ◽  
pp. 2143-2151 ◽  
Author(s):  
Kazuhiro Aoki ◽  
Etsuko Kiyokawa ◽  
Takeshi Nakamura ◽  
Michiyuki Matsuda

Fluorescence probes based on the principle of Förster resonance energy transfer (FRET) have shed new light on our understanding of signal transduction cascades. Among them, unimolecular FRET probes containing fluorescence proteins are rapidly increasing in number because these genetically encoded probes can be easily loaded into living cells and allow simple acquisition of FRET images. We have developed probes for small GTPases, tyrosine kinases, serine–threonine kinases and phosphoinositides. Images obtained with these probes have revealed that membrane protrusions such as nascent lamellipodia or neurites provide an active signalling platform in the growth factor-stimulated cells.


2020 ◽  
Vol 8 (40) ◽  
pp. 14125-14137
Author(s):  
Ronald Merckx ◽  
Thomas Swift ◽  
Ryan Rees ◽  
Joachim F. R. Van Guyse ◽  
Ella Schoolaert ◽  
...  

A well-defined FRET system based on heterotelechelic poly(2-ethyl-2-oxazoline) containing pyrene and coumarin 343 was successfully used as fluorescent probe for temperature sensing and processed into fibres and films showing excellent FRET efficiency.


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