Long-Term Feeding of Elemental Sulfur Alters Microbial Community Structure and Eliminates Mercury Methylation Potential in Sulfate-Reducing Bacteria Abundant Activated Sludge

2018 ◽  
Vol 52 (8) ◽  
pp. 4746-4753 ◽  
Author(s):  
Jin-ting Wang ◽  
Liang Zhang ◽  
Yuan Kang ◽  
Guanghao Chen ◽  
Feng Jiang
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Activated Sludge ◽  
Microbial Community Structure ◽  
Elemental Sulfur ◽  
Sulfate Reducing Bacteria ◽  
Mercury Methylation ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM BIOFILM SULFATE REDUCTION AND CU PRECIPITATION BIOREACTOR (BATCH CULTURE).

2018 ◽  
Vol 2 (2) ◽  
pp. 1
Author(s):  
Tyas Nyonita Punjungsari
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Sulfate Reduction ◽  
Batch Culture ◽  
Microbial Community Structure ◽  
Sulfate Reducing Bacteria ◽  
Zeolite Surface ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The biofilm is a microbial community structure formed on the zeolite surface in a sulfate reduction bioreactor and Cu deposition using a SRB consortium . The biofilm soluble microbial solvent is expected to have the capability in sulfate reduction and Cu deposition. Characterization of isolates is required for the optimization of pure culture . The aim of this study is to isolate and characterize the biofilm sulfate reducing bacteria in the sulfate reduction bioreactor and the precipitation of Cu in Batch Culture by a consortium of Sulfate Reducing Bacteria. The method used in this study cultivation was done by using postgate B medium, isolation was done by diluting biofilm on NaCl salt, bacteria grown on NB and postgate B media, characterization done by morphology and biochemistry. There were 3 isolates of B1 (Metylobacterium ), B3 ( Desulfucoccus ), and B2 ( Desulfobacter ). B3 ( Desulfococcus) has the best ability to reduce sulfate and Cu precipitation.Keywords : Sulfur Reducing Bacteria (SRB), Biofilm, Sulfate, Cu. Received: 26 August, 2017; Accepter: 10 September, 2017 

scholarly journals Detection of sulfate-reducing bacteria as an indicator for successful mitigation of sulfide production

2021 ◽  
Author(s):  
Avishek Dutta ◽  
Fernando Valle ◽  
Thomas Goldman ◽  
Jeff Keating ◽  
Ellen Burke ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Critical Time ◽  
Sulfate Reducing Bacteria ◽  
Time Points ◽  
Sulfate Reducing ◽  
Nitrate Treatment ◽  
Reducing Bacteria ◽  
Planktonic Community

Sulfate-reducing bacteria (SRB) are one of the main sources of biogenic H 2 S generation in oil reservoirs. Excess H 2 S production in these systems leads to oil biosouring, which causes operational risks, health hazards and can increase the cost of refining crude oil. Nitrate salts are often added to the system to suppress sulfidogenesis. Because SRB populations can persist in biofilms even after nitrate treatment, identifying shifts in the sessile community is crucial for successful mitigation. However, sampling the sessile community is hampered by its inaccessibility. Here we use the results of a long-term (148 days) ex situ experiment to identify particular sessile community members from observations of the sample waste stream. Microbial community structure was determined for 731 samples across twenty bioreactors using 16S rRNA gene sequencing. By associating microbial community structure with specific steps in the mitigation process, we could distinguish between taxa associated with H 2 S production and mitigation. After initiation of nitrate treatment, certain SRB populations increased in the planktonic community during critical time points, indicating the dissociation of SRBs from the biofilm. Predicted relative abundances of the dissimilatory sulfate reduction pathway also increased during the critical time points. Here, by analyzing the planktonic community structure, we describe a general method that uses high-throughput amplicon sequencing, metabolic inferences, and cell abundance data to identify successful biofilm mitigation. We anticipate that our approach is also applicable to other systems where biofilms must be mitigated but cannot be easily sampled. Importance Microbial biofilms are commonly present in many industrial processes and can negatively impact performance and safety. Within the oil industry, subterranean biofilms cause biosouring with implications for oil quality, cost, occupational health, and the environment. Because these biofilms cannot be directly sampled, methods are needed to indirectly assess the success of mitigation measures. This study demonstrates how the planktonic microbial community can be used to assess the dissociation of SRB-containing biofilms. We found that an increase in the abundance of a specific SRB population in the effluent after nitrate treatment can be used as a potential indicator for the successful mitigation of biofilm-forming SRBs. Moreover, a method for determining critical time points for detecting potential indicators is suggested. This study expands our knowledge in improving mitigation strategies for biosouring and could have broader implications in other systems where biofilms lead to adverse consequences.

scholarly journals Methane-related changes in prokaryotes along geochemical profiles in sediments of Lake Kinneret (Israel)

2015 ◽  
Vol 12 (10) ◽  
pp. 2847-2860 ◽  
Author(s):  
I. Bar-Or ◽  
E. Ben-Dov ◽  
A. Kushmaro ◽  
W. Eckert ◽  
O. Sivan
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Methane Oxidation ◽  
Microbial Community Structure ◽  
Iron Reduction ◽  
Sulfate Reducing Bacteria ◽  
Lake Kinneret ◽  
Anaerobic Oxidation Of Methane ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Abstract. Microbial methane oxidation is the primary control on the emission of the greenhouse gas methane into the atmosphere. In terrestrial environments, aerobic methanotrophic bacteria are largely responsible for this process. In marine sediments, a coupling of anaerobic oxidation of methane (AOM) with sulfate reduction, often carried out by a consortium of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria, consumes almost all methane produced within those sediments. Motivated by recent evidence for AOM with iron(III) in Lake Kinneret sediments, the goal of the present study was to link the geochemical gradients in the lake porewater to the microbial community structure. Screening of archaeal 16S rRNA gene sequences revealed a shift from hydrogenotrophic to acetoclastic methanogens with depth. The observed changes in microbial community structure suggest possible direct and indirect mechanisms for the AOM coupled to iron reduction in deep sediments. The percentage of members of the Nitrospirales order increased with depth, suggesting their involvement in iron reduction together with Geobacter genus and "reverse methanogenesis". An indirect mechanism through sulfate and ANME seems less probable due to the absence of ANME sequences. This is despite the abundant sequences related to sulfate-reducing bacteria (Deltaproteobacteria) together with the occurrence of dsrA in the deep sediment that could indicate the production of sulfate (disproportionation) from S0 for sulfate-driven AOM. The presence of the functional gene pmoA in the deep anoxic sediment together with sequences related to Methylococcales suggests the existence of a second unexpected indirect pathway – aerobic methane oxidation pathway in an anaerobic environment.

scholarly journals CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM BIOFILM SULFATE REDUCTION AND CU PRECIPITATION BIOREACTOR (BATCH CULTURE).

2018 ◽  
Vol 2 (2) ◽  
pp. 1
Author(s):  
Tyas Nyonita Punjungsari
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Sulfate Reduction ◽  
Batch Culture ◽  
Microbial Community Structure ◽  
Sulfate Reducing Bacteria ◽  
Zeolite Surface ◽  
Sulfate Reducing ◽  
Reducing Bacteria

The biofilm is a microbial community structure formed on the zeolite surface in a sulfate reduction bioreactor and Cu deposition using a SRB consortium . The biofilm soluble microbial solvent is expected to have the capability in sulfate reduction and Cu deposition. Characterization of isolates is required for the optimization of pure culture . The aim of this study is to isolate and characterize the biofilm sulfate reducing bacteria in the sulfate reduction bioreactor and the precipitation of Cu in Batch Culture by a consortium of Sulfate Reducing Bacteria. The method used in this study cultivation was done by using postgate B medium, isolation was done by diluting biofilm on NaCl salt, bacteria grown on NB and postgate B media, characterization done by morphology and biochemistry. There were 3 isolates of B1 (Metylobacterium ), B3 ( Desulfucoccus ), and B2 ( Desulfobacter ). B3 ( Desulfococcus) has the best ability to reduce sulfate and Cu precipitation.Keywords : Sulfur Reducing Bacteria (SRB), Biofilm, Sulfate, Cu. Received: 26 August, 2017; Accepter: 10 September, 2017

scholarly journals Microbial community structure with trends in methylation gene diversity and abundance in mercury-contaminated rice paddy soils in Guizhou, China

2018 ◽  
Vol 20 (4) ◽  
pp. 673-685 ◽  
Author(s):  
Tatiana A. Vishnivetskaya ◽  
Haiyan Hu ◽  
Joy D. Van Nostrand ◽  
Ann M. Wymore ◽  
Xiaohang Xu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Gene Diversity ◽  
Rice Paddy ◽  
Paddy Soils ◽  
Rice Paddies ◽  
Sulfate Reducing ◽  
Rice Paddy Soils ◽  
Reducing Bacteria

Sulfate-reducing bacteria and methanogens are the primary Hg-methylators in Chinese rice paddies.

Separating and characterizing functional nitrogen degraders via magnetic-nanoparticle mediated isolation technology in high concentration of ammonia nitrogen wastewater treatment

2020 ◽  
Author(s):  
Meng Yin ◽  
Yujiao Sun ◽  
Danyang Zheng ◽  
Lei Wang ◽  
Xiaohui Zhao ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Magnetic Nanoparticles ◽  
Activated Sludge ◽  
Microbial Community Structure ◽  
Magnetic Nanoparticle ◽  
Ammonia Nitrogen ◽  
Stable Conditions

<p>Magnetic-nanoparticle mediated isolation (MMI) is a new method for isolating active functional microbes from complex microorganisms without substrate labeling. In this study, the composition and properties of the magnetic nanoparticles （MNPs）were characterized by a number of techniques. And then the MNPs were added to activated sludge rich in ammonia nitrogen-degrading bacteria after long-term stable treatment,  another set of experiments plus urea was set as the only carbon source in the system. Compared with the group without MNPs, degradation experiment results showed that the ammonia nitrogen degradation ability of a group of MNPs was slightly improved. The high-throughput sequencing results showed that the addition of MNPs did not change the microbial community structure of activated sludge under long-term stable conditions, and that the addition of urea as a nitrogen source significantly changed the microbial community structure. RDA analysis results also showed that Comamonadaceae_unclassified and Thiobacillus absolutely dominated in situ ammonia degradation, and the change in dominant genera showed the same trend as the degradation rate of ammonia nitrogen. It has also proved that the complex flora after adding magnetic nanoparticles is more adaptable to newly introduced pollutants, using MMI to study pollutant-degrading microorganisms under in-situ conditions has a broad application prospect.</p>

Stress-responses of activated sludge and anaerobic sulfate-reducing bacteria sludge under long-term ciprofloxacin exposure

2019 ◽  
Vol 164 ◽  
pp. 114964 ◽  
Author(s):  
Huiqun Zhang ◽  
Shiliu Song ◽  
Yanyan Jia ◽  
Di Wu ◽  
Hui Lu
Keyword(s):  
Activated Sludge ◽  
Stress Responses ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Microbial community change of sulfate reduction and sulfur oxidation bacteria in the activated sludge cultivated with acetate and peptone

2006 ◽  
Vol 54 (8) ◽  
pp. 111-119 ◽  
Author(s):  
N. Miyazato ◽  
R. Yamamoto-Ikemoto ◽  
S. Takamatsu
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Sulfur Oxidation ◽  
Sulfate Reducing Bacteria ◽  
Community Change ◽  
Oxidation Activity ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Reducing Bacteria

The growth of sulfate reducing bacteria (SRB) and filamentous sulfur bacteria was monitored on a laboratory scale in activated sludge reactors using acetate and peptone as the artificial wastewater. When the artificial wastewater contained acetate and peptone, filamentous bacteria increased in the sludge and the SVI values increased. There was a good correlation between sulfate reducing activity and sulfur oxidation activity in the produced sludge. The microbial community change of filamentous sulfur bacteria and sulfate reducing bacteria was analyzed using the fluorescent in situ hybridization (FISH) method. The tendency for the growth of filamentous sulfur bacteria Thiothrix eikelboomii following the growth of SRB was observed. The percentage of SRB385- hybridized cells and DNMA657-hybridized cells found in the total cell area increased from 2–3% to 7–10% when the filamentous bulking occurred.

Sign in / Sign up

Export Citation Format

Share Document