scholarly journals Linking Engineered Gene Circuit Kinetic Modeling to Cellulose Biosynthesis Prediction in Escherichia coli: Toward Bioprocessing of Microbial Cell Factories

2020 ◽  
Vol 59 (10) ◽  
pp. 4659-4669
Author(s):  
Gizem Buldum ◽  
Argyro Tsipa ◽  
Athanasios Mantalaris
Keyword(s):  
Escherichia Coli ◽  
Kinetic Modeling ◽  
Microbial Cell ◽  
Cellulose Biosynthesis ◽  
Gene Circuit ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Reverse engineering of fatty acid-tolerant Escherichia coli identifies design strategies for robust microbial cell factories

2020 ◽  
Vol 61 ◽  
pp. 120-130 ◽  
Author(s):  
Yingxi Chen ◽  
Erin E. Boggess ◽  
Efrain Rodriguez Ocasio ◽  
Aric Warner ◽  
Lucas Kerns ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Reverse Engineering ◽  
Microbial Cell ◽  
Design Strategies ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Acid Tolerant

scholarly journals ESCHERICHIA COLI REDOX MUTANTS AS MICROBIAL CELL FACTORIES FOR THE SYNTHESIS OF REDUCED BIOCHEMICALS

2012 ◽  
Vol 3 (4) ◽  
pp. e201210019 ◽  
Author(s):  
Jimena A. Ruiz ◽  
Alejandra de Almeida ◽  
Manuel S. Godoy ◽  
Mariela P. Mezzina ◽  
Gonzalo N. Bidart ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Insights into the structure of Escherichia coli outer membrane as the target for engineering microbial cell factories

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Jianli Wang ◽  
Wenjian Ma ◽  
Xiaoyuan Wang
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Microbial Cell ◽  
Type I ◽  
Fermentation Products ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Type I Fimbriae ◽  
Regulation Mechanisms ◽  
Membrane Engineering

AbstractEscherichia coli is generally used as model bacteria to define microbial cell factories for many products and to investigate regulation mechanisms. E. coli exhibits phospholipids, lipopolysaccharides, colanic acid, flagella and type I fimbriae on the outer membrane which is a self-protective barrier and closely related to cellular morphology, growth, phenotypes and stress adaptation. However, these outer membrane associated molecules could also lead to potential contamination and insecurity for fermentation products and consume lots of nutrients and energy sources. Therefore, understanding critical insights of these membrane associated molecules is necessary for building better microbial producers. Here the biosynthesis, function, influences, and current membrane engineering applications of these outer membrane associated molecules were reviewed from the perspective of synthetic biology, and the potential and effective engineering strategies on the outer membrane to improve fermentation features for microbial cell factories were suggested.

Developing fourth-generation biofuels secreting microbial cell factories for enhanced productivity and efficient product recovery; a review

Fuel ◽  
2021 ◽  
Vol 298 ◽  
pp. 120858
Author(s):  
Sana Malik ◽  
Ayesha Shahid ◽  
Chen-Guang Liu ◽  
Aqib Zafar Khan ◽  
Muhammad Zohaib Nawaz ◽  
...  
Keyword(s):  
Product Recovery ◽  
Microbial Cell ◽  
Fourth Generation ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Establishing new microbial cell factories for sustainable bioprocesses

2012 ◽  
Vol 29 ◽  
pp. S75-S76
Author(s):  
Mhairi Workman ◽  
Philippe Holt ◽  
Xiaoying Liu
Keyword(s):  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Johanna Hausjell ◽  
Heidi Halbwirth ◽  
Oliver Spadiut
Keyword(s):  
Cytochrome P450s ◽  
Microbial Cell ◽  
Cytochrome P450 Reductase ◽  
Redox Partner ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recombinant Production ◽  
Membrane Bound ◽  
Essential Reactions ◽  
Synthesis Of Hormones

Cytochrome P450s (P450s) comprise one of the largest known protein families. They occur in every kingdom of life and catalyze essential reactions, such as carbon source assimilation, synthesis of hormones and secondary metabolites, or degradation of xenobiotics. Due to their outstanding ability of specifically hydroxylating complex hydrocarbons, there is a great demand to use these enzymes for biocatalysis, including applications at an industrial scale. Thus, the recombinant production of these enzymes is intensively investigated. However, especially eukaryotic P450s are difficult to produce. Challenges are faced due to complex cofactor requirements and the availability of a redox-partner (cytochrome P450 reductase, CPR) can be a key element to get active P450s. Additionally, most eukaryotic P450s are membrane bound which complicates the recombinant production. This review describes current strategies for expression of P450s in the microbial cell factories Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris.

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