Comprehensive Comparison of Two Color Varieties of Perillae Folium Using Rapid Resolution Liquid Chromatography Coupled with Quadruple-Time-of-Flight Mass Spectrometry (RRLC-Q/TOF-MS)-Based Metabolic Profile and in Vivo/in Vitro Anti-Oxidative Activity

2020 ◽  
Vol 68 (49) ◽  
pp. 14684-14697
Author(s):  
Yun-Feng Zheng ◽  
Dan-Yang Li ◽  
Jie Sun ◽  
Jian-Ming Cheng ◽  
Chuan Chai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Metabolic Profile ◽  
Time Of Flight ◽  
Rapid Resolution ◽  
Flight Mass Spectrometry ◽  
Comprehensive Comparison ◽  
Tof Ms

scholarly journals New Metabolites of Udenafil Identified through Liquid Chromatography– Quadrupole Time-of-flight Mass Spectrometry

2020 ◽  
Vol 17 ◽  
Author(s):  
Jaesung Pyo
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Solid Phase ◽  
Liver Microsomes ◽  
Time Of Flight ◽  
Rat Urine ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Background: Udenafil, a recently discovered drug used for erectile dysfunction treatment, has been widely prescribed and its effect on human systems has been extensively studied. However, there is little research on the human metabolites of udenafil. Three metabolites have been identified in rats. Objective: Herein, highly sensitive and accurate liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS) was conducted to identify new udenafil metabolites. Methods: Human liver microsomes were incubated with udenafil for in vitro samples, and rat urine and faeces samples were collected from udenafil-administered rats for in vivo samples. Each sample was deproteinated with acetonitrile and extracted by solid phase extraction. The purified samples were separated and analyzed by LC-Q-TOF-MS, and some metabolite candidates were reanalyzed for further structural analysis using LC-Q-TOF-MS/MS. Results: Eleven and three metabolites were identified in the in vitro and in vivo samples, respectively, and were found to be hydrolyzed, oxidized, or demethylated forms of udenafil or its metabolites. The error of the metabolic analysis was −8.7 to 7.6 ppm, indicating the high accuracy of the method. Conclusion: These metabolic results could be useful for further investigation of udenafil and new phosphodiesterase-5 inhibitors.

scholarly journals In Vitro/In Vivo Metabolism of Ginsenoside Rg5 in Rat Using Ultra-Performance Liquid Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2113 ◽  
Author(s):  
Chao Hong ◽  
Ping Yang ◽  
Shuping Li ◽  
Yizhen Guo ◽  
Dan Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
In Vivo Metabolism ◽  
Rat Urine ◽  
Flight Mass Spectrometry ◽  
Ginsenoside Rg5

Background: Ginsenoside Rg5 has been proved to have a wide range of pharmacological activities. However, the in vitro and in vivo metabolism pathways of ginsenosides are still unclear, which impedes the understanding of their in vivo fate. In this paper, the possible metabolic process of Rg5 was studied and the metabolites are identified. Methods: Samples from rat liver microsomes (RLMs) in vitro and from rat urine, plasma and feces in vivo were collected for analysis after oral administration of Rg5. A rapid analysis technique using ultra-performance liquid chromatography (UPLC)/quadrupole-time-of-flight mass spectrometry (QTOF-MS) was applied for detecting metabolites of Rg5 both in vitro and in vivo. Results: A feasible metabolic pathway was proposed and described for ginsenoside Rg5. A total of 17 metabolic products were detected in biological samples, including the RLMs (four), rat urine (two), feces (13) and plasma (four). Fifteen of them have never been reported before. Oxidation, deglycosylation, deoxidation, glucuronidation, demethylation and dehydration were found to be the major metabolic reactions of Rg5. Conclusions: The present study utilized a reliable and quick analytical tool to explore the metabolism of Rg5 in rats and provided significant insights into the understanding of the metabolic pathways of Rg5 in vitro and in vivo. The results could be used to not only evaluate the efficacy and safety of Rg5, but also identify potential active drug candidates from the metabolites.

Profile of phenolic compounds of Brazilian virgin olive oils by rapid resolution liquid chromatography coupled to electrospray ionisation time-of-flight mass spectrometry (RRLC–ESI-TOF-MS)

2015 ◽  
Vol 170 ◽  
pp. 366-377 ◽  
Author(s):  
Cristiano Augusto Ballus ◽  
Rosa Quirantes-Piné ◽  
Abdelhakim Bakhouche ◽  
Luiz Fernando de Oliveira da Silva ◽  
Adelson Francisco de Oliveira ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Time Of Flight ◽  
Virgin Olive Oils ◽  
Rapid Resolution ◽  
Olive Oils ◽  
Electrospray Ionisation ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Metabonomics study on nephrotoxicity induced by intraperitoneal and intravenous cisplatin administration using rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS)

RSC Advances ◽  
2014 ◽  
Vol 4 (16) ◽  
pp. 8260-8270 ◽  
Author(s):  
Yubo Li ◽  
Xiuxiu Zhang ◽  
Huifang Zhou ◽  
Simiao Fan ◽  
Yuming Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Time Of Flight ◽  
Rapid Resolution ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Metabonomics was used to find characteristics of nephrotoxicity induced by IP or IV injection of cisplatin.

Sign in / Sign up

Export Citation Format

Share Document