Combinatorial Engineering of Transcriptional Activators in Penicillium oxalicum for Improved Production of Corn-Fiber-Degrading Enzymes

2021 ◽  
Vol 69 (8) ◽  
pp. 2539-2548
Author(s):  
Liwei Gao ◽  
Xin He ◽  
Yingjie Guo ◽  
Zehua Wu ◽  
Jian Zhao ◽  
...  
Keyword(s):  
Penicillium Oxalicum ◽  
Corn Fiber ◽  
Transcriptional Activators ◽  
Degrading Enzymes ◽  
Combinatorial Engineering

Penicillium oxalicum secretomic analysis identify plant cell wall degrading enzymes important for fruit juice extraction

2020 ◽  
Author(s):  
Lucélia Santi ◽  
Walter O. Beys-da-Silva ◽  
Markus Berger ◽  
John R. Yates ◽  
Adriano Brandelli ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Fruit Juice ◽  
Penicillium Oxalicum ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes ◽  
Juice Extraction

scholarly journals ARTP/EMS-combined multiple mutagenesis efficiently improved production of raw starch-degrading enzymes in Penicillium oxalicum and characterization of the enzyme-hyperproducing mutant

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Li-Sha Gu ◽  
Ming-Zhu Tan ◽  
Shi-Huan Li ◽  
Ting Zhang ◽  
Qi-Qiang Zhang ◽  
...  
Keyword(s):  
Regulatory Gene ◽  
Random Mutagenesis ◽  
Culture Conditions ◽  
Penicillium Oxalicum ◽  
Biofuel Production ◽  
Nucleotide Polymorphisms ◽  
Raw Starch ◽  
Temperature Plasma ◽  
Cassava Flour ◽  
Degrading Enzymes

Abstract Background Application of raw starch-degrading enzymes (RSDEs) in starch processing for biofuel production can effectively reduce energy consumption and processing costs. RSDEs are generally produced by filamentous fungi, such as Penicillium oxalicum, but with very low yields, which seriously hampers industrialization of raw starch processing. Breeding assisted by random mutagenesis is an efficient way to improve fungal enzyme production. Results A total of 3532 P. oxalicum colonies were generated after multiple rounds of mutagenesis, by atmospheric and room-temperature plasma (ARTP) and/or ethyl methanesulfonate (EMS). Of these, one mutant A2-13 had the highest RSDE activity of 162.7 U/mL, using raw cassava flour as substrate, a yield increase of 61.1%, compared with that of the starting strain, OXPoxGA15A. RSDE activity of A2-13 further increased to 191.0 U/mL, through optimization of culture conditions. Increased expression of major amylase genes, including the raw starch-degrading glucoamylase gene, PoxGA15A, and its regulatory gene, PoxAmyR, as well as several single-nucleotide polymorphisms in the A2-13 genome, were detected by real-time reverse transcription quantitative PCR and genomic re-sequencing, respectively. In addition, crude RSDEs produced by A2-13, combined with commercial α-amylase, could efficiently digest raw corn flour and cassava flour at 40 °C. Conclusions Overall, ARTP/EMS-combined mutagenesis effectively improved fungal RSDE yield. An RSDE-hyperproducing mutant, A2-13, was obtained, and its RSDEs could efficiently hydrolyze raw starch, in combination with commercial α-amylase at low temperature, which provides a useful RSDE resource for future starch processing.

Enhanced expression of the transcriptional activators YAP and TAZ in non-secreting pituitary tumours

2018 ◽  
Author(s):  
Paraskevi Xekouki ◽  
Emily Lodge ◽  
Ran Li ◽  
Jorg Flitsch ◽  
Stefan Bornstein ◽  
...  
Keyword(s):  
Transcriptional Activators ◽  
Pituitary Tumours ◽  
Enhanced Expression

Effect of Olive Oil Mill Waste Waters on the Edible and Medicinal Mushroom Lentinus edodes (Berk.) Sing. Growth and Lignin Degrading Enzymes

2002 ◽  
Vol 4 (2) ◽  
pp. 10 ◽  
Author(s):  
Slaven Zjalic ◽  
Anna Adele Fabbri ◽  
Alessandra Ricelli ◽  
Massimo Reverberi ◽  
Emanuela Galli ◽  
...  
Keyword(s):  
Olive Oil ◽  
Medicinal Mushroom ◽  
Lentinus Edodes ◽  
Waste Waters ◽  
Degrading Enzymes ◽  
Mill Waste
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