Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

2021 ◽  
Author(s):  
Tong He ◽  
Jing Liu ◽  
Jian Ping Wang
Keyword(s):  
Fluorescence Polarization ◽  
Recognition Mechanism ◽  
Dihydropteroate Synthase ◽  
Fluorescence Polarization Assay

Development of a fluorescence polarization assay to screen for inhibitors of the FtsZ/ZipA interaction

2003 ◽  
Vol 323 (2) ◽  
pp. 224-233 ◽  
Author(s):  
Cynthia Hess Kenny ◽  
Weidong Ding ◽  
Kerry Kelleher ◽  
Susan Benard ◽  
Elizabeth Glasfeld Dushin ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Fluorescence Polarization Assay

A Fluorescence Polarization Assay for Cyclic Nucleotide Phosphodiesterases

2002 ◽  
Vol 7 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Wei Huang ◽  
Yan Zhang ◽  
J. Richard Sportsman
Keyword(s):  
Cyclic Nucleotide ◽  
Fluorescence Polarization ◽  
Drug Targets ◽  
Second Messengers ◽  
Reaction Products ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Extracellular Signals ◽  
Fluorescence Polarization Assay ◽  
Hydrolysis Of ◽  
Nucleotide Phosphodiesterases

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3′-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing interest in the study of PDEs as drug targets for novel therapeutics. We describe the development of a homogeneous fluorescence polarization assay for PDEs based on the strong binding of PDE reaction products (i.e., AMP or GMP) onto modified nanoparticles through interactions with immobilized trivalent metal cations. This assay technology (IMAP) is applicable to both cAMP- and cGMP-specific PDEs. Results of the assay in 384- and 1536-well microplates are presented.

Fluorescence Polarization Assay: Application to the Diagnosis of Bovine Brucellosis in Argentina

1999 ◽  
Vol 20 (3) ◽  
pp. 115-126 ◽  
Author(s):  
L. Samartino ◽  
R. Gregoret ◽  
D. Gall ◽  
K. Nielsen
Keyword(s):  
Fluorescence Polarization ◽  
Bovine Brucellosis ◽  
Fluorescence Polarization Assay

Assessment of fluorescence polarization assay: a candid diagnostic tool inBrucella abortusstrain 19 vaccinated areas

2018 ◽  
Vol 62 (11) ◽  
pp. 694-701 ◽  
Author(s):  
Triveni Kalleshamurthy ◽  
Ranjitha Shekar ◽  
Harish Heballi Niranjanamurthy ◽  
Krithiga Natesan ◽  
Bibek Ranjan Shome ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Assay

A Fluorescence Polarization Assay To Detect Steroid Hormone Traces in Milk

2015 ◽  
Vol 63 (41) ◽  
pp. 9159-9164 ◽  
Author(s):  
Antonio Varriale ◽  
Anna Pennacchio ◽  
Gabriella Pinto ◽  
Giorgia Oliviero ◽  
Stefano D’Errico ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Steroid Hormone ◽  
Fluorescence Polarization Assay

scholarly journals A high‐throughput fluorescence polarization assay for the determination of Kd for soluble epoxide hydrolase inhibitors

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Richard H. Ingraham ◽  
Rachel R. Kroe ◽  
Maurice M. Morelock ◽  
John R. Proudfoot ◽  
Anne B. Eldrup ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Polarization ◽  
Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase Inhibitors ◽  
Fluorescence Polarization Assay

scholarly journals Application of the Fluorescence Polarization Assay for Detection of Caprine Antibodies to Brucella melitensis in Areas of High Prevalence and Widespread Vaccination

2007 ◽  
Vol 14 (3) ◽  
pp. 299-303 ◽  
Author(s):  
C. Ramírez-Pfeiffer ◽  
K. Nielsen ◽  
P. Smith ◽  
F. Marín-Ricalde ◽  
C. Rodríguez-Padilla ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Screening Test ◽  
Brucella Melitensis ◽  
Low Cost ◽  
Animal Health ◽  
Screening Tests ◽  
Confirmatory Test ◽  
High Prevalence ◽  
Fluorescence Polarization Assay ◽  
World Organization

ABSTRACT The screening Rose Bengal test (RBT), the buffered plate agglutination test (BPAT), and the confirmatory complement fixation test (CFT) are currently approved by the World Organization for Animal Health (OIE) for diagnosis of goat brucellosis. However, RBT (at 3% or 8% cell concentration) is known to be affected by vaccinal antibodies. In the present study, Mexican and Canadian OIE tests were compared with the fluorescence polarization assay (FPA), alone or in combination, using indirect and competitive enzyme-linked immunosorbent assays as classification variables for goat sera obtained from an area of high prevalence and widespread vaccination. The relative sensitivities and specificities were, respectively, 99.7% and 32.5% for RBT3, 92.8% and 68.8% for RBT8, 98.4% and 84.8% for Canadian CFT, 83.7% and 65.5% for Mexican CFT, and 78.1% and 89.3% for FPA. The use of FPA as the confirmatory test in combination with other tests significantly increased the final specificities of the screening tests alone; BPAT, RBT3, and RBT8 plus FPA resulted in final specificities of 90%, 91.2%, and 91.3%, respectively, whereas for the combinations RBT3 plus Mexican CFT, RBT8 plus Mexican CFT, and BPAT plus Canadian CFT, specificities were 65.5%, 63.2%, and 91.7%, respectively. We suggest that FPA may be routinely applied as an adaptable screening test for diagnosis of goat brucellosis and as a confirmatory test for screening test series. Some advantages of FPA are that its cutoff can be adjusted to improve its sensitivity or specificity, it is a low-cost and easy-to-perform test of choice when specificity is relevant or when an alternative confirmatory test is not available, and it is not affected by vaccination, thus reducing the number of misdiagnosed and killed goats.

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