Quantitative Analysis of Protein Unfolded State Energetics: Experimental and Computational Studies Demonstrate That Non-Native Side-Chain Interactions Stabilize Local Native Backbone Structure

2021 ◽  
Vol 125 (13) ◽  
pp. 3269-3277
Author(s):  
Junjie Zou ◽  
Shifeng Xiao ◽  
Carlos Simmerling ◽  
Daniel P. Raleigh
Keyword(s):  
Quantitative Analysis ◽  
Side Chain ◽  
Computational Studies ◽  
Unfolded State ◽  
Backbone Structure

scholarly journals Glycan remodeled erythrocytes facilitate antigenic characterization of recent A/H3N2 influenza viruses

2020 ◽  
Author(s):  
Frederik Broszeit ◽  
Rosanne J. van Beek ◽  
Luca Unione ◽  
Theo M. Bestebroer ◽  
Digantkumar Chapla ◽  
...  
Keyword(s):  
Influenza Viruses ◽  
Side Chain ◽  
Computational Studies ◽  
Receptor Binding Site ◽  
Adaptive Mutations ◽  
Antibody Recognition ◽  
Antigenic Characterization ◽  
H3n2 Influenza ◽  
Human Receptor

AbstractDuring circulation in humans and natural selection to escape antibody recognition for decades, A/H3N2 influenza viruses emerged with altered receptor specificities. These viruses lost the ability to agglutinate erythrocytes critical for antigenic characterization and give low yields and acquire adaptive mutations when cultured in eggs and cells, contributing to recent vaccine challenges. We examined receptor specificities of A/H3N2 viruses, revealing that recent viruses compensated for decreased binding of the prototypic human receptor by recognizing 2,6-sialosides on extended LacNAc moieties. Erythrocyte glycomics showed an absence of extended glycans, providing a rationale for lack of agglutination by recent A/H3N2 viruses. A glycan remodeling approach installed functional receptors on erythrocytes, allowing antigenic characterization of recent A/H3N2 viruses and confirming the cocirculation of several antigenically different viruses in humans. Computational studies of HAs in complex with a sialoside having an extended LacNAc moiety revealed that mutations distal to the RBD reoriented the Y159 side chain, resulting in an extended receptor binding site.

Using Side-Chain Aromatic Proton Chemical Shifts for a Quantitative Analysis of Protein Structures

2011 ◽  
Vol 50 (41) ◽  
pp. 9620-9623 ◽  
Author(s):  
Aleksandr B. Sahakyan ◽  
Wim F. Vranken ◽  
Andrea Cavalli ◽  
Michele Vendruscolo
Keyword(s):  
Quantitative Analysis ◽  
Chemical Shifts ◽  
Protein Structures ◽  
Aromatic Proton ◽  
Side Chain ◽  
Proton Chemical Shifts

scholarly journals Quantitative Analysis of Cu(I) Concentration in Click Chemistry — Biotinylation at Side Chain of Propargylglycine Using Click Chemistry under Heating Conditions —

Heterocycles ◽  
2012 ◽  
Vol 86 (1) ◽  
pp. 735 ◽  
Author(s):  
Makoto Hashimoto ◽  
Yui Ogasawara ◽  
Yuta Murai ◽  
Yasuko Sakihama ◽  
Yasuyuki Hashidoko
Keyword(s):  
Quantitative Analysis ◽  
Click Chemistry ◽  
Side Chain

Alternation of Side-Chain Mesogen Orientation Caused by the Backbone Structure in Liquid-Crystalline Polymer Thin Films

Langmuir ◽  
2015 ◽  
Vol 31 (42) ◽  
pp. 11379-11383 ◽  
Author(s):  
Daisuke Tanaka ◽  
Yuki Nagashima ◽  
Mitsuo Hara ◽  
Shusaku Nagano ◽  
Takahiro Seki
Keyword(s):  
Thin Films ◽  
Liquid Crystalline Polymer ◽  
Liquid Crystalline ◽  
Polymer Thin Films ◽  
Crystalline Polymer ◽  
Side Chain ◽  
Backbone Structure

scholarly journals Impact of backbone composition on homopolymer dynamics and brush block copolymer self-assembly

2020 ◽  
Vol 11 (45) ◽  
pp. 7147-7158
Author(s):  
Bret M. Boyle ◽  
Joseph L. Collins ◽  
Tara E. Mensch ◽  
Matthew D. Ryan ◽  
Brian S. Newell ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Block Copolymers ◽  
Photonic Crystals ◽  
Self Assembly ◽  
Side Chain ◽  
Backbone Structure

Four series of brush block copolymers with near identical side chain compositions but varying backbone structures were synthesized to investigate the effect of backbone structure on the thermal self-assembly to photonic crystals.

Quantitative analysis of mixtures of toluene and its side-chain-chlorinated derivatives by gas chromatography

1975 ◽  
Vol 114 (1) ◽  
pp. 247-249 ◽  
Author(s):  
R. Ramakrishnan ◽  
N. Subramanian
Keyword(s):  
Gas Chromatography ◽  
Quantitative Analysis ◽  
Side Chain

NMR Characterization of Side Chain Flexibility and Backbone Structure in the Type I Antifreeze Protein at Near Freezing Temperatures†

Biochemistry ◽  
1996 ◽  
Vol 35 (51) ◽  
pp. 16698-16704 ◽  
Author(s):  
Wolfram Gronwald ◽  
Heman Chao ◽  
D. Venkat Reddy ◽  
Peter L. Davies ◽  
Brian D. Sykes ◽  
...  
Keyword(s):  
Antifreeze Protein ◽  
Side Chain ◽  
Type I ◽  
Chain Flexibility ◽  
Nmr Characterization ◽  
Backbone Structure ◽  
Freezing Temperatures
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