This study investigated transient effects on the air quality of parked cars and moving cars with and without operating air conditioning. Carbon dioxide, carbon monoxide, volatile organic compounds, and formaldehyde concentrations were measured for comparative analysis. The results showed that simply changing the air conditioning system from internal circulation to external circulation to introduce air from outside reduces carbon dioxide concentrations by more than 50%, volatile organic compound concentrations by more than 77%, and the heat index from 0.1℃/min to less than 0.05 ℃/min. In order to conserve energy and improve car indoor air quality, this study can serve as a reference on healthy car environments.