Bioelectrocatalytic Activity of W-Formate Dehydrogenase Covalently Immobilized on Functionalized Gold and Graphite Electrodes

Chicken manure wastewaters are increasingly being considered a valuable resource of organic compounds. Screened chicken manure was evaluated as a representative solid organic waste. In this study, electricity generation from livestock wastewater (chicken manure) was investigated in a continuous mediator-less horizontal flow microbial fuel cell with graphite electrodes and a selective type of membrane separating the anodic and cathodic compartments of MFC from each other. The performance of MFC was evaluated to livestock wastewater using aged anaerobic sludge. Results revealed that COD and BOD removal efficiencies were up to 88% and 82%, respectively. At an external resistance value of 150 Ω, a maximum power and current densities of 278 m.W/m2 and 683 mA/m2, respectively were obtained, hence MFC utilizing livestock wastewater would be a sustainable and reliable source of bio-energy generation .

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Faculty Opinions recommendation of Molecular basis of proton motive force generation: structure of formate dehydrogenase-N.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004770.61154 ◽

2002 ◽

Author(s):

Edward Stiefel

Keyword(s):

Molecular Basis ◽

Formate Dehydrogenase ◽

Proton Motive Force ◽

Motive Force ◽

Force Generation ◽

Generation Structure

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Faculty Opinions recommendation of Molecular basis of proton motive force generation: structure of formate dehydrogenase-N.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004770.55254 ◽

2002 ◽

Author(s):

Jorge Escalante-Semerena

Keyword(s):

Molecular Basis ◽

Formate Dehydrogenase ◽

Proton Motive Force ◽

Motive Force ◽

Force Generation ◽

Generation Structure

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Faculty Opinions recommendation of Selenite assimilation into formate dehydrogenase H depends on thioredoxin reductase in Escherichia coli.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1104860.560947 ◽

2008 ◽

Author(s):

Russ Hille

Keyword(s):

Escherichia Coli ◽

Formate Dehydrogenase ◽

Thioredoxin Reductase

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Expression of the NADP+-Dependent Formate Dehydrogenase Gene from Pseudomonas Increases the Lysine Production in Corynebacterium glutamicum

Biotekhnologiya ◽

10.21519/0234-2758-2019-35-6-21-29 ◽

2019 ◽

Vol 35 (6) ◽

pp. 21-29

Author(s):

T.E. Leonova ◽

T.E. Shustikova ◽

T.V. Gerasimova ◽

Т.А. Ivankova ◽

K.V. Sidorenko Sidorenko ◽

...

Keyword(s):

Corynebacterium Glutamicum ◽

Formate Dehydrogenase ◽

Ministry Of Education ◽

Lysine Production ◽

Simultaneous Formation ◽

Resource Center ◽

Formate Oxidation ◽

Gene Coding ◽

Dehydrogenase Gene ◽

Lysine Synthesis

Thepsefdh_D221Q gene coding for a mutant formate dehydrogenase (PseFDG_D221Q) from Pseudomonas, which catalyzes the formate oxidation with the simultaneous formation of NADPH, has been expressed in the cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into С. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with simultaneous inactivation of host formate dehydrogenase genes. It was shown that the С. glutamicum strains with NADP+ -dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate, if their own formate dehydrogenase is inactivated. L-lysine, formate dehydrogenase, NADPH, Corynebacterium glutamicum The work was carried out using the equipment of the Multipurpose Scientific This work was carried out on the equipment of the Multipurpose Scientific Installation of «All-Russian Collection of Industrial Microorganisms», National Bio-Resource Center, NRC «Kurchatov Institute»- GosNIIgenetika. This work was financially supported by the Ministry of Education and Science of Russia (Unique Project Identifier - RFMEFI61017X0011).

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