Near-Infrared Light Activation of Proteins Inside Living Cells Enabled by Carbon Nanotube-Mediated Intracellular Delivery

2016 ◽  
Vol 8 (7) ◽  
pp. 4500-4507 ◽  
Author(s):  
He Li ◽  
Xinqi Fan ◽  
Xing Chen
Keyword(s):  
Carbon Nanotube ◽  
Near Infrared ◽  
Intracellular Delivery ◽  
Living Cells ◽  
Infrared Light ◽  
Light Activation ◽  
Near Infrared Light

scholarly journals The bactericidal effect of carbon nanotube/agar composites irradiated with near-infrared light on Streptococcus mutans

2010 ◽  
Vol 173 (1-3) ◽  
pp. 187-190 ◽  
Author(s):  
Tsukasa Akasaka ◽  
Makoto Matsuoka ◽  
Takeshi Hashimoto ◽  
Shigeaki Abe ◽  
Motohiro Uo ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Streptococcus Mutans ◽  
Near Infrared ◽  
Bactericidal Effect ◽  
Infrared Light ◽  
Near Infrared Light

scholarly journals A Visible and Near-Infrared Light Activatable Diazo-Coumarin Probe for Fluorogenic Protein Labeling in Living Cells

2020 ◽  
Author(s):  
Sheng-Yao Dai ◽  
Dan Yang
Keyword(s):  
Near Infrared ◽  
Uv Light ◽  
Cell Types ◽  
Living Cells ◽  
Infrared Light ◽  
Fluorescence Labeling ◽  
Insertion Reaction ◽  
Selective Labeling ◽  
Near Infrared Light ◽  
Endogenous Protein

Chemical modification of proteins in living cells permits valuable glimpses into the molecular interactions that underpin dynamic cellular events. While genetic engineering methods are often preferred, selective labeling of endogenous proteins in a complex intracellular milieu with chemical approaches represents a significant challenge. In this study, we report novel diazo-coumarin compounds that can be photo-activated by visible (430‒490 nm) and near-infrared light (800 nm) irradiation to photo-uncage reactive carbene intermediates, which could subsequently undergo insertion reaction with concomitant fluorescence “turned-on”. With these new molecules in hand, we have developed a new approach for rapid, selective and fluorogenic labeling of endogenous protein in living cells. By using CA-II and eDHFR as model proteins, we demonstrated that subcellular localization of proteins can be precisely visualized by live-cell imaging and protein levels can be reliably quantified in multiple cell types using flow cytometry. Dynamic protein regulations such as hypoxia induced CA-IX accumulation can also be detected. In addition, by two-photon excitation with an 800 nm laser, cell-selective labeling can also be achieved with spatially controlled irradiation. Our method circumvents the cytotoxicity of UV light and obviates the need for introducing external reporters with “click chemistries”. We believe that this approach of fluorescence labeling of endogenous protein by bioorthogonal photo-irradiation opens up exciting opportunities for discoveries and mechanistic interrogation in chemical biology.

Carbon nanotube-assisted optical activation of TGF-β signalling by near-infrared light

2015 ◽  
Vol 10 (5) ◽  
pp. 465-471 ◽  
Author(s):  
Liang Lin ◽  
Ling Liu ◽  
Bing Zhao ◽  
Ran Xie ◽  
Wei Lin ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Near Infrared ◽  
Infrared Light ◽  
Near Infrared Light ◽  
Optical Activation

Near-infrared light activation of quenched liposomal Ce6 for synergistic cancer phototherapy with effective skin protection

Biomaterials ◽  
2017 ◽  
Vol 127 ◽  
pp. 13-24 ◽  
Author(s):  
Liangzhu Feng ◽  
Danlei Tao ◽  
Ziliang Dong ◽  
Qian Chen ◽  
Yu Chao ◽  
...  
Keyword(s):  
Near Infrared ◽  
Infrared Light ◽  
Light Activation ◽  
Skin Protection ◽  
Near Infrared Light

Spatiotemporally Controllable MicroRNA Imaging in Living Cells via a Near-Infrared Light-Activated Nanoprobe

2020 ◽  
Vol 12 (32) ◽  
pp. 35958-35966
Author(s):  
Xianxian Zhao ◽  
Liangliang Zhang ◽  
Weiying Gao ◽  
Xingle Yu ◽  
Wei Gu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Living Cells ◽  
Infrared Light ◽  
Near Infrared Light

An intelligent near-infrared light activatable nanosystem for accurate regulation of zinc signaling in living cells

Nano Research ◽  
2017 ◽  
Vol 10 (9) ◽  
pp. 3068-3076 ◽  
Author(s):  
Wei Li ◽  
Zhen Liu ◽  
Zhaowei Chen ◽  
Lihua Kang ◽  
Yijia Guan ◽  
...  
Keyword(s):  
Near Infrared ◽  
Living Cells ◽  
Infrared Light ◽  
Near Infrared Light ◽  
Zinc Signaling
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