Plasmid DNA Delivery Using Cell-Penetrating Peptide Foldamers Composed of Arg–Arg–Aib Repeating Sequences

2019 ◽  
Vol 5 (11) ◽  
pp. 5660-5668 ◽  
Author(s):  
Makoto Oba ◽  
Yurika Ito ◽  
Tomohiro Umeno ◽  
Takuma Kato ◽  
Masakazu Tanaka
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

Plasmid DNA delivery by arginine-rich cell-penetrating peptides containing unnatural amino acids

2016 ◽  
Vol 24 (12) ◽  
pp. 2681-2687 ◽  
Author(s):  
Takuma Kato ◽  
Hiroko Yamashita ◽  
Takashi Misawa ◽  
Koyo Nishida ◽  
Masaaki Kurihara ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasmid Dna ◽  
Unnatural Amino Acids ◽  
Dna Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating

scholarly journals A simple and efficient transfection protocol for Cryptosporidium parvum using Polyethylenimine (PEI) and Octaarginine

Parasitology ◽  
2020 ◽  
Vol 147 (10) ◽  
pp. 1065-1070
Author(s):  
Tran Nguyen-Ho-Bao ◽  
Maxi Berberich ◽  
Wanpeng Zheng ◽  
Dieter Seebach ◽  
Arwid Daugschies ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cryptosporidium Parvum ◽  
Plasmid Dna ◽  
Cationic Polymer ◽  
Cell Penetrating Peptide ◽  
Laboratory Equipment ◽  
Short Cell ◽  
Transfection Protocol ◽  
Simple Protocol ◽  
Cell Penetrating

AbstractThe transfection of Cryptosporidium represents a major challenge, and current protocols are based on electroporation of freshly excysted sporozoites using a rather large amount of plasmid DNA which typically has a very poor yield. In this study, we report a fast and simple protocol for transfection of Cryptosporidium parvum that takes advantage of the DNA condensing power of the poly cationic polymer polyethylenimine (PEI) and the gene delivery property of the short cell-penetrating peptide octaarginine. Our novel protocol requires a very low amount of plasmid DNA and does not necessitate special laboratory equipment to be performed. Transfection appears to be more efficient in oocysts just triggered for excystation than the excysted sporozoites. Altogether, the application of octaarginine with PEI allows efficient transfection. To the best of our knowledge, this is the first report on an electroporation-free protocol for transfection of sporozoites of a Cryptosporidium species.

scholarly journals S413-PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

2008 ◽  
Vol 10 (11) ◽  
pp. 1210-1222 ◽  
Author(s):  
Sara Trabulo ◽  
Miguel Mano ◽  
Henrique Faneca ◽  
Ana Luísa Cardoso ◽  
Sónia Duarte ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Intracellular Delivery ◽  
Cationic Liposomes ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Pv Cell

scholarly journals Cell-Penetrating Peptide-Mediated Transformation of Large Plasmid DNA into Escherichia coli

2019 ◽  
Vol 8 (5) ◽  
pp. 1215-1218 ◽  
Author(s):  
Md Monirul Islam ◽  
Masaki Odahara ◽  
Takeshi Yoshizumi ◽  
Kazusato Oikawa ◽  
Mitsuhiro Kimura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasmid Dna ◽  
Cell Penetrating Peptide ◽  
Large Plasmid ◽  
Cell Penetrating

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

scholarly journals Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity

2020 ◽  
Vol 1 (2) ◽  
pp. 100014 ◽  
Author(s):  
Anja Henning-Knechtel ◽  
Sunil Kumar ◽  
Cecilia Wallin ◽  
Sylwia Król ◽  
Sebastian K.T.S. Wärmländer ◽  
...  
Keyword(s):  
Amyloid Beta ◽  
Peptide Inhibitors ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating
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