Quorum Sensing Inhibition Attenuates the Virulence of the Plant Pathogen Ralstonia solanacearum Species Complex

2020 ◽  
Vol 15 (11) ◽  
pp. 3050-3059
Author(s):  
Ayaka Yoshihara ◽  
Mika Shimatani ◽  
Megumi Sakata ◽  
Chika Takemura ◽  
Wakana Senuma ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Plant Pathogen ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Quorum Sensing Inhibition

scholarly journals Modulation of Quorum Sensing as an Adaptation to Nodule Cell Infection during Experimental Evolution of Legume Symbionts

mBio ◽  
2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Mingxing Tang ◽  
Olivier Bouchez ◽  
Stéphane Cruveiller ◽  
Catherine Masson-Boivin ◽  
Delphine Capela
Keyword(s):  
Quorum Sensing ◽  
Plant Pathogen ◽  
Ralstonia Solanacearum ◽  
Experimental Evolution ◽  
Root Nodules ◽  
Single Mutation ◽  
Content Type ◽  
Intracellular Infection ◽  
Infection Threads ◽  
Ancestral Strain

ABSTRACT Over millions of years, changes have occurred in regulatory circuitries in response to genome reorganization and/or persistent changes in environmental conditions. How bacteria optimize regulatory circuitries is crucial to understand bacterial adaptation. Here, we analyzed the experimental evolution of the plant pathogen Ralstonia solanacearum into legume symbionts after the transfer of a natural plasmid encoding the essential mutualistic genes. We showed that the Phc quorum sensing system required for the virulence of the ancestral bacterium was reconfigured to improve intracellular infection of root nodules induced by evolved Ralstonia. A single mutation in either the PhcB autoinducer synthase or the PhcQ regulator of the sensory cascade tuned the kinetics of activation of the central regulator PhcA in response to cell density so that the minimal stimulatory concentration of autoinducers needed for a given response was increased. Yet, a change in the expression of a PhcA target gene was observed in infection threads progressing in root hairs, suggesting early programming for the late accommodation of bacteria in nodule cells. Moreover, this delayed switch to the quorum sensing mode decreased the pathogenicity of the ancestral strain, illustrating the functional plasticity of regulatory systems and showing how a small modulation in signal response can produce drastic changes in bacterial lifestyle. IMPORTANCE Rhizobia are soil bacteria from unrelated genera able to form a mutualistic relationship with legumes. Bacteria induce the formation of root nodules, invade nodule cells, and fix nitrogen to the benefit of the plant. Rhizobial lineages emerged from the horizontal transfer of essential symbiotic genes followed by genome remodeling to activate and/or optimize the acquired symbiotic potential. This evolutionary scenario was replayed in a laboratory evolution experiment in which the plant pathogen Ralstonia solanacearum successively evolved the capacities to nodulate Mimosa pudica and poorly invade, then massively invade, nodule cells. In some lines, the improvement of intracellular infection was achieved by mutations modulating a quorum sensing regulatory system of the ancestral strain. This modulation that affects the activity of a central regulator during the earliest stages of symbiosis has a huge impact on late stages of symbiosis. This work showed that regulatory rewiring is the main driver of this pathogeny-symbiosis transition.

Signal Production and Response Specificity in the phc Quorum Sensing Systems of Ralstonia solanacearum Species Complex

2019 ◽  
Author(s):  
Yumeto Ujita ◽  
Megumi Sakata ◽  
Ayaka Yoshihara ◽  
Yasufumi Hikichi ◽  
Kenji Kai
Keyword(s):  
Quorum Sensing ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Sensing Systems ◽  
Signal Production ◽  
Response Specificity

Exploiting Quorum Sensing Inhibition for the Control of Pseudomonas aeruginosa and Acinetobacter baumannii Biofilms

2017 ◽  
Vol 17 (17) ◽  
pp. 1915-1927 ◽  
Author(s):  
Israel Castillo-Juarez ◽  
Luis Esau Lopez-Jacome ◽  
Gloria Soberon-Chavez ◽  
Maria Tomas ◽  
Jintae Lee ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Acinetobacter Baumannii ◽  
Quorum Sensing Inhibition

Quorum Sensing Inhibition by Phenothiazines and Related Compounds

2011 ◽  
Vol 8 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Zoltan Gabor Varga ◽  
Mira Agnes Szabo ◽  
Zsuzsanna Schelz ◽  
Erno Szegedi ◽  
Leonard Amaral ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Quorum Sensing Inhibition ◽  
Related Compounds

Widespread distribution of prophages signaling the potential for adaptability and pathogenicity evolution of Ralstonia solanacearum species complex

Genomics ◽  
2021 ◽  
Author(s):  
Osiel Silva Gonçalves ◽  
Flávia de Oliveira Souza ◽  
Fernanda Prieto Bruckner ◽  
Mateus Ferreira Santana ◽  
Poliane Alfenas-Zerbini
Keyword(s):  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Widespread Distribution

Disproof of the Proposed Structures of Bradyoxetin, a Putative Bradyrhizobium japonicum Signaling Molecule, and HMCP, a Putative Ralstonia solanacearum Quorum-Sensing Molecule

2021 ◽  
Vol 84 (2) ◽  
pp. 495-502
Author(s):  
Arata Yajima ◽  
Ryo Katsuta ◽  
Mikaho Shimura ◽  
Ayaka Yoshihara ◽  
Tatsuo Saito ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Ralstonia Solanacearum ◽  
Bradyrhizobium Japonicum ◽  
Signaling Molecule ◽  
Quorum Sensing Molecule

Molecular characterization and aggressiveness of the Ralstonia solanacearum species complex from Eucalyptus spp. in Brazil

2021 ◽  
Author(s):  
Rodrigo G. Freitas ◽  
Pollyane S. Hermenegildo ◽  
Lúcio M. S. Guimarães ◽  
Edival A. V. Zauza ◽  
Jorge L. Badel ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ralstonia Solanacearum ◽  
Species Complex
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