Making the Enterobacterial Common Antigen Glycan and Measuring Its Substrate Sequestration

2021 ◽  
Author(s):  
Colleen R. Eade ◽  
Timothy W. Wallen ◽  
Claire E. Gates ◽  
Cassidy L. Oliverio ◽  
Beth A. Scarbrough ◽  
...  
Keyword(s):  
Common Antigen ◽  
Enterobacterial Common Antigen

scholarly journals A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification

2021 ◽  
Vol 22 (2) ◽  
pp. 701
Author(s):  
Tomasz K Gozdziewicz ◽  
Anna Maciejewska ◽  
Alona Tsybulska ◽  
Czeslaw Lugowski ◽  
Jolanta Lukasiewicz
Keyword(s):  
Current Knowledge ◽  
Membrane Integrity ◽  
Gel Filtration ◽  
Common Antigen ◽  
Mild Acid Hydrolysis ◽  
Enterobacterial Common Antigen ◽  
Lipopolysaccharide Endotoxin ◽  
Lipid Moiety ◽  
Detailed Composition ◽  
Mild Acid

Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria. It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide − endotoxin (ECALPS), and cyclic form (ECACYC). ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria. A supernatant obtained after LPS ultracentrifugation was reported as a source for ECA isolation, but it has never been assessed for detailed composition besides ECACYC. We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations. Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants. We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation. Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units. Described results were common for all selected strains. The origin of linear ECA is discussed against the current knowledge about ECAPG and ECALPS.

scholarly journals Synthetic Enterobacterial Common Antigen (ECA) for the Development of a Universal Immunotherapy for Drug-ResistantEnterobacteriaceae

2015 ◽  
Vol 54 (37) ◽  
pp. 10953-10957 ◽  
Author(s):  
Lin Liu ◽  
Jingying Zha ◽  
Antonio DiGiandomenico ◽  
Douglas McAllister ◽  
C. Kendall Stover ◽  
...  
Keyword(s):  
Common Antigen ◽  
Enterobacterial Common Antigen

Antibody to Enterobacterial Common Antigen and Gram-Negative Sepsis

2004 ◽  
Vol 32 (2) ◽  
pp. 617
Author(s):  
Paul Bayardelle
Keyword(s):  
Common Antigen ◽  
Gram Negative ◽  
Enterobacterial Common Antigen

scholarly journals Enterobacterial common antigen.

1976 ◽  
Vol 40 (3) ◽  
pp. 591-632 ◽  
Author(s):  
P H Mäkelä ◽  
H Mayer
Keyword(s):  
Common Antigen ◽  
Enterobacterial Common Antigen

scholarly journals Serological and Immunological Properties of Isolated Enterobacterial Common Antigen

1978 ◽  
Vol 86 (2) ◽  
pp. 371-379 ◽  
Author(s):  
Daniela MANNEL ◽  
Hubert MAYER
Keyword(s):  
Common Antigen ◽  
Immunological Properties ◽  
Enterobacterial Common Antigen

scholarly journals Comparison of enterobacterial common antigen from different species by serological techniques

1987 ◽  
Vol 162 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Hella-Monika KUHN ◽  
Sumanta BASU ◽  
Hubert MAYER
Keyword(s):  
Common Antigen ◽  
Enterobacterial Common Antigen

scholarly journals Mutation in a Gene Required for Lipopolysaccharide and Enterobacterial Common Antigen Biosynthesis Affects Virulence in the Plant Pathogen Erwinia carotovora subsp. atroseptica

1999 ◽  
Vol 12 (6) ◽  
pp. 499-507 ◽  
Author(s):  
I. K. Toth ◽  
C. J. Thorpe ◽  
S. D. Bentley ◽  
V. Mulholland ◽  
L. J. Hyman ◽  
...  
Keyword(s):  
Surface Defects ◽  
Single Gene ◽  
Outer Membrane Proteins ◽  
Pectate Lyase ◽  
Erwinia Carotovora ◽  
Enteric Bacteria ◽  
Common Antigen ◽  
In Planta ◽  
Enterobacterial Common Antigen ◽  
Multiple Cell

Spontaneous bacteriophage-resistant mutants of the phytopathogen Erwinia carotovora subsp. atroseptica (Eca) SCRI1043 were isolated and, out of 40, two were found to exhibit reduced virulence in planta. One of these mutants, A5/22, showed multiple cell surface defects including alterations in synthesis of outer membrane proteins, lipopolysaccharide (LPS), enterobacterial common antigen (ECA), and flagella. Mutant A5/22 also showed reduced synthesis of the exoenzymes pectate lyase (Pel) and cellulase (Cel), major virulence factors for this pathogen. Genetic analysis revealed the pronounced pleiotropic mutant phenotype to be due to a defect in a single gene (rffG) that, in Escherichia coli, is involved in the production of ECA. We also show that while other enteric bacteria possess duplicate homologues of this gene dedicated separately to synthesis of LPS and ECA, Eca has a single gene.

Effect of erythrocytes treated with enterobacterial common antigen on experimentalSalmonella typhimurium infection of mice

1975 ◽  
Vol 161 (3) ◽  
pp. 163-170 ◽  
Author(s):  
Eugene A. Gorzynski ◽  
Susan A. Krasny
Keyword(s):  
Common Antigen ◽  
Enterobacterial Common Antigen
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