Enzyme-Linked Immunosorbent Assay for Pork Determination in Raw and Heated Meats: Combination of Monoclonal Antibodies to Denatured Porcine Myoglobin and Sodium Dodecyl Sulfate Extraction

The calculation of cleaning carryover limits in multi-product facilities can be based on the inactivity of molecules after exposure to cleaning conditions if the inactivation of active molecules can be demonstrated. The demonstration of inactivation has been addressed in several publications that have shown degradation and/or denaturation using different analytical techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis and enzyme-linked immunosorbent assay, which directly or indirectly demonstrate that the product residue is no longer active. In this paper, authors expand the assay options by demonstrating the use of molecule-specific cell-based activity assays, which provide a “catch all” measurement of sample bioactivity, to assess the inactivation of therapeutic antibodies after exposure to cleaning conditions.

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Using differential scanning calorimetry for the development of non-reduced capillary electrophoresis sodium dodecyl sulfate methods for monoclonal antibodies

Analytical Biochemistry ◽

10.1016/j.ab.2020.113948 ◽

2020 ◽

Vol 609 ◽

pp. 113948

Author(s):

Patricia Molina ◽

Arthur J. Schick ◽

Leslie Welch ◽

Thomas Niedringhaus ◽

Gabriella del Hierro ◽

...

Keyword(s):

Differential Scanning Calorimetry ◽

Capillary Electrophoresis ◽

Monoclonal Antibodies ◽

Sodium Dodecyl Sulfate ◽

Sodium Dodecyl ◽

Scanning Calorimetry ◽

Dodecyl Sulfate

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Screening of durum wheats for pasta-making quality with monoclonal antibodies for gliadin 45

Genome ◽

10.1139/g89-560 ◽

1989 ◽

Vol 32 (6) ◽

pp. 1096-1099 ◽

Cited By ~ 11

Author(s):

N. K. Howes ◽

M. I. Kovacs ◽

D. Leisle ◽

M. R. Dawood ◽

W. Bushuk

Keyword(s):

Monoclonal Antibodies ◽

Durum Wheat ◽

Immunosorbent Assay ◽

Sodium Dodecyl ◽

Wheat Breeding ◽

Rapid Screening ◽

Enzyme Linked Immunosorbent Assay ◽

Gluten Strength ◽

High Sodium ◽

Sedimentation Volume

Monoclonal antibodies specific for gliadin band 45 (gli 45) of common wheat cultivar 'Marquis' were treated against durum wheat gliadins using an enzyme-linked immunosorbent assay (ELISA). In a test of 15 durum cultivars, high ELISA values were associated with gli 45, high gluten strength, and high sodium dodecyl sulfate (SDS) sedimentation volume; low ELISA values were associated with gli 42, low gluten strength, and low SDS-sedimentation volume. Analysis of 120 F5 lines from the backcrosses Ward/Vic//Vic and Ward/Vic//Ward confirmed that a high reaction to ELISA was statistically correlated with the presence of gli 45, with high gluten strength, and with high SDS-sedimentation volume. It was concluded that monoclonal antibodies specific for gli 45 have potential as a test for rapid screening of durum wheat breeding populations for desirable pasta-making quality.Key words: monoclonal antibodies, gliadin, enzyme-linked immunosorbent assay, durum wheat.

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