Formulation and Pharmaceutical Assessment of Annona muricata Oral Capsules and Suspension as Antidiarrhea Dosage Forms

Annona muricata (Annonaceae) is a tropical plant widely known for its edible fruits. Recent scientific studies have confirmed the folkloric use of its seeds as an antidiarrheal agent. This study sought to formulate capsules and suspensions using the ethanolic extract from Annona muricata seeds. The dried ethanolic extract was formulated into granules and subsequently encapsulated. The suspension formulated was assessed for sedimentation rate, sedimentation volume, viscosity, dissolution, drug content, and flow rate, while pharmacopeia tests such as disintegration, dissolution, uniformity of weight, and drug content were carried out on the formulated capsules. The formulated suspension passed the drug content and in vitro release studies. Annona muricata suspension exhibited pseudoplastic flow with good sedimentation rate and sedimentation volume. The formulated capsules passed the in vitro dissolution studies, weight uniformity, disintegration, and drug content tests. The ethanolic extract of Annona muricata seeds was appropriately formulated into standardized solid and liquid oral dosage forms.

Sedimentation Rate Analysis in Dock C PT. Petrokimia Gresik, Indonesia

Dock C is one of the supporting facilities at PT. Petrokimia Gresik which functions as loading and unloading goods from ships to land and vice versa. Another benefit of the jetty is that it can significantly increase the company's production capacity. Dock C PT. Petrokimia Gresik. In the treatment of sedimentation in port it is necessary to analyze the current pattern and sedimentation rate early, because this is the first step to predict the amount of sedimentation contained in the port in a certain period of time. Several ways can be done to minimize the rate of sedimentation, one of which is the layout of Dock C to reduce the frequency of dredging. This study compares the sedimentation volume at the existing condition jetty and two alternative jetty that occur due to currents and waves, while modeling is done using Mike 21. The results of the existing jetty modeling show the sedimentation volume for 12 months was 20641.68 m3. Whereas the sedimentation volume from alternative jetty 1 and 2 produced for 12 months was 11293.56 m3 and 7426.2 m3. Modifications to the layout of the jetty provided were able to reduce the rate of sedimentation in Dock C, with the most optimal sedimentation volume for 12 months at 13215.48 m3

Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (Triticum aestivum L.)

Sodium dodecyl sulfate-sedimentation volume is an important index to evaluate the gluten strength of common wheat and is closely related to baking quality. In this study, a total of 15 quantitative trait locus (QTL) for sodium dodecyl sulfate (SDS)-sedimentation volume (SSV) were identified by using a high-density genetic map including 2,474 single-nucleotide polymorphism (SNP) markers, which was constructed with a doubled haploid (DH) population derived from the cross between Non-gda3753 (ND3753) and Liangxing99 (LX99). Importantly, four environmentally stable QTLs were detected on chromosomes 1A, 2D, and 5D, respectively. Among them, the one with the largest effect was identified on chromosome 1A (designated as QSsv.cau-1A.1) explaining up to 39.67% of the phenotypic variance. Subsequently, QSsv.cau-1A.1 was dissected into two QTLs named as QSsv.cau-1A.1.1 and QSsv.cau-1A.1.2 by saturating the genetic linkage map of the chromosome 1A. Interestedly, favorable alleles of these two loci were from different parents. Due to the favorable allele of QSsv.cau-1A.1.1 was from the high-value parents ND3753 and revealed higher genetic effect, which explained 25.07% of the phenotypic variation, mapping of this locus was conducted by using BC3F1 and BC3F2 populations. By comparing the CS reference sequence, the physical interval of QSsv.cau-1A.1.1 was delimited into 14.9 Mb, with 89 putative high-confidence annotated genes. SSVs of different recombinants between QSsv.cau-1A.1.1 and QSsv.cau-1A.1 detected from DH and BC3F2 populations showed that these two loci had an obvious additive effect, of which the combination of two favorable loci had the high SSV, whereas recombinants with unfavorable loci had the lowest. These results provide further insight into the genetic basis of SSV and QSsv.cau-1A.1.1 will be an ideal target for positional cloning and wheat breeding programs.

Characterization and purification of Algerian natural bentonite for pharmaceutical and cosmetic applications

Introduction Bentonitic clays from the Hammam Boughrara deposit in the Maghnia area (northwestern Algeria) were studied by mineralogical, chemical and physicochemical characterization to evaluate their potential suitability as raw and purified materials in pharmaceutical and cosmetic applications. Methodology Natural bentonite was purified by Na+ ion exchange treatment combined with sedimentation techniques. Before use in the pharmaceutical industry, bentonite samples must be safe and conform to recommendations and directives of pharmacopeia. A set of technological tests were investigated with the samples, such as cation exchange capacity (CEC), specific surface area (SSA), swelling capacity (SC),sedimentation volume (SV) and viscosity, and mineralogical, chemical and microbial properties were also identified by X-ray fluorescence (XRF), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Results Mineralogical data proved that the raw bentonite is mainly composed of smectite and illite with small quantities of gangue minerals such quartz, feldspars (orthoclase and albite) and calcite. The purified bentonite matches the mineralogical properties of Wyoming bentonite as an international standard clay (deposits of high economic value). Quartz and feldspars were successfully eliminated in the absence of illite and calcite after beneficiation. Investigation of chemical analyses indicated that the contents of trace elements (particularly Pb and As) were below the more restrictive limits proposed by major pharmacopeias for raw and purified bentonite clay. For microbiological tests, the absence of Escherichia coli, Salmonella species, Staphylococcus aureus and Pseudomonas aeruginosa was confirmed. Moreover, we note that a high cation exchange capacity, large surface area, and good swelling capacity and sedimentation volume were also obtained for purified bentonite. Conclusion In view of the fundamentals of major pharmacopoeias for the use of bentonite in pharmacies and considering the results obtained, we identified a pharmaceutically acceptable designation for purified Algerian bentonite, which can be used as a pharmaceutical excipient and in cosmetic products such as creams, powders and emulsions.

Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.

Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.

Reservoir sedimentation regime analysis: case study of Kedungombo reservoir and Sermo reservoir

The Kedungombo and the Sermo Reservoirs have problems in fulfilling basic services because of sedimentation. Sedimentation that occurs in each of the reservoirs would form a specific reservoir sedimentation pattern that is supposed to be similar because the hydrology and physiography conditions of the reservoir's catchment area are similar. This study aims to determine the dynamics of sedimentation patterns that occur in the dead storage for reviewing the characteristics/sedimentation regime of the two reservoirs. The analysis was carried out by processing bathymetrical data which were processed into a digital terrain model (DTM) using ArcGIS. Furthermore, the storage volume, sedimentation volume, storage percentage, and specific reservoir sedimentation rate are calculated. The results showed that the two reservoirs showed an increase in sedimentation volume each year so that the reservoir characteristic curve shifted from the plan graph. The dead storage capacity of Kedungombo Reservoir is 100% in 1989 to 43% in 2016 and 100% of Sermo Reservoir in 1997 to 58% in 2011. The specific reservoir sedimentation rate, i.e. 0.0031 and 0.0042 million m3/year/km2 for the Kedungombo Reservoir (between 1989 and 2016) and the Sermo Reservoir (between 1997 and 2011) respectively, indicating that the two reservoirs are in the same regime

Evaluation of Aloe elegans Mucilage as a Suspending Agent in Paracetamol Suspension

Background. There are various natural excipients which have been used as suspending agents in pharmaceutical suspensions due to the presence of mucilage in their specialized cells and their capacity to form a colloidal gel in an aqueous medium. Objective. The purpose of this study was to evaluate the suspending capacity of Aloe elegans mucilage in suspension formulations. Materials and Methods. Aloe elegans mucilage (AEM) was evaluated as a suspending agent in comparison with xanthan gum (XG) in paracetamol suspensions at 1, 2, 3, 4, and 5% ( w / v ) concentrations. The resulting suspensions were evaluated for their sedimentation volume, apparent viscosity, flow rate, rate of redispersibility, pH, assay, and dissolution profile. Results. The volume of sedimentation, apparent viscosity, and redispersibility rate of the formulations were significantly increased ( p < 0.05 ), with the concentration of the suspending agents. Meanwhile, the apparent viscosity for all formulations has significantly decreased ( p < 0.05 ) with an increase in shear rates. Volume of sedimentation, apparent viscosity, and redispersibility degree of the formulations prepared with AEM were significantly ( p < 0.05 ) lower than XG-containing formulations at the same concentration. Nevertheless, the sedimentation volume of all formulations with AEM was significantly ( p < 0.05 ) higher than the suspension without any suspending agent. With regard to drug content and pH values, all formulations showed an acceptable result with the standards. All formulations showed a release of greater than 85% of drug content within 45 min. Conclusion. Aloe elegans mucilage could have a potential to be utilized as an alternative suspending agent in pharmaceutical suspensions.

Formulation and In Vitro Evaluation of Oral Capsules and Suspension from the Ethanolic Extract of Cola nitida Seeds for the Treatment of Diarrhea

Management of diarrhea has evolved over the years from relatively inadequate interventions in the early years to more successful physiological approaches. The use of herbal medicinal products and supplements has grown significantly over the past three decades, with more than half of the global population depending on it for some aspect of their primary health care needs. This study is aimed at formulating solid and liquid oral dosage forms of the ethanolic extract of Cola nitida seeds for the treatment of diarrhea. The flow property of the dried ethanolic extract was determined and subsequently formulated into granules for encapsulation. The ethanolic extract was also used in formulating an oral suspension. Pharmacopeia tests such as uniformity of weight, disintegration, drug content, and dissolution were carried out on the formulated capsules. The formulated suspension was also assessed using the following parameters; viscosity, flow rate, drug content, dissolution, sedimentation rate, and sedimentation volume. The dried ethanolic extract and formulated granules exhibited good flow properties. The formulated capsules exhibited optimal in vitro release of extract (>90% after 45 minutes) and passed the uniformity of weight, disintegration, and drug content tests. The formulated suspension also passed the drug content test and had a good sedimentation rate, sedimentation volume, and flow rate. The formulated suspension also exhibited pseudoplastic flow, optimal viscosity, and a good in vitro release profile (>90% after 45 minutes). Capsules and suspension of the ethanolic extract of Cola nitida seeds have been successfully formulated and can be used as standard dosage forms for the management of diarrhea.

Purification and modification of neem gum for enhancement of its suspending property

Background The present study aimed to purify and modify the neem gum (NG) to evaluate its dispersing ability in a pharmaceutical suspension formulation. The modification was carried out to cross-link the sugars as carbamate in the presence of calcium chloride to improve the suspending property. Physiochemical properties such as pH, solubility, swelling index and ash value were performed before investigating the dispersing potential. The suspending potential of neem gum was studied in its different forms such as purified and modified gum in paracetamol suspension and was compared with sodium carboxymethylcellulose (CMC) being used as standard at a concentration range of 0.25–1% (w/v). The test suspensions were evaluated for the redispersibility, flowability, sedimentation volume (%) and stability study for 3 months. Result The redispersibility of modified neem gum (MNG) was found equal to CMC at a higher concentration. The flowability and apparent sedimentation of test suspending agents and CMC were found in the order of NG > MNG > CMC. It showed a positive correlation with the viscosity of suspension formulations. All the test paracetamol suspension formulations were found stable in the stability study. Conclusion The findings of the present study showed that as an alternate suspending agent, modified cross-linked neem gum could be used.

Evaluation of Aloe weloensis (Aloeacea) Mucilages as a Pharmaceutical Suspending Agent

Natural polymers, specifically mucilages, have been used as a suspending agent for a long period of time. Natural excipients can serve as an alternative to synthetic products since they are less expensive, less toxic, and devoid of environmental pollution. There are many species of Aloe found in Ethiopia which can be used as a source of mucilage. In this study, mucilage from Aloe weloensis, which is found in Wollo floristic region, was extracted and tested as a suspending agent at different suspending agent concentrations and compared with standard suspending agents (acacia and sodium carboxy methylcellulose (NaCMC)) by formulating zinc oxide suspension. The mucilage obtained from Aloe weloensis leaves has shown comparable suspending agent ability with acacia. The rate of sedimentation and viscosity was higher at 1% and 4% mucilage concentrations than acacia though the difference was not significant ( p > 0.05 ). The suspension was slightly basic and easily dispersible than NaCMC. Suspensions formulated from NaCMC were superior in terms of viscosity and sedimentation volume which was significantly different ( p < 0.05 ) accompanied by lower flow rates than suspensions formulated from acacia and Aloe weloensis mucilages. The results suggested that Aloe weloensis mucilage could be used as an alternative suspending agent.