scholarly journals Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification

ACS Omega ◽  
2020 ◽  
Vol 5 (50) ◽  
pp. 32738-32743
Author(s):  
Fubing Xiao ◽  
Jie Liu ◽  
Qinghui Guo ◽  
Zhibo Du ◽  
Hong Li ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Amplification Strategy ◽  
Dual Signal Amplification

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

G-quadruplex fluorescent probe-mediated real-time rolling circle amplification strategy for highly sensitive microRNA detection

2016 ◽  
Vol 943 ◽  
pp. 114-122 ◽  
Author(s):  
Hong-Xin Jiang ◽  
Zhen-Zhen Liang ◽  
Yan-Hong Ma ◽  
De-Ming Kong ◽  
Zhang-Yong Hong
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Amplification Strategy

A DNAzyme-mediated target-initiated rolling circle amplification strategy based on microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at single-cell level

2021 ◽  
Author(s):  
Chunhuan Xu ◽  
Shengyu Chen ◽  
Jingjin Zhao ◽  
Xiaoshu Luo ◽  
Shulin Zhao
Keyword(s):  
Single Cell ◽  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Single Cell Level ◽  
Cell Level ◽  
Rolling Circle ◽  
Amplification Strategy

A DNAzyme-mediated target-initiated rolling circle signal amplification strategy based on microchip platform was developed for detecting apurinic/apyrimidine endonuclease 1 (APE1) at the single-cell level. This strategy was applied for assay...

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

2014 ◽  
Vol 50 (13) ◽  
pp. 1576-1578 ◽  
Author(s):  
Liang Cui ◽  
Zhi Zhu ◽  
Ninghang Lin ◽  
Huimin Zhang ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Amplification Method ◽  
Microrna Detection ◽  
Dual Amplification ◽  
Amplification Strategy ◽  
Excellent Selectivity ◽  
T7 Exonuclease

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

scholarly journals A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection

2010 ◽  
Vol 38 (15) ◽  
pp. e156-e156 ◽  
Author(s):  
Yuntao Zhou ◽  
Qing Huang ◽  
Jimin Gao ◽  
Jianxin Lu ◽  
Xizhong Shen ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Amplification Strategy

A cascade signal amplification strategy for ultrasensitive colorimetric detection of BRCA1 gene

RSC Advances ◽  
2015 ◽  
Vol 5 (35) ◽  
pp. 27571-27575 ◽  
Author(s):  
Dong Liang ◽  
Wei You ◽  
Yang Yu ◽  
Yao Geng ◽  
Feng Lv ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Colorimetric Detection ◽  
Brca1 Gene ◽  
Gene Detection ◽  
Rolling Circle ◽  
Amplification Strategy

Schematic illustration of a colorimetric biosensor for breast cancer1 gene detection based on DNAzyme assistant DNA recycling and rolling circle amplification.

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