A cascade signal amplification strategy for ultrasensitive colorimetric detection of BRCA1 gene

RSC Advances ◽  
2015 ◽  
Vol 5 (35) ◽  
pp. 27571-27575 ◽  
Author(s):  
Dong Liang ◽  
Wei You ◽  
Yang Yu ◽  
Yao Geng ◽  
Feng Lv ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Colorimetric Detection ◽  
Brca1 Gene ◽  
Gene Detection ◽  
Rolling Circle ◽  
Amplification Strategy

Schematic illustration of a colorimetric biosensor for breast cancer1 gene detection based on DNAzyme assistant DNA recycling and rolling circle amplification.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

A DNAzyme-mediated target-initiated rolling circle amplification strategy based on microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at single-cell level

2021 ◽  
Author(s):  
Chunhuan Xu ◽  
Shengyu Chen ◽  
Jingjin Zhao ◽  
Xiaoshu Luo ◽  
Shulin Zhao
Keyword(s):  
Single Cell ◽  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Single Cell Level ◽  
Cell Level ◽  
Rolling Circle ◽  
Amplification Strategy

A DNAzyme-mediated target-initiated rolling circle signal amplification strategy based on microchip platform was developed for detecting apurinic/apyrimidine endonuclease 1 (APE1) at the single-cell level. This strategy was applied for assay...

A cascade amplification strategy based on rolling circle amplification and hydroxylamine amplified gold nanoparticles enables chemiluminescence detection of adenosine triphosphate

The Analyst ◽  
2014 ◽  
Vol 139 (15) ◽  
pp. 3796-3803 ◽  
Author(s):  
Ping Wang ◽  
Tonghuan Zhang ◽  
Taoyi Yang ◽  
Nan Jin ◽  
Yanjun Zhao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Adenosine Triphosphate ◽  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Enzymatic Reaction ◽  
Chemiluminescence Detection ◽  
Rolling Circle ◽  
Amplification Strategy ◽  
Cascade Amplification

A chemiluminescent biosensor for ATP was developed by taking advantage of the ATP-dependent enzymatic reaction, the powerful signal amplification capability of rolling circle amplification, and hydroxylamine-amplified gold nanoparticles.

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