A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

2014 ◽  
Vol 50 (13) ◽  
pp. 1576-1578 ◽  
Author(s):  
Liang Cui ◽  
Zhi Zhu ◽  
Ninghang Lin ◽  
Huimin Zhang ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Amplification Method ◽  
Microrna Detection ◽  
Dual Amplification ◽  
Amplification Strategy ◽  
Excellent Selectivity ◽  
T7 Exonuclease

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

G-quadruplex fluorescent probe-mediated real-time rolling circle amplification strategy for highly sensitive microRNA detection

2016 ◽  
Vol 943 ◽  
pp. 114-122 ◽  
Author(s):  
Hong-Xin Jiang ◽  
Zhen-Zhen Liang ◽  
Yan-Hong Ma ◽  
De-Ming Kong ◽  
Zhang-Yong Hong
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Amplification Strategy

scholarly journals A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection

2010 ◽  
Vol 38 (15) ◽  
pp. e156-e156 ◽  
Author(s):  
Yuntao Zhou ◽  
Qing Huang ◽  
Jimin Gao ◽  
Jianxin Lu ◽  
Xizhong Shen ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Amplification Strategy

A T7 exonuclease-RCA dual amplification system for high-sensitivity and high-selectivity analysis of microRNA

2019 ◽  
Vol 11 (18) ◽  
pp. 2450-2455 ◽  
Author(s):  
Yan Xu ◽  
Yan Li ◽  
Hongkun Zhou ◽  
Xiaoping Mei ◽  
Jieyun Ye ◽  
...  
Keyword(s):  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Selective Detection ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Amplification System ◽  
Dual Amplification ◽  
T7 Exonuclease

A novel combination of rolling circle amplification and T7 exonuclease-assisted amplification to achieve highly sensitive and selective detection of microRNA molecules was developed.

A novel DNA biosensor for ultrasensitive detection of DNA methyltransferase activity based on a high-density “hot spots” SERS substrate and rolling circle amplification strategy

The Analyst ◽  
2021 ◽  
Author(s):  
Shengjie Ge ◽  
Menglin Ran ◽  
Yu Mao ◽  
Yue Sun ◽  
Xinyu Zhou ◽  
...  
Keyword(s):  
Hot Spots ◽  
Dna Methyltransferase ◽  
Rolling Circle Amplification ◽  
High Density ◽  
Dna Biosensor ◽  
Ultrasensitive Detection ◽  
Sers Substrate ◽  
Methyltransferase Activity ◽  
Rolling Circle ◽  
Amplification Strategy

Herein, we proposed a novel biosensor based on the high-density “hot spots” Au@SiO2 array substrate and rolling circle amplification (RCA) strategy for ultrasensitive detection of the CpG methyltransferase (M.SssI) activity....

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4180-4187 ◽  
Author(s):  
Krissana Khoothiam ◽  
Kiatnida Treerattrakoon ◽  
Tawin Iempridee ◽  
Patraporn Luksirikul ◽  
Tararaj Dharakul ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Graphene Oxide ◽  
Cancer Diagnosis ◽  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle

MPRCA-GO sensor can detect low amount of miRNAs, which are biomarkers in cancer diagnosis.

CRISPR-Cas12a enhanced rolling circle amplification method for ultrasensitive miRNA detection

2020 ◽  
Vol 158 ◽  
pp. 105239
Author(s):  
Gong Zhang ◽  
Lei Zhang ◽  
Jingtao Tong ◽  
Xianxian Zhao ◽  
Jianlin Ren
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Amplification Method ◽  
Mirna Detection
Sign in / Sign up

Export Citation Format

Share Document