Controlling Peptide Function by Directed Assembly Formation: Mechanistic Insights Using Multiscale Modeling on an Antimicrobial Peptide–Drug–Membrane System

ABSTRACT The presence of the cationic phospholipid lysyl-phosphatidylglycerol (lysyl-PG) in staphylococcal cytoplasmic membranes has been linked to increased resistance to cationic compounds, including antibiotics such as daptomycin as well as host defense antimicrobial peptides. We investigated the effects of lysyl-PG on binding of 6W-RP-1, a synthetic antimicrobial peptide, to lipid vesicles and on peptide-induced membrane permeabilization. Unexpectedly, physiological lysyl-PG concentrations only minimally reduced membrane binding of 6W-RP-1. In contrast, 6W-RP-1-induced dye leakage was severely inhibited by lysyl-PG, suggesting that lysyl-PG primarily impacts membrane defect formation.

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Construction of antimicrobial peptide-drug combination networks from scientific literature based on a semi-automated curation workflow

Database ◽

10.1093/database/baw143 ◽

2016 ◽

Vol 2016 ◽

pp. baw143 ◽

Cited By ~ 4

Author(s):

Paula Jorge ◽

Martín Pérez-Pérez ◽

Gael Pérez Rodríguez ◽

Florentino Fdez-Riverola ◽

Maria Olívia Pereira ◽

...

Keyword(s):

Antimicrobial Peptide ◽

Drug Combination ◽

Scientific Literature ◽

Peptide Drug

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Lactoferrin-Derived Antimicrobial Peptide Induces a Micellar Cubic Phase in a Model Membrane System

Biophysical Journal ◽

10.1016/j.bpj.2011.06.038 ◽

2011 ◽

Vol 101 (3) ◽

pp. L20-L22 ◽

Cited By ~ 16

Author(s):

Margarida Bastos ◽

Tânia Silva ◽

Vitor Teixeira ◽

Kamran Nazmi ◽

Jan G.M. Bolscher ◽

...

Keyword(s):

Antimicrobial Peptide ◽

Membrane System ◽

Model Membrane ◽

Cubic Phase

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A Freeze-Etch Study of Acinetobacter SP. Grown on a Hydrocarbon Substrate

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050883 ◽

1974 ◽

Vol 32 ◽

pp. 174-175

Author(s):

C. L. Scott ◽

W. R. Finnerty

Keyword(s):

Membrane System ◽

Structural Distortion ◽

Intracytoplasmic Membrane ◽

Gram Negative ◽

Sectioned Material ◽

Acinetobacter Sp

Acinetobacter sp. HO-1-N, a gram-negative hydrocarbon oxidizing bacterium previously designated Micrococcus cerificans, has been shown to sequester the hydrocarbon into intracytoplasmic pools as a result of growth on this substrate. In hydrocarbon grown cells, an intracytoplasmic membrane system was also observed along with a doubling of cellular phospholipids (Z). However, using conventional dehydration and embedding procedures in preparing thin sectioned material, the hydrocarbon is extracted from the cells. This may lead to structural distortion, consequently, the freeze-etch technique was applied to preserve the integrity of the cell.

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A potassium permanganate fixative for membranes in sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161175 ◽

1990 ◽

Vol 48 (3) ◽

pp. 722-723

Author(s):

Jindan Song

Keyword(s):

Potassium Permanganate ◽

Cultured Cells ◽

Calf Serum ◽

Trisodium Citrate ◽

Membrane System ◽

Adenocarcinoma Cell Line ◽

Mouse Embryo Fibroblast ◽

African Green Monkey Kidney ◽

Adenocarcinoma Cell ◽

Green Monkey

Potassium permanganate has been used as a fixative for the botanical specimen and membrane system in thin section by Glauert (1975). A new potassium permanganate fixative ( Trisodium citrate 60mM, Potassium chloride 25mM, Magnesium chloride 35mM, and Potassium permanganate 125mM ) for localizing membranous system in whole_mount cultured cells with standard trasmission electron microscopy and phase_contrast microscopy has been developed). Here, we report that using this new potassium permanganate fixative for membranous system in sections.Cultured cells, CV_1 (African green monkey kidney epithelial cells), Balb/c 3T3 ( Mouse embryo fibroblast ) and MCF_7 (Human adenocarcinoma cell line) were used for this study. All cells were grown on 35mm plastic dishes in DME medium containing 5% calf serum at 37 c with 100% humidity and 5% CO2. Using the potassium permanganate fixative to fix the cells for about 7 minutes. After fixation, the cells were dehydrated in a graded series of ethanol.

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