A potassium permanganate fixative for membranes in sections

1990 ◽  
Vol 48 (3) ◽  
pp. 722-723
Author(s):  
Jindan Song
Keyword(s):  
Potassium Permanganate ◽  
Cultured Cells ◽  
Calf Serum ◽  
Trisodium Citrate ◽  
Membrane System ◽  
Adenocarcinoma Cell Line ◽  
Mouse Embryo Fibroblast ◽  
African Green Monkey Kidney ◽  
Adenocarcinoma Cell ◽  
Green Monkey

Potassium permanganate has been used as a fixative for the botanical specimen and membrane system in thin section by Glauert (1975). A new potassium permanganate fixative ( Trisodium citrate 60mM, Potassium chloride 25mM, Magnesium chloride 35mM, and Potassium permanganate 125mM ) for localizing membranous system in whole_mount cultured cells with standard trasmission electron microscopy and phase_contrast microscopy has been developed). Here, we report that using this new potassium permanganate fixative for membranous system in sections.Cultured cells, CV_1 (African green monkey kidney epithelial cells), Balb/c 3T3 ( Mouse embryo fibroblast ) and MCF_7 (Human adenocarcinoma cell line) were used for this study. All cells were grown on 35mm plastic dishes in DME medium containing 5% calf serum at 37 c with 100% humidity and 5% CO2. Using the potassium permanganate fixative to fix the cells for about 7 minutes. After fixation, the cells were dehydrated in a graded series of ethanol.

scholarly journals The T84 human colonic adenocarcinoma cell line produces mucin in culture and releases it in response to various secretagogues

1990 ◽  
Vol 267 (2) ◽  
pp. 491-500 ◽  
Author(s):  
D J McCool ◽  
M A Marcon ◽  
J F Forstner ◽  
G G Forstner
Keyword(s):  
Cell Line ◽  
Cultured Cells ◽  
Solid Phase ◽  
Secretory Granules ◽  
Chloride Secretion ◽  
Colonic Adenocarcinoma ◽  
Mucin Secretion ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Mucin Release

The T84 colonic adenocarcinoma cell line, which has been used extensively as a model for studies of epithelial chloride secretion, also produces mucin and secretes it in culture. Electron microscopy of fixed sections of cultured cells, along with Immunogold labelling with an antibody to human small intestine (SI) mucin, revealed the presence of goblet-like cells with mucin-containing secretory granules. The mucin was of high molecular mass, had an amino acid composition similar to that of purified human SI and colonic mucins, and competed effectively with SI mucin for binding to the anti-(SI mucin) antibody. A sensitive solid-phase immunoassay specific for intestinal mucins was developed and used to measure mucin secretion by T84 cells. Cultures were treated for 30 min at 37 degrees C with a number of agents known to cause chloride secretion by T84 cell monolayers and the amount of mucin appearing in the medium was measured. Carbachol (1 mM), A23187 (10 microM), prostaglandin E1 (PGE1) (1 microM) and vasoactive intestinal polypeptide (VIP) (0.1 microM) all stimulated mucin release, but histamine (1 mM) had no effect. Whereas VIP is reported to stimulate chloride secretion more strongly than carbachol, it was less effective than carbachol in stimulating mucin secretion. Phorbol 12-myristate 13-acetate (PMA) (0.1-10 microM) also stimulated mucin release strongly, implicating a responsive protein-kinase C-dependent pathway. Additive secretory responses were obtained with combined stimulation by VIP (10 nM-1 microM) and carbachol (1 mM). Responses to stimulation with A23187 (1-10 microM) together with PMA (10 nM-10 microM) suggest that cytosolic Ca2+ concentration is a modulator of PMA activity.

scholarly journals Effect of vitex agnus castus fruits methanol extract against murine mammary adenocarcinoma cell line (amn3) and rat embryonic fibroblast normal cell line (ref)

2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Zeinat. W. Ribat ◽  
Hayder B Sahib ◽  
Amjed Haseeb Khamees ◽  
Ahmed M. Al-Shammari
Keyword(s):  
Cell Line ◽  
Methanol Extract ◽  
Calf Serum ◽  
Fibroblast Cell ◽  
Mouse Mammary Gland ◽  
Mammary Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Agnus Castus ◽  
Embryonic Fibroblast

Vitex agnus castus showed antiproliferative activity in several previous studies. Angiogenesis is one of the targets in the remediation of cancer. This study aimed to demonstrate the effect of methanol fruits extract of vitex agnus castus on mouse mammary gland adenocarcinoma cell line and rat embryonic fibroblast cell line.  The cell lines used in this study were obtained from tissue culture unit/ Iraqi Center for Cancer and Medical Genetic Researches, Al-Mustansiriyah University was maintained in RPMI- 1640 tissue media after preparing from 10% fetal calf serum, antibiotics solution and other materials to make complete growth medium. Serial solutions of vitex agnus castus, methanol crude extract have been tested on 10*4 of AMN3 and REF in each well of 96 well plates. The results of the current study showed that the concentration that inhibits fifty percent of cell line after 72 hours of the experiment (IC50) was 129 ug/ml for AMN3 and 1324ug/ml for REF cell line. The antioxidant activity of Vitex agnus castus may indicate the proliferation inhibition activity of Vitex agnus castus methanol extract. The study concluded that this extract might be of benefit if used in combination with other anti-cancer drugs as adjuvant therapy.

Effects of growth factors EGF and TGF-β in the endometrial adenocarcinoma cell line HEC 1B

1989 ◽  
Vol 120 (3_Suppl) ◽  
pp. S236 ◽  
Author(s):  
T. STROWITZKI ◽  
M. WIMBAUER ◽  
D. PFEIFFER ◽  
P. SCHEIDEL
Keyword(s):  
Growth Factors ◽  
Cell Line ◽  
Endometrial Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell

Synthesis, Anti-proliferative Evaluation, and Molecular Docking Studies of 3-(alkylthio)-5,6-diaryl-1,2,4-triazines as Tubulin Polymerization Inhibitors

2019 ◽  
Vol 16 (11) ◽  
pp. 1194-1201 ◽  
Author(s):  
Farhad Saravani ◽  
Ebrahim Saeedian Moghadam ◽  
Hafezeh Salehabadi ◽  
Seyednasser Ostad ◽  
Morteza Pirali Hamedani ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Cytotoxic Activity ◽  
Binding Site ◽  
Cell Line ◽  
Cell Lines ◽  
Tubulin Polymerization ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Colchicine Binding Site ◽  
Anti Cancer

Background: The role of microtubules in cell division and signaling, intercellular transport, and mitosis has been well known. Hence, they have been targeted for several anti-cancer drugs. Methods: A series of 3-(alkylthio)-5,6-diphenyl-1,2,4-triazines were prepared and evaluated for their cytotoxic activities in vitro against three human cancer cell lines; human colon carcinoma cells HT-29, human breast adenocarcinoma cell line MCF-7, human Caucasian gastric adenocarcinoma cell line AGS as well as fibroblast cell line NIH-3T3 by MTT assay. Docking simulation was performed to insert these compounds into the crystal structure of tubulin at the colchicine binding site to determine a probable binding model. Compound 5d as the most active compound was selected for studying of microtubule disruption. Results: Compound 5d showed potent cytotoxic activity against all cell lines. The molecular modeling study revealed that some derivatives of triazine strongly bind to colchicine binding site. The tubulin polymerization assay kit showed that the cytotoxic activity of 5d may be related to inhibition of tubulin polymerization. Conclusion: The cytotoxicity and molecular modeling study of the synthesized compounds with their inhibition activity in tubulin polymerization demonstrate the potential of triazine derivatives for development of new anti-cancer agents.

scholarly journals Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 114
Author(s):  
Mohammed Bourhia ◽  
Kaoutar Bouothmany ◽  
Hanane Bakrim ◽  
Safa Hadrach ◽  
Ahmad Mohammad Salamatullah ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Cell Line ◽  
Antiproliferative Activity ◽  
Seed Extract ◽  
Diffusion Method ◽  
Citrullus Colocynthis ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Ht 29 ◽  
Β Carotene

Background: Citrullus colocynthis L. (C. colocynthis) is commonly known as colocynth. It belongs to the family Cucurbitaceae that is frequently used in alternative medicine in the north of Africa. The aim of the study: the present research was undertaken to investigate the chemical composition, antioxidant, antiproliferative, and antibacterial potentials of C. colocynthis seed extract. Material and methods: the chemical composition of C. colocynthis seed organic extract was characterized using gas chromatography/mass spectrometry (GC-MS). The antioxidant property was carried out using both β-carotene bleaching and DPPH assays. The antibacterial effect was effectuated using the agar disc diffusion method. The antiproliferative activity vs. human colorectal adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MDA MB 231) were carried by WST-1 test. The chemical analysis showed the presence of interesting potentially bioactive compounds. The studied plant extract exhibited antioxidant potential with IC50 value of 2. 22 mg/mL (β-carotene bleaching) and 8.98 ± 0.619 mg/mL (DPPH). Concerning the antiproliferative activity, the seed extract was effective in MDA-MB-231 and HT-29 cancer cells with IC50 values 86.89 ± 3.395 and 242.1 ± 17.9 μg/mL, respectively, whilst the extract of Citrullus colocynthis seeds was non-toxic in healthy human dermal fibroblasts. Regarding the antibacterial test, the extract was effective in Gram-positive bacteria only. Conclusion: The outcome of this research indicated that the extracts from C. colocynthis seeds may compose a promising source with interesting compounds that can be used to fight cancer, free radicals damage, and bacterial infections.

5-Fluorouracil and its active metabolite FdUMP cause DNA damage in human SW620 colon adenocarcinoma cell line

2009 ◽  
Vol 29 (4) ◽  
pp. 308-316 ◽  
Author(s):  
Renata Matuo ◽  
Fabrício Garmus Sousa ◽  
Alexandre E. Escargueil ◽  
Ivana Grivicich ◽  
Daniel Garcia-Santos ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Line ◽  
Active Metabolite ◽  
Colon Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell
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