A Freeze-Etch Study of Acinetobacter SP. Grown on a Hydrocarbon Substrate

1974 ◽  
Vol 32 ◽  
pp. 174-175
Author(s):  
C. L. Scott ◽  
W. R. Finnerty
Keyword(s):  
Membrane System ◽  
Structural Distortion ◽  
Intracytoplasmic Membrane ◽  
Gram Negative ◽  
Sectioned Material ◽  
Acinetobacter Sp

Acinetobacter sp. HO-1-N, a gram-negative hydrocarbon oxidizing bacterium previously designated Micrococcus cerificans, has been shown to sequester the hydrocarbon into intracytoplasmic pools as a result of growth on this substrate. In hydrocarbon grown cells, an intracytoplasmic membrane system was also observed along with a doubling of cellular phospholipids (Z). However, using conventional dehydration and embedding procedures in preparing thin sectioned material, the hydrocarbon is extracted from the cells. This may lead to structural distortion, consequently, the freeze-etch technique was applied to preserve the integrity of the cell.

Intracytoplasmic Membrane Formation in the Hydrocarbon Oxidizing Bacterium, Micrococcus Cerificans

1971 ◽  
Vol 29 ◽  
pp. 264-265
Author(s):  
R. S. Kennedy ◽  
Ben O. Spurlock ◽  
W. R. Finnerty
Keyword(s):  
Cytoplasmic Membrane ◽  
Membrane System ◽  
Sole Source ◽  
Defined Medium ◽  
Intracellular Membrane ◽  
Intracytoplasmic Membrane ◽  
Logarithmic Growth Phase ◽  
Gram Negative ◽  
Defined Media ◽  
Logarithmic Growth

Micrococcus cerificans is a gram negative, pleomorphic, coccoid bacterium capable of growth in complex or chemically defined media. The complex medium employed was nutrient broth-yeast extract (NBYE) while hydrocarbons constituted the sole source of carbon and energy in the chemically defined medium. Cells cultured in the above media were harvested in the late logarithmic growth phase and fixed according to the procedure of Kellenberger, et al. A variety of embedding techniques were used including those developed by Luft; Staubli; and Spurlock et al.M cerificans cultured in the NBYE medium showed characteristic gram negative morphology including mesosomes. In the hydrocarbon grown cells an intracellular membrane system distinct from mesosomes was observed. Evidence suggests that these highly ordered membranous structures which appear as sheaths or bundles extending the width and/or length of the cells are continuous with the cytoplasmic membrane and are specific to hydrocarbon grown cells.

Methanogenesis in the absence of intracytoplasmic membranes

1984 ◽  
Vol 30 (5) ◽  
pp. 594-604 ◽  
Author(s):  
G. D. Sprott ◽  
L. C. Sowden ◽  
J. R. Colvin ◽  
K. F. Jarrell ◽  
T. J. Beveridge
Keyword(s):  
Membrane System ◽  
Accurate Method ◽  
Methanobacterium Thermoautotrophicum ◽  
Methane Formation ◽  
Bacterial Cells ◽  
Optimal Temperature ◽  
Intracytoplasmic Membrane ◽  
Methanospirillum Hungatei ◽  
Large Numbers ◽  
Intracytoplasmic Membranes

The frequency of intracytoplasmic membranes in several methanogens grown on H2–CO2 varied with the conditions of growth and varied from one strain to another. Methanobacterium thermoautotrophicum often generated large numbers of intracytoplasmic membranes, while Methanospirillum hungatei produced these membranes only rarely. Conditions allowing for rapid growth, including optimal temperature and high agitation rates, increased the production of intracytoplasmic membranes. These membranes consisted mainly of vesicles composed of one or several membrane layers, often positioned in the central region of the cytoplasm. Several mesophilic methanogens could be grown such that intracytoplasmic membranes were rarely or never observed in thin section or in replicas of cross-fractures from frozen cells. Since high rates of methane synthesis still occurred in these cultures, it follows that the intracytoplasmic membrane system is not a necessary organelle for methane formation in these strains. Negative staining for electron microscopy is not an accurate method to visualize intracytoplasmic membranes in these bacterial cells.

scholarly journals Methylomagnum ishizawai gen. nov., sp. nov., a mesophilic type I methanotroph isolated from rice rhizosphere

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3527-3534 ◽  
Author(s):  
Ashraf Khalifa ◽  
Chol Gyu Lee ◽  
Takuya Ogiso ◽  
Chihoko Ueno ◽  
Dayéri Dianou ◽  
...  
Keyword(s):  
Type Strain ◽  
Carbon Assimilation ◽  
Membrane System ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Type I ◽  
Intracytoplasmic Membrane ◽  
Respiratory Quinone ◽  
Rice Rhizosphere ◽  
The Family

An aerobic, methane-oxidizing bacterium (strain RS11D-PrT) was isolated from rice rhizosphere. Cells of strain RS11D-PrT were Gram-stain-negative, motile rods with a single polar flagellum and contained an intracytoplasmic membrane system typical of type I methanotrophs. The strain utilized methane and methanol as sole carbon and energy sources. It could grow at 20–37 °C (optimum 31–33 °C), at pH 6.8–7.4 (range 5.5–9.0) and with 0–0.2 % (w/v) NaCl (there was no growth at above 0.5 % NaCl). pmoA and mmoX genes were present. The ribulose monophosphate and/or ribulose bisphosphate pathways were used for carbon assimilation. Results of sequence analysis of 16S rRNA genes showed that strain RS11D-PrT is related closely to the genera Methylococcus, Methylocaldum, Methyloparacoccus and Methylogaea in the family Methylococcaceae. The similarity was low (94.6 %) between strain RS11D-PrT and the most closely related type strain (Methyloparacoccus murrellii R-49797T). The DNA G+C content was 64.1 mol%. Results of phylogenetic analysis of the pmoA gene and chemotaxonomic data regarding the major cellular fatty acids (C16 : 1ω7c, C16 : 0 and C14 : 0) and the major respiratory quinone (MQ-8) also indicated the affiliation of strain RS11D-PrT to the Methylococcus–Methylocaldum–Methyloparacoccus–Methylogaea clade. On the basis of phenotypic, genotypic and phylogenetic characteristics, strain RS11D-PrT is considered to represent a novel genus and species within the family Methylococcaceae, for which the name Methylomagnum ishizawai gen. nov., sp. nov. is proposed. The type strain is RS11D-PrT ( = JCM 18894T = NBRC 109438T = DSM 29768T = KCTC 4681T).

scholarly journals Identifikasi Bakteri dan Faktor Risiko Kejadian Pressure Ulcer di RSUD AWS Samarinda

2019 ◽  
Vol 4 (7) ◽  
pp. 391
Author(s):  
Diah Setiani ◽  
Indah Nur Imamah
Keyword(s):  
Pressure Ulcer ◽  
Gram Negative ◽  
Acinetobacter Sp ◽  
Stroke Center

Adanya pressure ulcer mengganggu proses pemulihan pasien, diikuti komplikasi dengan nyeri dan infeksi sehingga menambah panjang lama perawatan. Pressure ulcer menimbulkan sebuah ancaman dalam pelayanan kesehatan karena insidennya semakin hari semakin meningkat (Spilsbury, et al, 2010). Tujuan penelitian ini, mengetahui gambaran bakteri pada pressure ulcer dan faktor  kejadian pressure ulcer di RSUD AWS Samarinda. Metode penelitian yang digunakan adalah penelitian deskriptif. Dimana populasi adalah semua pasien yang mengalami pressure ulcer. Teknik pengambilan sampel (sampling) menggunakan metode accidental sampling dilaksanakan di RSUD AWS Samarinda pada bulan Oktober Tahun 2017 meliputi ruang HCU, stroke center, anggrek, angsoka dan flamboyan sebanyak 11 orang.  Variable dalam yang diukur penelitian ini adalah identifikasi bakteri, faktor risiko (persepsi sensori, kelembaban, aktifitas, mobilisasi, nutrisi, gesekan dan robekan).  Analisis yang digunakan adalah analisis univariat yaitu dalam bentuk data yang disajikan dalam bentuk tabel distribusi frekuensi dan persentase, meliputi karakteristik responden dan faktor risiko skala braden.  Penelitian ini telah mendapatkan persetujuan kelakayan etik dari komisi etik penelitian kesehatan RSUD A. Wahab Sjahranie Samarinda dengan nomor 030/KEPK-AWS/XI/2017 . Hasil penelitian yang dilakukan pada 11 orang responden didapatkan: Jenis kuman gram negative paling banyak terdapat di pressure ulcer sebesar (81 %) Acinetobacter sp adalah mikroorganisme yang paling dominan terdapat diluka pressure, sebanyak 63,6 % responden  memiliki persepsi dan sensori normal, sebanyak 63,6 % kelembaban responden adalah selalu basah, sebanyak 81,8% kegiatan pasien pressure ulcer adalah bedrest, sebanyak 63,6% mobilitas responden adalah imobilisasi total, sebanyak 54,5% kondisi nutrisi responden adalah cukup, sebanyak 72,7% gesekan dan robekan reponden memiliki masalah. Bagi para pemberi pelayanan diharapkan dapat melakukan pengkajian faktor kejadian pressure ulcer serta dapat mengendalikan infeksi pada pasien dengan ketergantungan total/ bedrest sehingga angka kejadian dapat berkurang.

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