Synthesis of High-Molecular Weight Biobased Epoxy Resins: Determination of the Course of the Process by MALDI-TOF Mass Spectrometry

2018 ◽  
Vol 6 (5) ◽  
pp. 6084-6093 ◽  
Author(s):  
Anna Sienkiewicz ◽  
Piotr Czub
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Epoxy Resins ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

Qualitative determination of early Maillard-products by MALDI-TOF mass spectrometry peptide mapping

2002 ◽  
Vol 215 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Thomas Kislinger ◽  
Andreas Humeny ◽  
Silke Seeber ◽  
Cord-Michael Becker ◽  
Monika Pischetsrieder
Keyword(s):  
Mass Spectrometry ◽  
Peptide Mapping ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Maillard Products ◽  
Qualitative Determination

scholarly journals Determination of the δ 2H Values of High Molecular Weight Lipids by High Temperature GC Coupled to Isotope Ratio Mass Spectrometry

2020 ◽  
Author(s):  
Sabine Lengger ◽  
Yuki Weber ◽  
Kyle W. Taylor ◽  
Sebastian H. Kopf ◽  
Robert Berstan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
High Temperature ◽  
High Molecular Weight ◽  
Isotope Ratio ◽  
Isotope Ratio Mass Spectrometry ◽  
Widespread Interest ◽  
Isotope Analyses

<div>Rationale: The hydrogen isotopic composition of lipids (δ 2Hlipid) is widely used in food</div><div>science and as a proxy for past hydrological conditions. Determining the δ 2H values of large,</div><div>well-preserved triacylglycerides and other uniquely microbial lipids, such as glycerol dialkyl</div><div>glycerol tetraether (GDGT) lipids, is thus of widespread interest but has so far not been </div><div>possible due to their size which prohibits analysis by traditional gas chromatography</div><div>pyrolysis isotope ratio mass spectrometry (GC-P-IRMS).</div><div>Methods: We determined the δ 2H values of large, polar molecules and applied high</div><div>temperature gas chromatography (GC) methods on a modified GC-P-IRMS system. The</div><div>methods were validated using authentic standards of large, functionalised molecules</div><div>(triacylglycerides, TAG), purified reference standards of GDGTs, and compared to δ 2H</div><div>values determined by elemental analyser pyrolysis isotope ratio mass spectrometry (EA-PIRMS); and subsequently applied to the analysis of GDGTs in a sample from a methane</div><div>seep and a Welsh peat.</div><div>Results: δ 2H values of TAGs agreed within error between different between GC-P-IRMS and</div><div>EA-P-IRMS, with GC-P-IRMS showing 3-5 ‰ precision for 10 ng H injected. Archaeal lipid</div><div>GDGTs with up to three cyclisations could be analysed: δ 2H values were not significantly</div><div>different between methods with standard deviations of 5 to 6 ‰. When environmental</div><div>samples were analysed, δ 38 2H values of isoGDGTs were 50 ‰ more negative than those of</div><div>terrestrial brGDGTs.</div><div>Conclusions: Our results indicate that the high temperature GC-P-IRMS (HTGC-P-IRMS)</div><div>method developed here is appropriate to determine the δ 2H values of TAGs, GDGT lipids</div><div>with up to two cyclisations, and potentially other high molecular weight compounds. The</div><div>methodology will widen the current analytical window for biomarker and alimentary light</div><div>stable isotope analyses. Moreover, our initial measurements suggest that bacterial and</div><div>archaeal GDGT δ 2H values can record environmental and ecological conditions</div>

Determination of sensitivity of microorganisms of the Klebsiella genus to antimicrobial drugs by the MALDI-TOF MS method

Bacteriology ◽  
2020 ◽  
Vol 5 (3) ◽  
pp. 8-13
Author(s):  
A.A. Samoilova ◽  
◽  
I.V. Likhachev ◽  
E.V. Zueva ◽  
E.V. Rogacheva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antibiotic Resistance ◽  
Mass Spectra ◽  
Antimicrobial Agents ◽  
Antimicrobial Drugs ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Resistance Markers ◽  
Klebsiella Spp

Actual microbiological diagnostics of infections caused by Klebsiella spp. should include isolation of the strain, its identification and fastest possible determination of the pathogen susceptibility to antimicrobial agents. We evaluated the prospects of using MALDI-TOF mass spectrometry to determine the susceptibility of Klebsiella spp. strains to antimicrobial agents. According to the results of mass spectrometry Klebsiella spp. strains analysis, we carried out cluster analysis by the UPGMA method based on mass spectra and data on the susceptibility of the studied strains to antimicrobial agents, and then studied the obtained dendrograms. We identified the areas with the highest probability of the location of antibiotic resistance markers by comparing the mass spectra of susceptible and resistant microorganisms at different concentrations of antimicrobial agents. Thus, using MALDI-TOF mass spectrometry, a new direction in assessing the susceptibility of Klebsiella spp. to antimicrobial agents can be formed. Key words: antibiotic resistance, Klebsiella spp., MALDI-TOF mass spectrometry

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