scholarly journals A Multireporter Bacterial 2-Hybrid Assay for the High-Throughput and Dynamic Assay of PDZ Domain–Peptide Interactions

2019 ◽  
Vol 8 (5) ◽  
pp. 918-928 ◽  
Author(s):  
David M. Ichikawa ◽  
Carles Corbi-Verge ◽  
Michael J. Shen ◽  
Jamie Snider ◽  
Victoria Wong ◽  
...  
Keyword(s):  
High Throughput ◽  
Pdz Domain ◽  
Peptide Interactions ◽  
Dynamic Assay ◽  
Domain Peptide

Crystallographic Studies of PDZ Domain–Peptide Interactions of the Scribble Polarity Module

2021 ◽  
pp. 125-135
Author(s):  
Janesha C. Maddumage ◽  
Bryce Z. Stewart ◽  
Patrick O. Humbert ◽  
Marc Kvansakul
Keyword(s):  
Pdz Domain ◽  
Peptide Interactions ◽  
Domain Peptide

scholarly journals Erratum: Corrigendum: Predicting PDZ domain–peptide interactions from primary sequences

2008 ◽  
Vol 26 (10) ◽  
pp. 1193-1193
Author(s):  
Jiunn R Chen ◽  
Bryan H Chang ◽  
John E Allen ◽  
Michael A Stiffler ◽  
Gavin MacBeath
Keyword(s):  
Pdz Domain ◽  
Peptide Interactions ◽  
Domain Peptide

scholarly journals Simultaneous prediction of binding free energy and specificity for PDZ domain–peptide interactions

2013 ◽  
Vol 27 (12) ◽  
pp. 1051-1065 ◽  
Author(s):  
Joseph J. Crivelli ◽  
Gordon Lemmon ◽  
Kristian W. Kaufmann ◽  
Jens Meiler
Keyword(s):  
Free Energy ◽  
Binding Free Energy ◽  
Pdz Domain ◽  
Peptide Interactions ◽  
Simultaneous Prediction ◽  
Domain Peptide

scholarly journals A physical model for PDZ-domain/peptide interactions

2010 ◽  
Vol 17 (2) ◽  
pp. 315-324 ◽  
Author(s):  
Kristian Kaufmann ◽  
Nicole Shen ◽  
Laura Mizoue ◽  
Jens Meiler
Keyword(s):  
Physical Model ◽  
Pdz Domain ◽  
Peptide Interactions ◽  
Domain Peptide

scholarly journals Predicting PDZ domain–peptide interactions from primary sequences

2008 ◽  
Vol 26 (9) ◽  
pp. 1041-1045 ◽  
Author(s):  
Jiunn R Chen ◽  
Bryan H Chang ◽  
John E Allen ◽  
Michael A Stiffler ◽  
Gavin MacBeath
Keyword(s):  
Pdz Domain ◽  
Peptide Interactions ◽  
Domain Peptide

A Quantitative Fluorescence-Based Assay for Assessing LIM Domain-Peptide Interactions

2016 ◽  
Vol 55 (42) ◽  
pp. 13236-13239 ◽  
Author(s):  
Neil O. Robertson ◽  
Manan Shah ◽  
Jacqueline M. Matthews
Keyword(s):  
Lim Domain ◽  
Peptide Interactions ◽  
Domain Peptide ◽  
Quantitative Fluorescence

scholarly journals Semi-Supervised Prediction of SH2-Peptide Interactions from Imbalanced High-Throughput Data

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62732 ◽  
Author(s):  
Kousik Kundu ◽  
Fabrizio Costa ◽  
Michael Huber ◽  
Michael Reth ◽  
Rolf Backofen
Keyword(s):  
High Throughput ◽  
High Throughput Data ◽  
Peptide Interactions

scholarly journals A High-Throughput Screening Platform Targeting PDLIM5 for Pulmonary Hypertension

2016 ◽  
Vol 21 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Han Cheng ◽  
Tianji Chen ◽  
Merve Tor ◽  
Deborah Park ◽  
Qiyuan Zhou ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Drug Discovery ◽  
Cell Line ◽  
High Throughput ◽  
High Throughput Screening ◽  
Pdz Domain ◽  
Luciferase Reporter ◽  
Targeted Drug ◽  
Screening Platform ◽  
Targeted Drug Discovery

Pulmonary arterial hypertension is a complex disease with multiple etiologic factors. PDLIM5, a member of the Enigma subfamily of PDZ and LIM domain protein family, contains an N-terminal PDZ domain and three LIM domains at its C-terminus. We have previously shown that overexpression of PDLIM5 prevents hypoxia-induced pulmonary hypertension (PH), and deletion of PDLIM5 in smooth muscle cells enhances hypoxia-induced PH in vivo. These results suggest that PDLIM5 may be a novel therapeutic target of PH. In this study, we aim to establish a high-throughput screening platform for PDLIM5-targeted drug discovery. We generated a stable mink lung epithelial cell line (MLEC) containing a transforming growth factor–β/Smad luciferase reporter with lentivirus-mediated suppression of PDLIM5 (MLEC-shPDLIM5) and measured levels of Smad2/3 and pSmad2/3. We found that in MLEC, suppression of PDLIM5 decreased Smad-dependent luciferase activity, Smad3, and pSmad3. We used MLEC-shPDLIM5 and a control cell line (MLEC-shCTL) to screen the Prestwick library (1200 compounds) and identified and validated paclitaxel as a PDLIM5 inhibitor in MLEC. Furthermore, we showed that paclitaxel inhibited Smad2 expression and Smad3 phosphorylation in A549 cells. Our study suggests that this system is robust and suitable for PDLIM5-targeted drug discovery.

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