The effects of acetic, propionic, butyric and lactic acids (short-chain acids), and of glycerol, on the in vitro dry matter digestibility (IVDMD) of oat hay, and on cellulolysis by a protein extract of rumen fluid were studied. The objective was to gain information on the impact of increasing the concentrations of those compounds in the medium on degradative activities carried out by rumen microorganisms. Short-chain acids were assayed as sodium salts and, for all compounds, the concentrations were 50, 100, 200 and 300 mM. The volatile fatty acids (VFA: acetic, propionic and butyric acids) were tested separately or in two mixtures A and B that contained different proportions of each VFA. The IVDMD was assayed according to a modified Tilley & Terry (1963) technique while cellulolysis was assessed by the hydrolysis of carboxymethylcellulose (CMC) in 1-h incubations at 39 °C. Parallel incubations with NaCl were utilized as controls for ionic strength and osmolarity changes in the incubation medium. Increases of 100–300 mM of short-chain acids decreased IVDMD between 7 and 39%. The inhibitions produced by increases of 200 and 300 mM of acetic acid were smaller than those caused by the same concentrations of either butyric or lactic acids. Increases of 100, 200 and 300 mM of glycerol reduced by 8–15% the IVDMD, and the inhibitions produced by increments of 200 and 300 mM of this compound were smaller than the ones elicited by the short-chain acids, with the exception of 200 mM of acetic acid that did not differ. The IVDMD was inhibited by NaCl only when concentrations were increased by 200 (12%) and 300 mM (26%). The degradation of CMC was reduced by 7–20% by 200 and 300 mM increases of the short-chain acids or glycerol, the exception being lactic acid that had no effect. One hundred mM increases of acetic and butyric acids inhibited the CMC degradation by 7 and 9%, respectively, whereas only butyric acid was inhibitory (7%) at 50 mM. Greater concentration increases of both VFA mixtures A and B than of the individual VFA were necessary to inhibit the hydrolysis of CMC. Cellulolytic activity was decreased 6 and 9% by increases of 200 and 300 mM of NaCl, respectively, and these drops of activity were smaller than those obtained with increases in any of the other compounds. Since osmolarity and/or ionic strength changes in the medium cannot completely account for the observed inhibitions of IVDMD and cellulolysis, it is suggested that glycerol and the anions of short-chain acids produce changes in the reaction media polarity that contribute to the inhibitory effects observed. Alterations in the media could also bring about conformational changes in the degradative enzymes leading to reduced rates of reaction and subsequent decreases in IVDMD and cellulolysis. Since most of the effects were observed with concentration increases that were larger than the physiological concentrations in the rumen, it is suggested that the compounds tested have little impact in vivo on the ruminal degradative activities that were evaluated.
Iodination of nucleosomes at low ionic strength: conformational changes in H4 and stabilization by H1
