Substrate Translocation Kinetics of Excitatory Amino Acid Carrier 1 Probed with Laser-Pulse Photolysis of a New Photolabile Precursor ofd-Aspartic Acid†

Biochemistry ◽  
2001 ◽  
Vol 40 (1) ◽  
pp. 232-240 ◽  
Author(s):  
Christof Grewer ◽  
Sayed Abdollah Madani Mobarekeh ◽  
Natalie Watzke ◽  
Thomas Rauen ◽  
Klaus Schaper
Keyword(s):  
Amino Acid ◽  
Laser Pulse ◽  
Aspartic Acid ◽  
Excitatory Amino Acid ◽  
Pulse Photolysis ◽  
Laser Pulse Photolysis ◽  
Kinetics Of ◽  
Amino Acid Carrier

Reaction kinetics of the intermediate produced in the laser pulse photolysis of tungsten hexacarbonyl in fluid solution

1981 ◽  
Vol 20 (12) ◽  
pp. 4381-4384 ◽  
Author(s):  
Alistair J. Lees ◽  
Arthur W. Adamson
Keyword(s):  
Laser Pulse ◽  
Reaction Kinetics ◽  
Fluid Solution ◽  
Pulse Photolysis ◽  
Tungsten Hexacarbonyl ◽  
Laser Pulse Photolysis ◽  
Kinetics Of

Kinetics of smooth and skeletal muscle activation by laser pulse photolysis of caged inositol 1,4,5-trisphosphate

Nature ◽  
1987 ◽  
Vol 327 (6119) ◽  
pp. 249-252 ◽  
Author(s):  
J. W. Walker ◽  
A. V. Somlyo ◽  
Y. E. Goldman ◽  
A. P. Somlyo ◽  
D. R. Trentham
Keyword(s):  
Skeletal Muscle ◽  
Laser Pulse ◽  
Muscle Activation ◽  
Pulse Photolysis ◽  
Laser Pulse Photolysis ◽  
Inositol 1,4,5 Trisphosphate ◽  
Kinetics Of

scholarly journals Early Intermediates in the Transport Cycle of the Neuronal Excitatory Amino Acid Carrier Eaac1

2001 ◽  
Vol 117 (6) ◽  
pp. 547-562 ◽  
Author(s):  
Natalie Watzke ◽  
Ernst Bamberg ◽  
Christof Grewer
Keyword(s):  
Amino Acid ◽  
Binding Sites ◽  
Excitatory Amino Acid ◽  
Sodium Ion ◽  
Ion Concentration ◽  
Charge Movement ◽  
Pulse Photolysis ◽  
Transport Cycle ◽  
Charge Movements ◽  
Amino Acid Carrier

Electrogenic glutamate transport by the excitatory amino acid carrier 1 (EAAC1) is associated with multiple charge movements across the membrane that take place on time scales ranging from microseconds to milliseconds. The molecular nature of these charge movements is poorly understood at present and, therefore, was studied in this report in detail by using the technique of laser-pulse photolysis of caged glutamate providing a 100-μs time resolution. In the inward transport mode, the deactivation of the transient component of the glutamate-induced coupled transport current exhibits two exponential components. Similar results were obtained when restricting EAAC1 to Na+ translocation steps by removing potassium, thus, demonstrating (1) that substrate translocation of EAAC1 is coupled to inward movement of positive charge and, therefore, electrogenic; and (2) the existence of at least two distinct intermediates in the Na+-binding and glutamate translocation limb of the EAAC1 transport cycle. Together with the determination of the sodium ion concentration and voltage dependence of the two-exponential charge movement and of the steady-state EAAC1 properties, we developed a kinetic model that is based on sequential binding of Na+ and glutamate to their extracellular binding sites on EAAC1 explaining our results. In this model, at least one Na+ ion and thereafter glutamate rapidly bind to the transporter initiating a slower, electroneutral structural change that makes EAAC1 competent for further, voltage-dependent binding of additional sodium ion(s). Once the fully loaded EAAC1 complex is formed, it can undergo a much slower, electrogenic translocation reaction to expose the substrate and ion binding sites to the cytoplasm.

Channel-Opening Kinetics of GluR2QflipAMPA Receptor:  A Laser−Pulse Photolysis Study†

Biochemistry ◽  
2003 ◽  
Vol 42 (42) ◽  
pp. 12358-12366 ◽  
Author(s):  
Gang Li ◽  
Weimin Pei ◽  
Li Niu
Keyword(s):  
Laser Pulse ◽  
Pulse Photolysis ◽  
Channel Opening ◽  
Laser Pulse Photolysis ◽  
Kinetics Of

scholarly journals Expression of Jejunal Apical Excitatory Amino Acid Carrier 1 (EAAC1) is Maintained in Piglets with Bowel Inflammation

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Chengbo Yang ◽  
Dale Lackeyram ◽  
Tania Archbold ◽  
Yoshinori Mine ◽  
Ming Fan
Keyword(s):  
Amino Acid ◽  
Excitatory Amino Acid ◽  
Amino Acid Carrier

scholarly journals Multiple Signaling Pathways Regulate Cell Surface Expression and Activity of the Excitatory Amino Acid Carrier 1 Subtype of Glu Transporter in C6 Glioma

1998 ◽  
Vol 18 (7) ◽  
pp. 2475-2485 ◽  
Author(s):  
Karen E. Davis ◽  
Dean J. Straff ◽  
Edward A. Weinstein ◽  
Peter G. Bannerman ◽  
Dana M. Correale ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Surface ◽  
Signaling Pathways ◽  
Excitatory Amino Acid ◽  
Surface Expression ◽  
C6 Glioma ◽  
Cell Surface Expression ◽  
Multiple Signaling ◽  
Amino Acid Carrier ◽  
Expression And Activity

The role of diurnal fluctuations in excitatory amino acid carrier 1 levels in post-ischemic hippocampal Zn2+ accumulation

2021 ◽  
Vol 336 ◽  
pp. 113538
Author(s):  
Takaaki Aratake ◽  
Youichirou Higashi ◽  
Tomoya Hamada ◽  
Yusuke Ueba ◽  
Takahiro Shimizu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Excitatory Amino Acid ◽  
Diurnal Fluctuations ◽  
Amino Acid Carrier
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