Studies of protein-protein association between yeast cytochrome c peroxidase and yeast iso-1 ferricytochrome c by hydrogen-deuterium exchange labeling and proton NMR spectroscopy

Biochemistry ◽  
1994 ◽  
Vol 33 (40) ◽  
pp. 12032-12041 ◽  
Author(s):  
Qian Yi ◽  
James E. Erman ◽  
James D. Satterlee
Keyword(s):  
Nmr Spectroscopy ◽  
Cytochrome C ◽  
Proton Nmr ◽  
Cytochrome C Peroxidase ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Proton Nmr Spectroscopy ◽  
Protein Association ◽  
Ferricytochrome C ◽  
Hydrogen Deuterium

scholarly journals The Effect of the Asn82Asp Mutation in Yeast Cytochrome c Peroxidase Studied by Proton NMR Spectroscopy

1994 ◽  
Vol 224 (1) ◽  
pp. 81-87 ◽  
Author(s):  
James D. Satterlee ◽  
Steve L. Alam ◽  
J. Matthew Mauro ◽  
James E. Erman ◽  
Thomas L. Poulos
Keyword(s):  
Nmr Spectroscopy ◽  
Cytochrome C ◽  
Proton Nmr ◽  
Cytochrome C Peroxidase ◽  
Proton Nmr Spectroscopy

Determination of the accessible hydroxyl groups in heat-treated Styrax tonkinensis (Pierre) Craib ex Hartwich wood by hydrogen-deuterium exchange and 2H NMR spectroscopy

Holzforschung ◽  
2007 ◽  
Vol 61 (5) ◽  
pp. 488-491 ◽  
Author(s):  
Le Xuan Phuong ◽  
Masato Takayama ◽  
Satoshi Shida ◽  
Yuji Matsumoto ◽  
Tetsuo Aoyagi
Keyword(s):  
Nmr Spectroscopy ◽  
Treated Wood ◽  
Deuterium Exchange ◽  
Fungal Resistance ◽  
Lower Amount ◽  
Hydroxyl Groups ◽  
Hydrogen Deuterium Exchange ◽  
Oh Groups ◽  
Heat Treated ◽  
Hydrogen Deuterium

Abstract A new approach based on hydrogen-deuterium exchange is proposed for measuring accessible OH groups in wood. The deuterium (D) exchanged for hydrogen in OH groups in wood was converted to D2O by combustion in oxygen gas then diluted in deionized water, and subsequently determined by 2H NMR spectroscopy. The amount of accessible OH groups in Styrax tonkinensis wood is approximately 6.8 mmol g-1. This measurement is very accurate, with an error of approximately 0.2 mmol g-1. Heat-treated wood has a lower amount of accessible OH groups than non-treated wood. This finding is in agreement with the decreased hygroscopicity of heat-treated wood and explains, at least partially, its increased fungal resistance.

Identification of the barstar binding site of barnase by NMR spectroscopy and hydrogen-deuterium exchange

FEBS Letters ◽  
1993 ◽  
Vol 331 (1-2) ◽  
pp. 165-172 ◽  
Author(s):  
David N.M. Jones ◽  
Mark Bycroft ◽  
Michael J. Lubienski ◽  
Alan R. Fersht
Keyword(s):  
Nmr Spectroscopy ◽  
Binding Site ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Hydrogen Deuterium

Proton NMR spectroscopy of Paracoccus denitrificans cytochrome c-550

Biochemistry ◽  
1984 ◽  
Vol 23 (15) ◽  
pp. 3526-3533 ◽  
Author(s):  
Russell Timkovich ◽  
Margaret S. Cork ◽  
Priscilla V. Taylor
Keyword(s):  
Nmr Spectroscopy ◽  
Cytochrome C ◽  
Paracoccus Denitrificans ◽  
Proton Nmr ◽  
Proton Nmr Spectroscopy
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