Structural determination of the capsular polysaccharide of Neisseria meningitidis group I: a two-dimensional NMR analysis

Biochemistry ◽  
1985 ◽  
Vol 24 (20) ◽  
pp. 5592-5598 ◽  
Author(s):  
Francis Michon ◽  
Jean Robert Brisson ◽  
Rene Roy ◽  
Fraser E. Ashton ◽  
Harold J. Jennings
Keyword(s):  
Neisseria Meningitidis ◽  
Capsular Polysaccharide ◽  
Structural Determination ◽  
Two Dimensional ◽  
Nmr Analysis ◽  
Group I

ChemInform Abstract: SYNTHESIS AND STRUCTURAL DETERMINATION OF A NEW TWO-DIMENSIONAL COMPOUND: P2NB4S21

1983 ◽  
Vol 14 (34) ◽  
Author(s):  
R. BREC ◽  
M. EVAIN ◽  
P. GRENOUILLEAU ◽  
J. ROUXEL
Keyword(s):  
Structural Determination ◽  
Two Dimensional

Structural determination of the capsular polysaccharide of Streptococcus pneumoniae type 19A (57)

1983 ◽  
Vol 124 (2) ◽  
pp. 235-245 ◽  
Author(s):  
Ewa Katzenellenbogen ◽  
Harold J. Jennings
Keyword(s):  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Structural Determination

Structural determination of geometrical isomers of microcystins LR and RR from cyanobacteria by two-dimensional NMR spectroscopic techniques

1990 ◽  
Vol 3 (5) ◽  
pp. 473-481 ◽  
Author(s):  
Kenichi Harada ◽  
Kiyoshi Ogawa ◽  
Kenji Matsuura ◽  
Hideaki Murata ◽  
Makoto Suzuki ◽  
...  
Keyword(s):  
Spectroscopic Techniques ◽  
Structural Determination ◽  
Two Dimensional ◽  
Geometrical Isomers

Structural determination of the capsular polysaccharide of Streptococcuspneumoniae type-19 (19F)

1980 ◽  
Vol 58 (11) ◽  
pp. 1069-1074 ◽  
Author(s):  
Harold J. Jennings ◽  
Karl-Gunnar Rosell ◽  
Dennis J. Carlo
Keyword(s):  
Nmr Spectroscopy ◽  
Capsular Polysaccharide ◽  
Molar Ratio ◽  
Structural Determination ◽  
Pneumococcus Type

The structure of the Pneumococcus type-19 (19F) capsular polysaccharide has been reinvestigated using nmr spectroscopy. It is composed of residues of 2-acetamido-2-deoxy-D-mannose, D-glucose, L-rhamnose, and phosphate in the molar ratio of 1:1:1:1. The polysaccharide is linear and is composed of the above components in a repeating unit of the following structure:→ 4)-β-D-ManNAcp-(1 → 4)-α-D-Glcp-(1→ 2)-α-L-Rhap-(1-PO4−)→.

Structural determination of the O-antigenic polysaccharide of enteropathogenicEscherichia coliO103:H2

2010 ◽  
Vol 56 (5) ◽  
pp. 367-372 ◽  
Author(s):  
Evgeny Vinogradov ◽  
Leann L. MacLean ◽  
Malcolm B. Perry
Keyword(s):  
Periodate Oxidation ◽  
Enterohemorrhagic Escherichia Coli ◽  
Structural Determination ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Polysaccharide Antigen ◽  
Antigenic Polysaccharide ◽  
Oxidation Degradation ◽  
C Nmr

The structure of the antigenic O-polysaccharide isolated from the lipopolysaccharide produced by enterohemorrhagic Escherichia coli O103:H2 was determined and shown to be composed of d-glucose (1 part), 2-acetamido-2-deoxy-d-glucose (2 parts), 2-acetamido-2-deoxy-d-galactose (1 part), and 3-deoxy-3-(R)-3-hydroxybutyramido-d-fucose (1 part). From the results of methylation analysis, Smith-type periodate oxidation degradation studies, and the use of one- and two-dimensional1H and13C NMR spectroscopy, the O-polysaccharide antigen was found to be an unbranched polymer of a repeating pentasaccharide unit having the following structure: →2)-β-d-Glcp-(1→2)-β-d-Fucp3NBu-(1→6)-α-d-GlcpNAc-(1→4)-α-d-GalpNAc-(1→3)-β-d-GlcpNAc-(1→, where Bu is (R)-3-hydroxybutyramido.

Structural determination of oligosaccharides derived from lipooligosaccharide of Neisseria gonorrhoeae F62 by chemical, enzymic, and two-dimensional NMR methods [Erratum to document cited in CA115(21):229924v]

Biochemistry ◽  
1992 ◽  
Vol 31 (1) ◽  
pp. 316-316
Author(s):  
Ryohei Yamasaki ◽  
Bradley E. Bacon ◽  
Wade Nasholds ◽  
Herman Schneider ◽  
J. M. Griffiss
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Structural Determination ◽  
Two Dimensional ◽  
Nmr Methods

scholarly journals STRUCTURAL DETERMINATION OF CYCLOALIPHATIC EPOXY MONOMERS BY TWO-DIMENSIONAL NMR SPECTROSCOPY AND QUANTUM CHEMICAL CALCULATION

1991 ◽  
Vol 7 (Supple) ◽  
pp. 425-428 ◽  
Author(s):  
ATSUSHI UDAGAWA ◽  
YASUHIKO YAMAMOTO ◽  
YOSHIO INOUE ◽  
RIICHIRO CHUJO
Keyword(s):  
Nmr Spectroscopy ◽  
Quantum Chemical Calculation ◽  
Quantum Chemical ◽  
Chemical Calculation ◽  
Structural Determination ◽  
Two Dimensional ◽  
Cycloaliphatic Epoxy

scholarly journals Investigation of bioluminescence-based assays for determination of kinetic parameters for the bifunctional Neisseria meningitidis serogroup W capsule polymerase

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Laleh Sheikhi Moghaddam ◽  
Ayobami Adegbite ◽  
Pumtiwitt C. McCarthy
Keyword(s):  
Sialic Acid ◽  
Neisseria Meningitidis ◽  
High Throughput ◽  
Capsular Polysaccharide ◽  
Vaccine Development ◽  
Kinetic Studies ◽  
Polymerase Activity ◽  
Control Activity ◽  
Free Nucleotides

Abstract Objective Neisseria meningitidis is a Gram-negative bacterium that causes meningitis. N. meningitidis serogroup W (NmW) capsule polymerase synthesizes capsular polysaccharide of this serogroup. This enzyme could be a tool for meningococcal glycoconjugate vaccine development. Our long-term goal is to control activity of the NmW capsule polymerase for production of defined carbohydrates for vaccines. The enzyme lacks a simple, high-throughput activity assay. Here, we describe the use of high-throughput bioluminescence assays (CMP-Glo and UDP-Glo by Promega) to investigate NmW capsule polymerase activity. These assays detect free nucleotides produced during transfer of sugar from UDP-Galactose and CMP-Sialic Acid to an acceptor. Kinetic studies using NmW hydrolyzed polysaccharide (PS) acceptor are described as well as preliminary work with a sialic acid trimer (DP3) acceptor. Results In CMP-Glo kinetic studies, with constant donor (80 µM) and varied NmW hydrolyzed polysaccharide (0–2000 µg/mL), a Km of 629.2 ± 101.4 µg/mL and a Vmax of 0.8965 ± 0.05823 µM/min was obtained. Using UDP-Glo, Km and Vmax values of 13.84 ± 9.675 µM and 0.6205 ± 0.1331 µM/min were obtained with varied CMP-NeuNAc (0–80 µM) and constant acceptor (400 µg/mL) and UDP-Gal (80 µM). This is the first report of using bioluminescence assays for NmW kinetics.

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