A differential scanning calorimetric study of the thermotropic phase behavior of model membranes composed of phosphatidylcholines containing linear saturated fatty acyl chains

Biochemistry ◽  
1987 ◽  
Vol 26 (19) ◽  
pp. 6118-6126 ◽  
Author(s):  
Ruthven N. A. H. Lewis ◽  
Nanette Mak ◽  
Ronald N. McElhaney
Keyword(s):  
Phase Behavior ◽  
Fatty Acyl ◽  
Model Membranes ◽  
Differential Scanning Calorimetric ◽  
Calorimetric Study ◽  
Acyl Chains

Temperature-induced changes in lecithin model membranes detected by novel covalent spin-labelled phospholipids

1977 ◽  
Vol 55 (2) ◽  
pp. 173-185 ◽  
Author(s):  
L. Stuhne-Sekalec ◽  
N. Z. Stanacev
Keyword(s):  
Transition Temperature ◽  
Fatty Acyl ◽  
Model Membranes ◽  
Head Group ◽  
Polar Head Group ◽  
Polar Group ◽  
Conformational Isomers ◽  
Hydrocarbon Chains ◽  
Acyl Chains ◽  
Induced Changes

Several spin-labelled phospholipids carrying covalently bound 5-doxylstearic acid (2-(3-carboxydecyl)-2-hexyl-4,4-dimethyl-3-oxazolidinoxyl) were intercalated in liposomes of saturated and unsaturated lecithins. Temperature-induced changes of these liposomes, detected by the spin-labelled phospholipids, were found to be in agreement with the previously described transitions of hydrocarbon chains of host lecithins detected by different probes and different techniques, establishing that spin-labelled phosopholipids are sensitive probes for the detection of temperature-induced changes in lecithin model membranes. In addition to the detection of already-known transitions in lecithin liposomes, the coexistence of two distinctly different environments was observed above the characteristic transition temperature. This phenomenon was tentatively attributed to the influence of the lecithin polar group on the fluidity of fatty acyl chains near the polar group. Combined with other results from the literature, the coexistence of two environments could be associated with the coexistence of two conformational isomers of lecithin, differing in the orientation of the polar head group with respect to the plane of bilayer. These findings have been discussed in view of the present state of knowledge regarding temperature-induced changes in model membranes.

Differential scanning calorimetric study of the thermotropic phase behavior of a polymerizable, tubule-forming lipid

1988 ◽  
Vol 48 (3-4) ◽  
pp. 215-230 ◽  
Author(s):  
Thomas G. Burke ◽  
Alan S. Rudolph ◽  
Ronald R. Price ◽  
James P. Sheridan ◽  
Adam W. Dalziel ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Differential Scanning Calorimetric ◽  
Calorimetric Study
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