Structural studies of .alpha.-bungarotoxin. 2. Proton NMR assignments via an improved relayed coherence transfer nuclear Overhauser enhancement experiment

Biochemistry ◽  
1988 ◽  
Vol 27 (8) ◽  
pp. 2772-2775 ◽  
Author(s):  
Vladimir J. Basus ◽  
Ruud M. Scheek
Keyword(s):  
Nmr Assignments ◽  
Proton Nmr ◽  
Structural Studies ◽  
Coherence Transfer ◽  
Nuclear Overhauser Enhancement ◽  
Nuclear Overhauser ◽  
Alpha Bungarotoxin

Structural studies on the polysaccharide portion of "A-band" lipopolysaccharide from a mutant (AK1401) of Pseudomonas aeruginosa strain PAO1

1991 ◽  
Vol 69 (8) ◽  
pp. 1273-1280 ◽  
Author(s):  
Todd L. Arsenault ◽  
Donald W. Hughes ◽  
David B. MacLean ◽  
Walter A. Szarek ◽  
Andrew M. B. Kropinski ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Structural Studies ◽  
Coherence Transfer ◽  
Strain Pao1 ◽  
Nmr Spectrum ◽  
H Nmr ◽  
Main Structural Feature ◽  
Nuclear Magnetic Resonance Spectra ◽  
Structure Formula ◽  
Nuclear Overhauser

AK1401 is a mutant of Pseudomonas aeruginosa strain PAO1 (serotype 05) that does not express O-antigen, but does express "A-band" lipopolysaccharide (LPS). The polysaccharide portion of the A-band LPS (A-PS) from AK1401 was found to consist mainly of D-rhamnose, with smaller amounts of 3-O-methylrhamnose, ribose, mannose, glucose, and a 3-O-methylhexose. 1H nuclear magnetic resonance spectra of the intact A-PS indicated that the main structural feature was a repeating trisaccharide of α-D-rhamnose having the following structure:[Formula: see text]The 1H NMR spectrum of the repeating unit was completely assigned through the use of 2D shift-correlated and relayed coherence transfer NMR spectroscopy. The linkage positions and sequence of residues were found by nuclear Overhauser enhancement difference spectroscopy. Key words: Pseudomonas aeruginosa, lipopolysaccharide, 1H NMR.

Structural studies of .alpha.-bungarotoxin. 1. Sequence-specific proton NMR resonance assignments

Biochemistry ◽  
1988 ◽  
Vol 27 (8) ◽  
pp. 2763-2771 ◽  
Author(s):  
Vladimir J. Basus ◽  
Martin Billeter ◽  
Robert A. Love ◽  
Robert M. Stroud ◽  
Irwin D. Kuntz
Keyword(s):  
Proton Nmr ◽  
Resonance Assignments ◽  
Nmr Resonance Assignments ◽  
Structural Studies ◽  
Alpha Bungarotoxin

The synthesis of 8-aza-3-deazaguanosine [6-amino-1-(β-D-ribofuranosyl)-υ-triazolo[4,5-c]pyridin-4-one] via a novel 1,3-dipolar cycloaddition reaction

1980 ◽  
Vol 58 (23) ◽  
pp. 2550-2561 ◽  
Author(s):  
Robert A. Earl ◽  
Leroy B. Townsend
Keyword(s):  
Spectral Data ◽  
Good Yield ◽  
Liquid Ammonia ◽  
Functional Group ◽  
Proton Nmr ◽  
Cycloaddition Reaction ◽  
Chemical Degradation ◽  
Dipolar Cycloaddition ◽  
Nuclear Overhauser Enhancement ◽  
Nuclear Overhauser

8-Aza-3-deazaguanosine (2) bas been prepared via a route which used a 1,3-dipolar cycloaddition reaction to provide a key intermediate. The reaction of 2′,3′,5′-tri-O-benzoyl-β-D-ribofuranosyl azide (13) with methyl 4-hydroxy-2-butynoate (11) provided a good yield of crystalline methyl 5-hydroxymethyl-1-(2,3,5-tri-O-benzoyl-β-D-ribofuranosyl)-υ-triazole-4-carboxylate (14). A series of functional group transformations were then used to convert 14 into methyl 5-cyanomethyl-1-(2,3,5-tri-O-benzoyl-β-D-ribofuranosyl)-υ-triazolo-4-carboxylate (19). Treatment of 19 with liquid ammonia effected not only a smooth removal of the blocking groups, but also an aminolysis of the ester function which was then followed by a ring annulation to provide 8-aza-3-deazaguanosine (2). The structures of these nucleosides were established on the basis of proton nmr spectral data and nuclear Overhauser enhancement data. The nucleosides obtained in this study were also converted through a chemical degradation sequence into nucleosides which had been obtained during an earlier work from our laboratory. The present study also provides unequivocal proof of the structures of some triazole nucleosides obtained in the earlier study.

500-MHz proton NMR study of poly(dG).cntdot.poly(dC) in solution using one-dimensional nuclear Overhauser effect

Biochemistry ◽  
1986 ◽  
Vol 25 (12) ◽  
pp. 3659-3665 ◽  
Author(s):  
Mukti H. Sarma ◽  
Goutam Gupta ◽  
Ramaswamy H. Sarma
Keyword(s):  
Nuclear Overhauser Effect ◽  
Proton Nmr ◽  
One Dimensional ◽  
Overhauser Effect ◽  
Nmr Study ◽  
Nuclear Overhauser

Sequential proton NMR assignments and secondary structure of the B domain of staphylococcal protein A: structural changes between the free B domain in solution and the Fc-bound B domain in crystal

Biochemistry ◽  
1990 ◽  
Vol 29 (37) ◽  
pp. 8787-8793 ◽  
Author(s):  
Hidetaka Torigoe ◽  
Ichio Shimada ◽  
Akiko Saito ◽  
Moriyuki Sato ◽  
Yoji Arata
Keyword(s):  
Secondary Structure ◽  
Structural Changes ◽  
Nmr Assignments ◽  
Protein A ◽  
Proton Nmr ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein

A two-dimensional nuclear overhauser enhancement experiment using semiselective soft pulses, and its applications to proteins

1988 ◽  
Vol 78 (2) ◽  
pp. 371-375 ◽  
Author(s):  
Hartmut Oschkinat ◽  
G Marius Clore ◽  
Angela M Gronenborn
Keyword(s):  
Two Dimensional ◽  
Nuclear Overhauser Enhancement ◽  
Nuclear Overhauser
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