Nuclear magnetic resonance study of the complexes of manganese(II) and fully adenylylated glutamine synthetase (Escherichia coli W). Frequency, temperature, and substrate dependence of water proton relaxation rates

Biochemistry ◽  
1974 ◽  
Vol 13 (16) ◽  
pp. 3286-3291 ◽  
Author(s):  
Joseph J. Villafranca ◽  
Fred C. Wedler
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic Resonance Study ◽  
Water Proton ◽  
Proton Relaxation ◽  
Relaxation Rates ◽  
Magnetic Resonance Study ◽  
Substrate Dependence ◽  
Frequency Temperature

A Carbon-13 Nuclear Magnetic Resonance Study of Binding of Copper(II) to Pyrimidine Nucleotides and Nucleosides

1974 ◽  
Vol 52 (6) ◽  
pp. 924-929 ◽  
Author(s):  
George Kotowycz
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic Resonance Study ◽  
Relaxation Rates ◽  
Magnetic Resonance Study ◽  
Pyrimidine Nucleotides ◽  
Pyrimidine Nucleosides ◽  
Nucleotides And Nucleosides ◽  
Longitudinal Relaxation ◽  
Nuclear Magnetic

The influence of paramagnetic Cu2+ ions on the proton decoupled 13C n.m.r. spectra of pyrimidine nucleosides and nucleotides has been studied. For 5′-CMP, the C5 resonance is broadened first on the addition of Cu2+ ions followed by the C2, C4, and C1′ resonances. From a comparison of the transverse and longitudinal relaxation rates of the base carbon nuclei due to the presence of Cu2+ ions, binding of Cu2+ to the N3 nitrogen of 5′-CMP is predicted. A similar broadening behavior is observed for 5′-UMP, 5′-TMP, cytidine, and uridine. This indicates that the Cu2+ ion is located near the N3 nitrogen in these Cu2+-nucleoside and -nucleotide complexes.

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