Composition and structure of plasma lipoproteins. Separation and quantification of the lipoprotein families occurring in the high density lipoproteins of human plasma

Biochemistry ◽  
1972 ◽  
Vol 11 (18) ◽  
pp. 3419-3428 ◽  
Author(s):  
G. Kostner ◽  
P. Alaupovic
Keyword(s):  
Human Plasma ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Composition And Structure

scholarly journals IMMUNOCHEMICAL STUDIES ON HUMAN PLASMA LIPOPROTEINS

1957 ◽  
Vol 105 (1) ◽  
pp. 49-67 ◽  
Author(s):  
Frederick Aladjem ◽  
Miriam Lieberman ◽  
John W. Gofman
Keyword(s):  
Human Plasma ◽  
Plasma Protein ◽  
Protein Fraction ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Plasma Protein Fraction ◽  
Free Plasma

Low density human plasma lipoproteins Sf 17+, Sf 13, and Sf 6, high density lipoproteins 2 and 3, and a lipoprotein-free plasma protein fraction were isolated from human plasma by ultracentrifugal methods. It was found that human plasma lipoproteins are immunochemically distinct from the non-lipoprotein containing plasma protein fraction. Lipoprotein fractions of a given hydrated density, isolated from different individuals, were found to be immunochemically indistinguishable by qualitative absorption tests. Qualitative antigenic differences were shown to exist between low density lipoproteins and high density lipoproteins. Quantitative precipitin reactions showed that low density lipoproteins Sf 6 and Sf 13 were immunochemically very similar. However, they differed with respect to the amount of antigen nitrogen required for maximum precipitation. Agar diffusion analyses were performed; the results suggest heterogeneity of lipoproteins by this criterion.

Thromboplastic Effects ot Human Plasma Lipoproteins

1975 ◽  
Author(s):  
L.-O. Andersson ◽  
H. Sandberg
Keyword(s):  
Human Plasma ◽  
Platelet Rich Plasma ◽  
Density Lipoprotein ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Phospholipid Content ◽  
Factor X ◽  
Promoting Effect ◽  
Free Plasma

Lipoprotein fractions from human plasma was prepared by ultracentrifugal flotation. Additions of those fractions to plasma containing various amounts of platelets showed that in platelet-poor and platelet-free plasma there was a clear clot-promoting effect of the additions. In platelet-rich plasma, this effect was negligible. Measurements on the thrombo-plastine and Stypven clotting times showed that the high density lipoprotein fraction affected both the prothrombin and the Factor X activation steps whereas the low density lipoproteins only influenced the prothrombin activation step. Addition of antibodies against high density lipoproteins to platelet-free plasma caused a prolongation of the thromboplastin time.The relation between lipoprotein structure, phospholipid content and thromboplastic effects is dicussed.

scholarly journals Characterization of plasma lipoproteins separated and purified by agarose-column chromatography

1974 ◽  
Vol 139 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Lawrence L. Rudel ◽  
Jason A. Lee ◽  
Manford D. Morris ◽  
James M. Felts
Keyword(s):  
Human Plasma ◽  
Column Chromatography ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Simple Method ◽  
Lipoprotein Class

1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.

Factors affecting the conversion of high-density lipoproteins: experiments with pig and human plasma

1985 ◽  
Vol 835 (2) ◽  
pp. 244-252 ◽  
Author(s):  
Gabriele Knipping ◽  
Rudolf Zechner ◽  
Gerhard Max Kostner ◽  
Anton Holasek
Keyword(s):  
Human Plasma ◽  
High Density ◽  
High Density Lipoproteins ◽  
Factors Affecting

scholarly journals A 70-kDa apolipoprotein designated ApoJ is a marker for subclasses of human plasma high density lipoproteins.

1990 ◽  
Vol 265 (22) ◽  
pp. 13240-13247 ◽  
Author(s):  
H V de Silva ◽  
W D Stuart ◽  
C R Duvic ◽  
J R Wetterau ◽  
M J Ray ◽  
...  
Keyword(s):  
Human Plasma ◽  
High Density ◽  
High Density Lipoproteins ◽  
Plasma High Density
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