Phospholipid-requiring bacterial enzyme. II. Role of phospholipid in the uridine diphosphate galactose: lipopolysaccharide α-3-galactosyl transferase reaction

Biochemistry ◽  
1969 ◽  
Vol 8 (9) ◽  
pp. 3508-3515 ◽  
Author(s):  
Akira Endo ◽  
Lawrence Rothfield
Keyword(s):  
Uridine Diphosphate ◽  
Galactosyl Transferase ◽  
Bacterial Enzyme ◽  
Uridine Diphosphate Galactose

scholarly journals Kinetic studies on the effect of uridine diphosphate galactose and manganous ions on the reaction between lactose synthetase A protein from human milk and p-hydroxymercuribenzoate

1974 ◽  
Vol 143 (3) ◽  
pp. 587-590 ◽  
Author(s):  
Barry J. Kitchen ◽  
Patrick Andrews
Keyword(s):  
Human Milk ◽  
Protective Effect ◽  
Rate Constants ◽  
Dissociation Constants ◽  
Kinetic Studies ◽  
Uridine Diphosphate ◽  
First Order ◽  
Pseudo First Order ◽  
Uridine Diphosphate Galactose

The inhibition of lactose synthetase A protein by p-hydroxymercuribenzoate at pH7.5 and 25°C, which involves the reaction of one molecule of inhibitor with each molecule of enzyme, was decreased in rate by UDP-galactose, especially in the presence of Mn2+. Pseudo-first-order rate constants for the reaction between 0.1mm-p-hydroxymercuribenzoate and free enzyme, the enzyme–UDP-galactose complex and the enzyme–Mn2+–UDP-galactose complex were 4.4×10−2, 1.9×10−2 and 0.3×10−2min−1 respectively. The results also indicated that dissociation constants for UDP-galactose in the enzyme–UDP-galactose and enzyme–Mn2+–UDP-galactose complexes were 313 and 16μm respectively, the latter value being similar to the Km for UDP-galactose in the lactose synthetase reaction. The protective effect of UDP-galactose and the role of Mn2+ ions in lactose synthetase are discussed.

scholarly journals Enzymatic deficiency in neurological mutants. Brain uridine diphosphate galactose: Ceramide galactosyl transferase in jimpy mouse

FEBS Letters ◽  
1970 ◽  
Vol 8 (4) ◽  
pp. 213-216 ◽  
Author(s):  
N.M. Neskovic ◽  
J.L. Nussbaum ◽  
P. Mandel
Keyword(s):  
Uridine Diphosphate ◽  
Galactosyl Transferase ◽  
Jimpy Mouse ◽  
Uridine Diphosphate Galactose

scholarly journals Studies on the Mechanism of Action of Uridine Diphosphate-galactose-4-epimerase

1972 ◽  
Vol 247 (4) ◽  
pp. 1339-1342
Author(s):  
Timple G. Wee ◽  
Janet Davis ◽  
Perry A. Frey
Keyword(s):  
Mechanism Of Action ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Galactose

A case of uridine diphosphate galactose‐4‐epimerase deficiency detected by neonatal screening for galactosaemia

1986 ◽  
Vol 144 (3) ◽  
pp. 150-151 ◽  
Author(s):  
Francis G. Bowling ◽  
David K.B. Fraser ◽  
Alan E. Clague ◽  
Darryl J. Morris ◽  
Alan Hayes
Keyword(s):  
Neonatal Screening ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Galactose

scholarly journals Purification of Uridine Diphosphate Galactose-4-epimerase from Yeast, and the Identification of Protein-bound Diphosphopyridine Nucleotide

1960 ◽  
Vol 235 (2) ◽  
pp. 308-312 ◽  
Author(s):  
Elizabeth S. Maxwell ◽  
Huguette de Robichon-Szulmajster
Keyword(s):  
Uridine Diphosphate ◽  
Uridine Diphosphate Galactose
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