Characterization of the Membrane Binding Mode of the C2 Domain of PKCε†

Biochemistry ◽  
2003 ◽  
Vol 42 (40) ◽  
pp. 11661-11668 ◽  
Author(s):  
Senena Corbalán-Garcia ◽  
Susana Sánchez-Carrillo ◽  
Josefa García-García ◽  
Juan C. Gómez-Fernández
Keyword(s):  
Membrane Binding ◽  
Binding Mode ◽  
C2 Domain

scholarly journals Characterization of α-crystallin-plasma membrane binding.

2002 ◽  
Vol 277 (2) ◽  
pp. 1628
Author(s):  
Brian A. Cobb ◽  
J. Mark Petrash
Keyword(s):  
Plasma Membrane ◽  
Membrane Binding ◽  
Plasma Membrane Binding

scholarly journals Characterization of Multiple C2 Domain and Transmembrane Region Proteins in Arabidopsis

2017 ◽  
Vol 176 (3) ◽  
pp. 2119-2132 ◽  
Author(s):  
Lu Liu ◽  
Chunying Li ◽  
Zhe Liang ◽  
Hao Yu
Keyword(s):  
C2 Domain ◽  
Transmembrane Region

scholarly journals The C2A-C2B Linker Defines the High Affinity Ca2+ Binding Mode of Rabphilin-3A

2006 ◽  
Vol 282 (7) ◽  
pp. 5015-5025 ◽  
Author(s):  
Pierre Montaville ◽  
Christine Schlicker ◽  
Andrei Leonov ◽  
Markus Zweckstetter ◽  
George M. Sheldrick ◽  
...  
Keyword(s):  
Binding Mode ◽  
Bound State ◽  
C2 Domain ◽  
Binding Assays ◽  
Host Proteins ◽  
Binding Properties ◽  
C2 Domains ◽  
Phospholipid Binding ◽  
Acidic Residues ◽  
Structural Base

The Ca2+ binding properties of C2 domains are essential for the function of their host proteins. We present here the first crystal structures showing an unexpected Ca2+ binding mode of the C2B domain of rabphilin-3A in atomic detail. Acidic residues from the linker region between the C2A and C2B domains of rabphilin-3A interact with the Ca2+-binding region of the C2B domain. Because of these interactions, the coordination sphere of the two bound Ca2+ ions is almost complete. Mutation of these acidic residues to alanine resulted in a 10-fold decrease in the intrinsic Ca2+ binding affinity of the C2B domain. Using NMR spectroscopy, we show that this interaction occurred only in the Ca2+-bound state of the C2B domain. In addition, this Ca2+ binding mode was maintained in the C2 domain tandem fragment. In NMR-based liposome binding assays, the linker was not released upon phospholipid binding. Therefore, this unprecedented Ca2+ binding mode not only shows how a C2 domain increases its intrinsic Ca2+ affinity, but also provides the structural base for an atypical protein-Ca2+-phospholipid binding mode of rabphilin-3A.

Cloning, expression and functional characterization of the C2 domain from tomato phospholipase Dα

2011 ◽  
Vol 49 (1) ◽  
pp. 18-32 ◽  
Author(s):  
Krishnaraj Tiwari ◽  
Gopinadhan Paliyath
Keyword(s):  
Functional Characterization ◽  
C2 Domain ◽  
Phospholipase Dα

Isolation and Characterization of Membrane Binding Proteins

1976 ◽  
pp. 183-242 ◽  
Author(s):  
Dale L. Oxender ◽  
Steven C. Quay
Keyword(s):  
Binding Proteins ◽  
Membrane Binding ◽  
Isolation And Characterization

scholarly journals Characterization of the binding mode of JNK-interacting protein 1 (JIP1) to kinesin-light chain 1 (KLC1)

2018 ◽  
Vol 293 (36) ◽  
pp. 13946-13960 ◽  
Author(s):  
T. Quyen Nguyen ◽  
Magali Aumont-Nicaise ◽  
Jessica Andreani ◽  
Christophe Velours ◽  
Mélanie Chenon ◽  
...  
Keyword(s):  
Light Chain ◽  
Binding Mode ◽  
Interacting Protein ◽  
Kinesin Light Chain

Characterization of new PPARγ agonists: Benzimidazole derivatives—importance of positions 5 and 6, and computational studies on the binding mode

2010 ◽  
Vol 18 (16) ◽  
pp. 5885-5895 ◽  
Author(s):  
Matthias Goebel ◽  
Gerhard Wolber ◽  
Patrick Markt ◽  
Bart Staels ◽  
Thomas Unger ◽  
...  
Keyword(s):  
Binding Mode ◽  
Benzimidazole Derivatives ◽  
Computational Studies ◽  
Pparγ Agonists

scholarly journals Membrane-binding properties of the Factor VIII C2 domain

2011 ◽  
Vol 435 (1) ◽  
pp. 187-196 ◽  
Author(s):  
Valerie A. Novakovic ◽  
David B. Cullinan ◽  
Hironao Wakabayashi ◽  
Philip J. Fay ◽  
James D. Baleja ◽  
...  
Keyword(s):  
Factor Viii ◽  
Structural Integrity ◽  
Membrane Binding ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Binding Motif ◽  
C2 Domain ◽  
Factor X ◽  
Factor Ixa ◽  
Factor Viiia

Factor VIII functions as a cofactor for Factor IXa in a membrane-bound enzyme complex. Membrane binding accelerates the activity of the Factor VIIIa–Factor IXa complex approx. 100000-fold, and the major phospholipid-binding motif of Factor VIII is thought to be on the C2 domain. In the present study, we prepared an fVIII-C2 (Factor VIII C2 domain) construct from Escherichia coli, and confirmed its structural integrity through binding of three distinct monoclonal antibodies. Solution-phase assays, performed with flow cytometry and FRET (fluorescence resonance energy transfer), revealed that fVIII-C2 membrane affinity was approx. 40-fold lower than intact Factor VIII. In contrast with the similarly structured C2 domain of lactadherin, fVIII-C2 membrane binding was inhibited by physiological NaCl. fVIII-C2 binding was also not specific for phosphatidylserine over other negatively charged phospholipids, whereas a Factor VIII construct lacking the C2 domain retained phosphatidyl-L-serine specificity. fVIII-C2 slightly enhanced the cleavage of Factor X by Factor IXa, but did not compete with Factor VIII for membrane-binding sites or inhibit the Factor Xase complex. Our results indicate that the C2 domain in isolation does not recapitulate the characteristic membrane binding of Factor VIII, emphasizing that its role is co-operative with other domains of the intact Factor VIII molecule.

Characterization of neurocalcin delta membrane binding by biophysical methods

2019 ◽  
Vol 174 ◽  
pp. 291-299 ◽  
Author(s):  
Emmanuelle Hoareau ◽  
Nicolas Belley ◽  
Kristina Klinker ◽  
Bernard Desbat ◽  
Élodie Boisselier
Keyword(s):  
Membrane Binding

scholarly journals Regulation of Membrane Binding by the C2-Domain of Cytoplasmic Phospholipase A2 by Ceramide-1-Phosphate and Calcium

2017 ◽  
Vol 112 (3) ◽  
pp. 392a-393a
Author(s):  
Xiuhong Zhai ◽  
Yong-Guang Gao ◽  
Ivan A. Boldyrev ◽  
Lucy Malinina ◽  
Dinshaw J. Patel ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Membrane Binding ◽  
C2 Domain ◽  
Cytoplasmic Phospholipase A2 ◽  
Ceramide 1
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