The Role of Electrostatics in the Interaction of the Inhibitory Region of Troponin I with Troponin C†

Biochemistry ◽  
2005 ◽  
Vol 44 (45) ◽  
pp. 14750-14759 ◽  
Author(s):  
Darrin A. Lindhout ◽  
Robert F. Boyko ◽  
David C. Corson ◽  
Monica X. Li ◽  
Brian D. Sykes
Keyword(s):  
Troponin I ◽  
Troponin C ◽  
Inhibitory Region

scholarly journals The role of the NH2- and COOH-terminal domains of the inhibitory region of troponin I in the regulation of skeletal muscle contraction.

2000 ◽  
Vol 275 (10) ◽  
pp. 7438
Author(s):  
Danuta Szczesna ◽  
Ren Zhang ◽  
Jiaju Zhao ◽  
Michelle Jones ◽  
James D. Potter
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Troponin I ◽  
Skeletal Muscle Contraction ◽  
Inhibitory Region ◽  
Terminal Domains

scholarly journals Cross-linking of residue 57 in the regulatory domain of a mutant rabbit skeletal muscle troponin C to the inhibitory region of troponin I

1991 ◽  
Vol 266 (21) ◽  
pp. 13746-13751
Author(s):  
T. Kobayashi ◽  
T. Tao ◽  
Z. Grabarek ◽  
J. Gergely ◽  
J.H. Collins
Keyword(s):  
Skeletal Muscle ◽  
Troponin I ◽  
Troponin C ◽  
Rabbit Skeletal Muscle ◽  
Cross Linking ◽  
Regulatory Domain ◽  
Inhibitory Region

Calmodulin and troponin C: affinity chromatographic study of divalent cation requirements for troponin I inhibitory peptide (residues 104–115), mastoparan, and fluphenazine binding

1987 ◽  
Vol 65 (11) ◽  
pp. 982-988 ◽  
Author(s):  
Jennifer E. Van Eyk ◽  
Robert S. Hodges
Keyword(s):  
Troponin I ◽  
Troponin C ◽  
Homologous Region ◽  
Affinity Column ◽  
Chromatographic Study ◽  
Inhibitory Peptide ◽  
Receptor Sites ◽  
Inhibitory Region ◽  
Correct Alignment ◽  
Correct Structure

The different conformations induced by the binding of Mg2+ or Ca2+ to troponin C (TnC) and calmodulin (CaM) results in the exposure of various interfaces with potential to bind target compounds. The interaction of TnC or CaM with three affinity columns with ligands of either the synthetic peptide of troponin I (TnI) inhibitory region (residues 104–115), mastoparan (a wasp venom peptide), or fluphenazine (a phenothiazine drug) were investigated in the presence of Mg2+ or Ca2+. TnC and CaM in the presence of either Ca2+ or Mg2+ bound to the TnI peptide 104–115. The cation specificity for this interaction firmly establishes that the TnI inhibitory region binds to the high affinity sites of TnC (most likely the N-terminal helix of site III) and presumably the homologous region of CaM. Mastoparan interacted strongly with both proteins in the presence of Ca2+ but, in the presence of Mg2+, did not bind to TnC and only bound weakly to CaM. Fluphenazine bound to TnC and CaM only in the presence of Ca2+. When the ligands interacted with either proteins there was an increase in cation affinity, such that TnC and CaM were eluted from the TnI peptide or mastoparan affinity column with 0.1 M EDTA compared with the 0.01 M EDTA required to elute the proteins from the fluphenazine column. The interaction of these ligands with their receptor sites on TnC and CaM require a specific and spatially correct alignment of hydrophobic and negatively charged residues on these proteins. In the case of the TnI peptide, which represents a naturally occurring target protein for TnC, Mg2+ and Ca2+ can induce the correct structure in TnC or CaM for interaction, while only Ca2+ can induce the correct structure for mastoparan or fluophenazine binding.

Role of the Structural Domain of Troponin C in Muscle Regulation:  NMR Studies of Ca2+Binding and Subsequent Interactions with Regions 1−40 and 96−115 of Troponin I†

Biochemistry ◽  
2000 ◽  
Vol 39 (11) ◽  
pp. 2902-2911 ◽  
Author(s):  
Pascal Mercier ◽  
Monica X. Li ◽  
Brian D. Sykes
Keyword(s):  
Troponin I ◽  
Troponin C ◽  
Structural Domain ◽  
Nmr Studies ◽  
Muscle Regulation

scholarly journals A model of troponin-I in complex with troponin-C using hybrid experimental data: The inhibitory region is a β-hairpin

2000 ◽  
Vol 9 (7) ◽  
pp. 1312-1326 ◽  
Author(s):  
Michael E. Wall ◽  
Stephen C. Gallagher ◽  
Jill Trewhella ◽  
Chang-Shung Tung
Keyword(s):  
Experimental Data ◽  
Troponin I ◽  
Troponin C ◽  
Inhibitory Region
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