Dissociation Kinetics of the GroEL−gp31 Chaperonin Complex Studied with Förster Resonance Energy Transfer

Biochemistry ◽  
2009 ◽  
Vol 48 (49) ◽  
pp. 11692-11698 ◽  
Author(s):  
Stéphane Calmat ◽  
Johnny Hendriks ◽  
Harm van Heerikhuizen ◽  
Christoph F. Schmidt ◽  
Saskia M. van der Vies ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dissociation Kinetics ◽  
Kinetics Of

Kinetics of the Structural Transition of Muscle Thin Filaments Observed by Fluorescence Resonance Energy Transfer†

Biochemistry ◽  
2004 ◽  
Vol 43 (33) ◽  
pp. 10739-10747 ◽  
Author(s):  
Yuji Shitaka ◽  
Chieko Kimura ◽  
Takayoshi Iio ◽  
Masao Miki
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Structural Transition ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Thin Filaments ◽  
Fluorescence Resonance ◽  
Kinetics Of

Nonextensive kinetics of fluorescence resonance energy transfer

2008 ◽  
Vol 129 (14) ◽  
pp. 144507 ◽  
Author(s):  
Olaf J. Rolinski ◽  
David J. S. Birch
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Resonance ◽  
Kinetics Of

Direct measurement of the kinetics of CBM9 fusion-tag bioprocessing using luminescence resonance energy transfer

2009 ◽  
Vol 25 (3) ◽  
pp. 874-881 ◽  
Author(s):  
Mojgan Kavoosi ◽  
A. Louise Creagh ◽  
Robin F. B. Turner ◽  
Douglas G. Kilburn ◽  
Charles A. Haynes
Keyword(s):  
Energy Transfer ◽  
Direct Measurement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fusion Tag ◽  
Kinetics Of

Monitoring the intracellular transformation process of surface-cleavable PLGA particles containing disulfide bonds by fluorescence resonance energy transfer

2015 ◽  
Vol 3 (45) ◽  
pp. 8865-8873 ◽  
Author(s):  
Dahai Yu ◽  
Guangyang Zou ◽  
Xiaojing Cui ◽  
Zhengwei Mao ◽  
Irina Estrela-Lopis ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Disulfide Bonds ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Transformation Process ◽  
Fluorescence Resonance ◽  
Kinetics Of ◽  
Surface Cleavage ◽  
In Cells

The FRET technique was used to quantify the surface cleavage kinetics of PLGA particles containing disulfide bonds in cells.

Calmodulin conformational changes in the activation of protein kinases

2002 ◽  
Vol 30 (2) ◽  
pp. 55-61 ◽  
Author(s):  
K. Török
Keyword(s):  
Energy Transfer ◽  
Protein Kinases ◽  
Conformational Changes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Activation Process ◽  
Dependent Protein ◽  
Peptide Complexes ◽  
Muscle Myosin ◽  
Kinetics Of

The conformation of Ca2+/calmodulin changes from extended when free in solution to compact when bound in peptide complexes. The extent and kinetics of calmodulin compaction in association with Ca2+/calmodulin-dependent protein kinases (CaMKs), as well as target peptides, were investigated by fluorescence, resonance energy transfer and stopped-flow kinetics. Compaction of Ca2+/ calmodulin labelled with resonance energy-transfer probes in association with target peptides was rapid (>350 s−1). With the target enzymes smooth-muscle myosin light-chain kinase, CaMKIV and αcCaMKII, the rates of calmodulin compaction were one-two orders of magnitude lower compared with those of the peptides and in the case of αCaMKII, ATP binding and Thr286 auto-phosphorylation were required for calmodulin compaction. In the absence of nucleotides, Ca2+/calmodulin bound to αCaMKII in extended conformations, initially probably attached by one lobe only. Kinetic data suggest that in the activation process of Ca2+/ calmodulin-dependent protein kinases, productive as well as unproductive complexes are formed. The formation of productive complexes with Ca2+/calmodulin thus may determine the rate of activation.

scholarly journals Förster Resonance Energy Transfer beyond 10 nm: Exploiting the Triplet State Kinetics of Organic Fluorophores

2011 ◽  
Vol 115 (45) ◽  
pp. 13360-13370 ◽  
Author(s):  
Heike Hevekerl ◽  
Thiemo Spielmann ◽  
Andriy Chmyrov ◽  
Jerker Widengren
Keyword(s):  
Energy Transfer ◽  
Triplet State ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Organic Fluorophores ◽  
Kinetics Of ◽  
Förster Resonance

scholarly journals Real time kinetics of restriction endonuclease cleavage monitored by fluorescence resonance energy transfer

1994 ◽  
Vol 22 (15) ◽  
pp. 3155-3159 ◽  
Author(s):  
Soumitra S. Ghosh ◽  
Peggy S. Eis ◽  
Kirsten Blumeyer ◽  
Kim Fearon ◽  
David P. Millar
Keyword(s):  
Energy Transfer ◽  
Restriction Endonuclease ◽  
Real Time ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Resonance ◽  
Kinetics Of ◽  
Restriction Endonuclease Cleavage
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