Crystal Structure and Photodynamic Behavior of the Blue Emission Variant Y66H/Y145F of Green Fluorescent Protein†

Biochemistry ◽  
1997 ◽  
Vol 36 (32) ◽  
pp. 9759-9765 ◽  
Author(s):  
Rebekka M. Wachter ◽  
Brett A. King ◽  
Roger Heim ◽  
Karen Kallio ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Blue Emission ◽  
Green Fluorescent

scholarly journals The structure of a GFP-based antibody (fluorobody) to TLH, a toxin fromVibrio parahaemolyticus

2015 ◽  
Vol 71 (7) ◽  
pp. 913-918 ◽  
Author(s):  
Yaoguang Chen ◽  
Xiaocheng Huang ◽  
Rongzhi Wang ◽  
Shihua Wang ◽  
Ning Shi
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent Protein ◽  
Hybrid Molecule ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Food Borne ◽  
Vapour Diffusion ◽  
Green Fluorescent

A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteriumVibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space groupP43212, with unit-cell parametersa=b= 63.35,c = 125.90 Å, were obtained by vapour diffusion in hanging drops and the structure was refined to anRfreeof 16.7% at 1.5 Å resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.

scholarly journals A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47331 ◽  
Author(s):  
Jion M. Battad ◽  
Daouda A. K. Traore ◽  
Emma Byres ◽  
Jamie Rossjohn ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
X Ray ◽  
Green Fluorescent

Crystal Structure of the Aequorea victoria Green Fluorescent Protein

Science ◽  
1996 ◽  
Vol 273 (5280) ◽  
pp. 1392-1395 ◽  
Author(s):  
M. Orm  ◽  
A. B. Cubitt ◽  
K. Kallio ◽  
L. A. Gross ◽  
R. Y. Tsien ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Aequorea Victoria ◽  
Green Fluorescent

Effects of the benzoxazole group on green fluorescent protein chromophore crystal structure and solid state photophysics

2016 ◽  
Vol 4 (14) ◽  
pp. 2793-2801 ◽  
Author(s):  
Abdelhamid Ghodbane ◽  
W. Brett Fellows ◽  
John R. Bright ◽  
Debashree Ghosh ◽  
Nathalie Saffon ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Solid State ◽  
Fluorescent Protein ◽  
Aggregation Induced Emission ◽  
Emission Enhancement ◽  
Green Fluorescent ◽  
Unusual Type

Most of the conjugates exhibited aggregation-induced emission enhancement, probably involving a unusual type of excimer.

The 1.7 Å Crystal Structure of Dronpa: A Photoswitchable Green Fluorescent Protein

2006 ◽  
Vol 364 (2) ◽  
pp. 213-224 ◽  
Author(s):  
Pascal G. Wilmann ◽  
Kristina Turcic ◽  
Jion M. Battad ◽  
Matthew C.J. Wilce ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

Crystal Structure of the pH-Dependent Green Fluorescent Protein WasCFP with a Tryptophan-Based Chromophore at an Extremely Low pH of 2.0

2018 ◽  
Vol 44 (6) ◽  
pp. 640-644
Author(s):  
N. V. Pletneva ◽  
R. G. Efremov ◽  
E. A. Goryacheva ◽  
I. V. Artemyev ◽  
S. F. Arkhipova ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Low Ph ◽  
Ph Dependent ◽  
Green Fluorescent

scholarly journals Split green fluorescent protein as a modular binding partner for protein crystallization

2013 ◽  
Vol 69 (12) ◽  
pp. 2513-2523 ◽  
Author(s):  
Hau B. Nguyen ◽  
Li-Wei Hung ◽  
Todd O. Yeates ◽  
Thomas C. Terwilliger ◽  
Geoffrey S. Waldo
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Protein Crystallization ◽  
Target Protein ◽  
Surface Loop ◽  
Binding Partner ◽  
Green Fluorescent ◽  
The Way

A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10–11) hairpin in complex with GFP(1–9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10–11) hairpin with a variety of GFP(1–9) mutants engineered for favorable crystallization.

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