Effects of the benzoxazole group on green fluorescent protein chromophore crystal structure and solid state photophysics

2016 ◽  
Vol 4 (14) ◽  
pp. 2793-2801 ◽  
Author(s):  
Abdelhamid Ghodbane ◽  
W. Brett Fellows ◽  
John R. Bright ◽  
Debashree Ghosh ◽  
Nathalie Saffon ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Solid State ◽  
Fluorescent Protein ◽  
Aggregation Induced Emission ◽  
Emission Enhancement ◽  
Green Fluorescent ◽  
Unusual Type

Most of the conjugates exhibited aggregation-induced emission enhancement, probably involving a unusual type of excimer.

scholarly journals The structure of a GFP-based antibody (fluorobody) to TLH, a toxin fromVibrio parahaemolyticus

2015 ◽  
Vol 71 (7) ◽  
pp. 913-918 ◽  
Author(s):  
Yaoguang Chen ◽  
Xiaocheng Huang ◽  
Rongzhi Wang ◽  
Shihua Wang ◽  
Ning Shi
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent Protein ◽  
Hybrid Molecule ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Food Borne ◽  
Vapour Diffusion ◽  
Green Fluorescent

A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteriumVibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space groupP43212, with unit-cell parametersa=b= 63.35,c = 125.90 Å, were obtained by vapour diffusion in hanging drops and the structure was refined to anRfreeof 16.7% at 1.5 Å resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.

Locked ortho- and para-Core Chromophores of Green Fluorescent Protein; Dramatic Emission Enhancement via Structural Constraint

2014 ◽  
Vol 136 (33) ◽  
pp. 11805-11812 ◽  
Author(s):  
Yen-Hao Hsu ◽  
Yi-An Chen ◽  
Huan-Wei Tseng ◽  
Zhiyun Zhang ◽  
Jiun-Yi Shen ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Structural Constraint ◽  
Emission Enhancement ◽  
Green Fluorescent

Synthesis and properties of geometrical 4-diarylmethylene analogs of the green fluorescent protein chromophore

2018 ◽  
Vol 16 (14) ◽  
pp. 2397-2401 ◽  
Author(s):  
Masahiro Ikejiri ◽  
Haruka Kojima ◽  
Yuumi Fugono ◽  
Aki Fujisaka ◽  
Yoshiko Chihara ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Aggregation Induced Emission ◽  
Gfp Chromophore ◽  
Green Fluorescent

The E- and Z-isomers of E/Z-photoisomerizable diarylmethylene analogs of the GFP chromophore (geo-DAIN) produce different colors of aggregation-induced emission (AIE).

scholarly journals A Green Fluorescent Protein Containing a QFG Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47331 ◽  
Author(s):  
Jion M. Battad ◽  
Daouda A. K. Traore ◽  
Emma Byres ◽  
Jamie Rossjohn ◽  
Rodney J. Devenish ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Optical Properties ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
X Ray ◽  
Green Fluorescent

Crystal Structure and Photodynamic Behavior of the Blue Emission Variant Y66H/Y145F of Green Fluorescent Protein†

Biochemistry ◽  
1997 ◽  
Vol 36 (32) ◽  
pp. 9759-9765 ◽  
Author(s):  
Rebekka M. Wachter ◽  
Brett A. King ◽  
Roger Heim ◽  
Karen Kallio ◽  
Roger Y. Tsien ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Blue Emission ◽  
Green Fluorescent

Environmentally friendly luminescent solar concentrators based on an optically efficient and stable green fluorescent protein

2020 ◽  
Vol 22 (15) ◽  
pp. 4943-4951 ◽  
Author(s):  
Carlota P. A. Carlos ◽  
Sandra F. H. Correia ◽  
Margarida Martins ◽  
Oleksandr A. Savchuk ◽  
João A. P. Coutinho ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Solid State ◽  
Power Conversion Efficiency ◽  
Conversion Efficiency ◽  
Fluorescent Protein ◽  
Power Conversion ◽  
Environmentally Friendly ◽  
Solar Concentrators ◽  
Luminescent Solar Concentrators ◽  
Green Fluorescent

Green fluorescent protein was used to fabricate planar LSCs in liquid and solid state, yielding competitive power conversion efficiency values stating the potential of naturally-based molecules in the development of sustainable LSCs.

Sign in / Sign up

Export Citation Format

Share Document