Influence of Net Protein Charge and Stationary Phase Charge on Protein Retention in Size Exclusion Chromatography

1996 ◽  
pp. 88-102 ◽  
Author(s):  
Vincent A. Romano ◽  
Tony Ebeyer ◽  
Paul L. Dubin
Keyword(s):  
Stationary Phase ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Protein Charge ◽  
Protein Retention ◽  
Exclusion Chromatography ◽  
Net Protein

scholarly journals Molar mass characterization of hydrophilic copolymers, 2 Size exclusion chromatography of cationic (meth)acrylate copolymers

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Martina Adler ◽  
Harald Pasch ◽  
Christian Meier ◽  
Raimund Senger ◽  
Hans-Günter Koban ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Size Exclusion Chromatography ◽  
Molar Mass ◽  
Extended Period ◽  
Size Exclusion ◽  
Viscosity Data ◽  
Exclusion Chromatography ◽  
Reproducible Method ◽  
Novel Method ◽  
Sec System

AbstractA robust and reproducible method for the molar mass analysis of cationic copolymers based on dimethylaminoethyl methacrylate or trimethylammonioethyl methacrylate and different (meth)acrylates has been developed. Size exclusion chromatography (SEC) using a novel polyester-based packing as the stationary phase and dimethylacetamide (DMAC) as the mobile phase yields highly accurate results for copolymers with an amino comonomer content up to 50 wt.-%. To suppress the different polar and ionic interactions between sample molecules, stationary phase and eluent, DMAC was modified with LiBr and tris(hydroxymethylamino) methane (TRIS). Calibrating the SEC system with poly(methyl methacrylate) of narrow polydispersity, molar masses were obtained that are in good agreement with viscosity data. Reproducibility and robustness of the novel method were proven by running samples for an extended period of two weeks.

Surface structure of size exclusion chromatography stationary phase

2007 ◽  
Vol 227 (2) ◽  
pp. 110-117 ◽  
Author(s):  
A. KALE ◽  
M. CHERYAN ◽  
S. ROBINSON ◽  
S. MACLAREN
Keyword(s):  
Stationary Phase ◽  
Surface Structure ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography

Inverse Size Exclusion Chromatography Using Extraction Residues or Extracts of Coals as a Stationary Phase

1996 ◽  
Vol 10 (4) ◽  
pp. 1012-1016 ◽  
Author(s):  
Mitsuru Morino ◽  
Hiroyuki Kaneko ◽  
Toshimasa Takanohashi ◽  
Masashi Iino
Keyword(s):  
Stationary Phase ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Inverse Size Exclusion Chromatography

Molar mass characterization of hydrophilic copolymers, 1 Size exclusion chromatography of neutral and anionic (meth)acrylate copolymers

e-Polymers ◽  
2004 ◽  
Vol 4 (1) ◽  
Author(s):  
Martina Adler ◽  
Harald Pasch ◽  
Christian Meier ◽  
Raimund Senger ◽  
Hans-Günter Koban ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Size Exclusion Chromatography ◽  
Methacrylic Acid ◽  
Molar Mass ◽  
Extended Period ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Reproducible Method ◽  
Novel Method ◽  
Sec System

Abstract A robust and reproducible method for the molar mass analysis of neutral and anionic copolymers based on methacrylic acid and different (meth)acrylates has been developed. Size exclusion chromatography (SEC) using a novel polyester- based packing as the stationary phase and dimethylacetamide (DMAC) as the mobile phase yields highly accurate results for copolymers with a methacrylic acid content up to 50 wt.-%. To suppress the different polar and ionic interactions between the sample molecules, the stationary phase and the eluent, DMAC was modified with LiBr and acetic acid. Calibrating the SEC system with poly(methyl methacrylate) of narrow polydispersity, molar masses were obtained that are in good agreement with data obtained by SEC-MALLS (multi-angle laser light scattering) and SEC measurements of methylated samples. The reproducibility and the robustness of the novel method were proven by running similar samples in three different laboratories and for an extended period of two weeks.

Sign in / Sign up

Export Citation Format

Share Document