Adaptive, Model-Based Control by the Open-Loop-Feedback-Optimal (OLFO) Controller for the Effective Fed-Batch Cultivation of Hybridoma Cells

2002 ◽  
Vol 18 (5) ◽  
pp. 1095-1103 ◽  
Author(s):  
B. Frahm ◽  
P. Lane ◽  
H. Atzert ◽  
A. Munack ◽  
M. Hoffmann ◽  
...  
Keyword(s):  
Batch Cultivation ◽  
Open Loop ◽  
Hybridoma Cells ◽  
Adaptive Model ◽  
Fed Batch ◽  
Model Based Control ◽  
Model Based ◽  
Loop Feedback ◽  
Fed Batch Cultivation

Modeling and Model Based Feeding Control for Pichia pastoris Fed-Batch Cultivation

2004 ◽  
Vol 37 (1) ◽  
pp. 451-456
Author(s):  
H.T. Ren ◽  
J.H. Deng ◽  
B.K. He ◽  
L.M. Ren ◽  
J.Q. Yuan
Keyword(s):  
Pichia Pastoris ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Model Based ◽  
Feeding Control ◽  
Fed Batch Cultivation

Development of an Automated, Adaptive Model-Based Control for the Effective Cultivation of Hybridoma Cells

2001 ◽  
pp. 446-448
Author(s):  
Bjoern Frahm ◽  
H. Atzert ◽  
P. Lane ◽  
M. Hoffmann ◽  
V. C. Hass ◽  
...  
Keyword(s):  
Hybridoma Cells ◽  
Adaptive Model ◽  
Model Based Control ◽  
Model Based

scholarly journals Optimization of Synthetic Media Composition for Kluyveromyces marxianus Fed-Batch Cultivation

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 62
Author(s):  
Konstantins Dubencovs ◽  
Janis Liepins ◽  
Arturs Suleiko ◽  
Anastasija Suleiko ◽  
Reinis Vangravs ◽  
...  
Keyword(s):  
Kluyveromyces Marxianus ◽  
Reaction Medium ◽  
Value Added ◽  
Batch Cultivation ◽  
Batch Processes ◽  
Biomass Growth ◽  
High Cell ◽  
Media Composition ◽  
Fed Batch ◽  
Fed Batch Cultivation

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order to intensify the biosynthesis rate of a target product, reaching high biomass concentrations in the reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how to achieve high cell densities. However, depending on the physiology of the particular microbial strain, an optimal media composition should be used to avoid by-product synthesis and, subsequently, a decrease in overall process effi-ciency. Thus, the aim of the present study was to optimise the synthetic growth medium and feeding solution compositions (in terms of carbon, nitrogen, phosphorous, magnesium, and calcium concentrations) for high cell density K. marxianus fed‑batch cultivations. Additionally, the biomass yields from the vitamin mixture and other macro/microelements were identified. A model predictive control algorithm was successfully applied for a fed-batch cultivation control. Biomass growth and substrate consumption kinetics were compared with the mathematical model predictions. Finally, 2‑phenylethanol biosynthesis was induced and its productivity was estimated. The determined optimal macronutrient ratio for K. marxianus biomass growth was identified as C:N:P = 1:0.07:0.011. The maximal attained yeast biomass concentration was close to 70 g·L-1 and the 2-PE biosynthesis rate was 0.372 g·L−1·h−1, with a yield of 74% from 2-phenylalanine.

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