Field-dependent aluminum-27 NMR studies of the transferrins: an approach for the study of metal ion binding sites in larger proteins

1993 ◽  
Vol 115 (21) ◽  
pp. 9750-9753 ◽  
Author(s):  
James M. Aramini ◽  
Markus W. Germann ◽  
Hans J. Vogel
Keyword(s):  
Binding Sites ◽  
Metal Ion ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Nmr Studies ◽  
Field Dependent ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

Quadrupolar metal ion NMR studies of metalloproteins

1998 ◽  
Vol 76 (2-3) ◽  
pp. 210-222 ◽  
Author(s):  
James M Aramini ◽  
Hans J Vogel
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Metal Ion ◽  
Chemical Information ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Nmr Studies ◽  
Small Proteins ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

We present a summary of the quadrupolar metal ion NMR studies of metalloproteins conducted in our laboratory in recent years. The approaches we employ can be subdivided into two categories: (i) the use of low-frequency metal nuclei to probe metal ion binding sites in small proteins, exemplified by 43Ca NMR studies of alpha-lactalbumins and calcium-binding lysozymes, and (ii) the novel detection of the central transition of half-integer quadrupolar nuclei of moderate frequency bound to large metalloproteins, typified by 27Al, 45Sc, 69,71Ga, and 51V NMR studies of the transferrins. We highlight the chemical information regarding the nature of metal ion binding sites that can be obtained from this technique and emphasize the salient parameters that an investigator must consider to successfully apply quadrupolar NMR to the study of biological macromolecules.Key words: quadrupolar NMR, metalloproteins, transferrins.

scholarly journals Specific metal-ion binding sites in a model of the P4-P6 triple-helical domain of a group I intron

RNA ◽  
2001 ◽  
Vol 7 (8) ◽  
pp. 1115-1125 ◽  
Author(s):  
MARTINA LINDQVIST ◽  
KARIN SANDSTRÖM ◽  
VILNIS LIEPINS ◽  
ROGER STRÖMBERG ◽  
ASTRID GRÄSLUND
Keyword(s):  
Binding Sites ◽  
Metal Ion ◽  
Group I Intron ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Group I ◽  
Ion Binding Sites ◽  
Triple Helical Domain ◽  
Metal Ion Binding Sites ◽  
Specific Metal

Rhodopsin activation blocked by metal-ion-binding sites linking transmembrane helices C and F

Nature ◽  
1996 ◽  
Vol 383 (6598) ◽  
pp. 347-350 ◽  
Author(s):  
Søren P. Sheikh ◽  
Tatyana A. Zvyaga ◽  
Olivier Lichtarge ◽  
Thomas P. Sakmar ◽  
Henry R. Bourne
Keyword(s):  
Binding Sites ◽  
Metal Ion ◽  
Ion Binding ◽  
Transmembrane Helices ◽  
Metal Ion Binding ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

Multinuclear magnetic resonance studies of metal ion binding sites of phosphoglucomutase

Biochemistry ◽  
1985 ◽  
Vol 24 (10) ◽  
pp. 2536-2541 ◽  
Author(s):  
Gyung Ihm Rhyu ◽  
William J. Ray ◽  
John L. Markley
Keyword(s):  
Magnetic Resonance ◽  
Binding Sites ◽  
Metal Ion ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Magnetic Resonance Studies ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites ◽  
Multinuclear Magnetic Resonance

Lanthanide ion luminescence probes. Characterization of metal ion binding sites and intermetal energy transfer distance measurements in calcium-binding proteins. 2. Thermolysin

Biochemistry ◽  
1981 ◽  
Vol 20 (12) ◽  
pp. 3334-3339 ◽  
Author(s):  
A. Peter Snyder ◽  
Daniel R. Sudnick ◽  
Valerie K. Arkle ◽  
William D. Horrocks
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Metal Ion ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Distance Measurements ◽  
Lanthanide Ion ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

scholarly journals Identification of metal ion binding sites based on amino acid sequences

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0183756 ◽  
Author(s):  
Xiaoyong Cao ◽  
Xiuzhen Hu ◽  
Xiaojin Zhang ◽  
Sujuan Gao ◽  
Changjiang Ding ◽  
...  
Keyword(s):  
Amino Acid ◽  
Binding Sites ◽  
Metal Ion ◽  
Amino Acid Sequences ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites
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