scholarly journals Structural Studies of the Molybdenum Center of the Pathogenic R160Q Mutant of Human Sulfite Oxidase by Pulsed EPR Spectroscopy and17O and33S Labeling

2008 ◽  
Vol 130 (26) ◽  
pp. 8471-8480 ◽  
Author(s):  
Andrei V. Astashkin ◽  
Kayunta Johnson-Winters ◽  
Eric L. Klein ◽  
Changjian Feng ◽  
Heather L. Wilson ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Sulfite Oxidase ◽  
Structural Studies ◽  
Pulsed Epr ◽  
Human Sulfite Oxidase

scholarly journals Determination of the Distance between the Mo(V) and Fe(III) Heme Centers of Wild Type Human Sulfite Oxidase by Pulsed EPR Spectroscopy

2012 ◽  
Vol 116 (6) ◽  
pp. 1942-1950 ◽  
Author(s):  
Andrei V. Astashkin ◽  
Asha Rajapakshe ◽  
Matthew J. Cornelison ◽  
Kayunta Johnson-Winters ◽  
John H. Enemark
Keyword(s):  
Epr Spectroscopy ◽  
Sulfite Oxidase ◽  
Wild Type ◽  
Pulsed Epr ◽  
Human Sulfite Oxidase

scholarly journals Studies of the Mo(V) center of the Y343F mutant of human sulfite oxidase by variable frequency pulsed EPR spectroscopy

2008 ◽  
Vol 361 (4) ◽  
pp. 941-946 ◽  
Author(s):  
Arnold M. Raitsimring ◽  
Andrei V. Astashkin ◽  
Changjian Feng ◽  
Heather L. Wilson ◽  
K.V. Rajagopalan ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Sulfite Oxidase ◽  
Variable Frequency ◽  
Pulsed Epr ◽  
Human Sulfite Oxidase

scholarly journals Structural Studies of Membrane Proteins using Pulsed EPR Spectroscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 56a
Author(s):  
Gary A. Lorigan ◽  
Indra D. Sahu ◽  
Daniel L. Drew ◽  
Gunjan Dixit ◽  
Tanbir Ahammad
Keyword(s):  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Structural Studies ◽  
Pulsed Epr

scholarly journals Applications of pulsed EPR spectroscopy to structural studies of sulfite oxidizing enzymes

2013 ◽  
Vol 257 (1) ◽  
pp. 110-118 ◽  
Author(s):  
Eric L. Klein ◽  
Andrei V. Astashkin ◽  
Arnold M. Raitsimring ◽  
John H. Enemark
Keyword(s):  
Epr Spectroscopy ◽  
Structural Studies ◽  
Pulsed Epr

scholarly journals Consensus structures of the Mo(v) sites of sulfite-oxidizing enzymes derived from variable frequency pulsed EPR spectroscopy, isotopic labelling and DFT calculations

2017 ◽  
Vol 46 (39) ◽  
pp. 13202-13210 ◽  
Author(s):  
John H. Enemark
Keyword(s):  
Dft Calculations ◽  
Epr Spectroscopy ◽  
Sulfite Oxidase ◽  
Variable Frequency ◽  
Isotopic Labelling ◽  
Pulsed Epr

The “blocked” form of sulfite oxidase has O-bound sulfite, and only the coordinated and remote O atoms exchange with H217O.

Structures of the Mo(V) Forms of Sulfite Oxidase fromArabidopsis thalianaby Pulsed EPR Spectroscopy†

Biochemistry ◽  
2005 ◽  
Vol 44 (40) ◽  
pp. 13274-13281 ◽  
Author(s):  
Andrei V. Astashkin ◽  
Brian L. Hood ◽  
Changjian Feng ◽  
Russ Hille ◽  
Ralf R. Mendel ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Sulfite Oxidase ◽  
Pulsed Epr

scholarly journals Structural Studies of the Molybdenum Center of Mitochondrial Amidoxime Reducing Component (mARC) by Pulsed EPR Spectroscopy and17O-Labeling

Biochemistry ◽  
2011 ◽  
Vol 50 (41) ◽  
pp. 8813-8822 ◽  
Author(s):  
Asha Rajapakshe ◽  
Andrei V. Astashkin ◽  
Eric L. Klein ◽  
Debora Reichmann ◽  
Ralf R. Mendel ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Structural Studies ◽  
Pulsed Epr

scholarly journals Structures and reaction pathways of the molybdenum centres of sulfite-oxidizing enzymes by pulsed EPR spectroscopy

2008 ◽  
Vol 36 (6) ◽  
pp. 1129-1133 ◽  
Author(s):  
John H. Enemark ◽  
Andrei V. Astashkin ◽  
Arnold M. Raitsimring
Keyword(s):  
Epr Spectroscopy ◽  
Reaction Pathways ◽  
Oxidase Deficiency ◽  
Pulsed Epr ◽  
Product Release ◽  
Nuclear Quadrupole Interactions ◽  
Sulfite Oxidase Deficiency ◽  
Nuclear Quadrupole ◽  
Human Sulfite Oxidase ◽  
Insight Into

SOEs (sulfite-oxidizing enzymes) are physiologically vital and occur in all forms of life. During the catalytic cycle, the five-co-ordinate square pyramidal oxo-molybdenum active site passes through the Mo(V) state, and intimate details of the structure can be obtained from variable frequency pulsed EPR spectroscopy through the hyperfine and nuclear quadrupole interactions of nearby magnetic nuclei. By employing variable spectrometer operational frequencies, it is possible to optimize the measurement conditions for difficult quadrupolar nuclei of interest (e.g. 17O, 33S, 35Cl and 37Cl) and to simplify the interpretation of the spectra. Isotopically labelled model Mo(V) compounds provide further insight into the electronic and geometric structures and chemical reactions of the enzymes. Recently, blocked forms of SOEs having co-ordinated sulfate, the reaction product, were detected using 33S (I=3/2) labelling. This blocking of product release is a possible contributor to fatal human sulfite oxidase deficiency in young children.

scholarly journals Aminoxyl Radicals of B/P Frustrated Lewis Pairs: Refinement of the Spin-Hamiltonian Parameters by Field- and Temperature-Dependent Pulsed EPR Spectroscopy

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157944 ◽  
Author(s):  
Marcos de Oliveira ◽  
Robert Knitsch ◽  
Muhammad Sajid ◽  
Annika Stute ◽  
Lisa-Maria Elmer ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Spin Hamiltonian ◽  
Frustrated Lewis Pairs ◽  
Temperature Dependent ◽  
Pulsed Epr ◽  
Hamiltonian Parameters ◽  
Aminoxyl Radicals ◽  
Lewis Pairs
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