Selective Covalent Labeling of Tag-Fused GPCR Proteins on Live Cell Surface with a Synthetic Probe for Their Functional Analysis

2010 ◽  
Vol 132 (27) ◽  
pp. 9301-9309 ◽  
Author(s):  
Hiroshi Nonaka ◽  
Sho-hei Fujishima ◽  
Sho-hei Uchinomiya ◽  
Akio Ojida ◽  
Itaru Hamachi
Keyword(s):  
Functional Analysis ◽  
Cell Surface ◽  
Live Cell ◽  
Covalent Labeling ◽  
Synthetic Probe

scholarly journals Cell Surface Receptors: Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow-Based Microfluidic Device (Small 8/2015)

Small ◽  
2015 ◽  
Vol 11 (8) ◽  
pp. 1012-1012
Author(s):  
Ramesh Ramji ◽  
Cheong Fook Cheong ◽  
Hiroaki Hirata ◽  
Abdur Rub Abdur Rahman ◽  
Chwee Teck Lim
Keyword(s):  
Cell Surface ◽  
Microfluidic Device ◽  
Live Cell ◽  
Cell Surface Receptors ◽  
Cell Receptors ◽  
Surface Receptors ◽  
Rapid Quantification

Generation of Live-Cell Microarrays by Means of DNA-Directed Immobilization of Specific Cell-Surface Ligands

2007 ◽  
Vol 46 (22) ◽  
pp. 4180-4183 ◽  
Author(s):  
Hendrik Schroeder ◽  
Bernhard Ellinger ◽  
Christian F. W. Becker ◽  
Herbert Waldmann ◽  
Christof M. Niemeyer
Keyword(s):  
Cell Surface ◽  
Live Cell ◽  
Specific Cell ◽  
Surface Ligands ◽  
Cell Microarrays ◽  
Specific Cell Surface

Functional Analysis of Monocyte-Macrophages Derived From Nonadherent Cord Blood Progenitor Cells: Correlation With the Ontogeny of Cell Surface Proteins

1989 ◽  
Vol 46 (3) ◽  
pp. 230-238 ◽  
Author(s):  
Frances Santiago-Schwarz ◽  
Donna M. McHugh ◽  
Howard B. Fleit
Keyword(s):  
Functional Analysis ◽  
Cell Surface ◽  
Cord Blood ◽  
Progenitor Cells ◽  
Surface Proteins ◽  
Cell Surface Proteins

scholarly journals Domain and Functional Analysis of a Novel Platelet-Endothelial Cell Surface Protein, SCUBE1

2008 ◽  
Vol 283 (18) ◽  
pp. 12478-12488 ◽  
Author(s):  
Cheng-Fen Tu ◽  
Yu-Ting Yan ◽  
Szu-Yao Wu ◽  
Bambang Djoko ◽  
Ming-Tzu Tsai ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Endothelial Cell ◽  
Cell Surface ◽  
Surface Protein ◽  
Cell Surface Protein ◽  
Endothelial Cell Surface

scholarly journals Use of Novel Mutant Galactosyltransferase for the Bioconjugation of Terminal N-Acetylglucosamine (GlcNAc) Residues on Live Cell Surface

2013 ◽  
Vol 24 (1) ◽  
pp. 144-152 ◽  
Author(s):  
Natalia Mercer ◽  
Boopathy Ramakrishnan ◽  
Elizabeth Boeggeman ◽  
Luke Verdi ◽  
Pradman K. Qasba
Keyword(s):  
Cell Surface ◽  
Live Cell

scholarly journals Golgi, trafficking, and mitosis dysfunctions in pulmonary arterial endothelial cells exposed to monocrotaline pyrrole and NO scavenging

2009 ◽  
Vol 297 (4) ◽  
pp. L715-L728 ◽  
Author(s):  
Jason Lee ◽  
Reuben Reich ◽  
Fang Xu ◽  
Pravin B. Sehgal
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Cell Surface ◽  
Live Cell ◽  
Cell Uptake ◽  
Important Distinction ◽  
No Donor ◽  
Pulmonary Arterial ◽  
Monocrotaline Pyrrole ◽  
Arterial Endothelial Cells

Although the administration of monocrotaline (MCT) into experimental animals is in widespread use today in investigations of pulmonary arterial hypertension (PAH), the underlying cellular and subcellular mechanisms that culminate in vascular remodeling are incompletely understood. Bovine pulmonary arterial endothelial cells (PAECs) in culture exposed to monocrotaline pyrrole (MCTP) develop “megalocytosis” 18–24 h later characterized by enlarged hyperploid cells with enlarged Golgi, mislocalization of endothelial nitric oxide synthase away from the plasma membrane, decreased cell-surface/caveolar nitric oxide (NO), and hypo- S-nitrosylation of caveolin-1, clathrin heavy chain, and N-ethylmaleimide-sensitive factor. We investigated whether MCTP did in fact affect functional intracellular trafficking. The NO scavenger (4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) and the NO donor diethylamine NONOate were used for comparison. Both MCTP and c-PTIO produced distinctive four- to fivefold enlarged PAECs within 24–48 h with markedly enlarged/dispersed Golgi, as visualized by immunostaining for the Golgi tethers/matrix proteins giantin, GM130, and p115. Live-cell uptake of the Golgi marker C5 ceramide revealed a compact juxtanuclear Golgi in untreated PAECs, brightly labeled enlarged circumnuclear Golgi after MCTP, but minimally labeled Golgi elements after c-PTIO. These Golgi changes were reduced by NONOate. After an initial inhibition during the first day, both MCTP and c-PTIO markedly enhanced anterograde secretion of soluble cargo (exogenous vector-expressed recombinant horseradish peroxidase) over the next 4 days. Live-cell internalization assays using fluorescently tagged ligands showed that both MCTP and c-PTIO inhibited the retrograde uptake of acetylated low-density lipoprotein, transferrin, and cholera toxin B. Moreover, MCTP, and to a variable extent c-PTIO, reduced the cell-surface density of all receptors assayed (LDLR, TfnR, BMPR, Tie-2, and PECAM-1/CD31). In an important distinction, c-PTIO enhanced mitosis in PAECs but MCTP inhibited mitosis, even that due to c-PTIO, despite markedly exaggerated Golgi dispersal. Taken together, these data define a broad-spectrum Golgi and subcellular trafficking dysfunction syndrome in endothelial cells exposed to MCTP or NO scavenging.

scholarly journals Live Cell Surface Conjugation Methods for Imaging, Sensing and Therapy

2018 ◽  
Vol 114 (3) ◽  
pp. 20a ◽  
Author(s):  
Joydeb Majumder ◽  
Gaurav Chopra
Keyword(s):  
Cell Surface ◽  
Live Cell

Analysis of Cell-Surface Receptor Dynamics through Covalent Labeling by Catalyst-Tethered Antibody

2015 ◽  
Vol 137 (16) ◽  
pp. 5372-5380 ◽  
Author(s):  
Takahiro Hayashi ◽  
Yuki Yasueda ◽  
Tomonori Tamura ◽  
Yousuke Takaoka ◽  
Itaru Hamachi
Keyword(s):  
Cell Surface ◽  
Cell Surface Receptor ◽  
Covalent Labeling ◽  
Receptor Dynamics ◽  
Surface Receptor

scholarly journals Nano-Scale Topography Imaging of Live Cell Surface Using Scanning Ion Conductance Microscopy

Hyomen Kagaku ◽  
2015 ◽  
Vol 36 (6) ◽  
pp. 313-318 ◽  
Author(s):  
Hiroki IDA ◽  
Yasufumi TAKAHASHI ◽  
Hitoshi SHIKU ◽  
Tomokazu MATSUE
Keyword(s):  
Cell Surface ◽  
Live Cell ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Nano Scale
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