Solution NMR Study of the Electronic and Molecular Structure of the Heme Cavity in High-Spin, Resting State Horseradish Peroxidase

1997 ◽  
Vol 119 (20) ◽  
pp. 4732-4739 ◽  
Author(s):  
Jeffrey S. de Ropp ◽  
Pravat Mandal ◽  
Samuel L. Brauer ◽  
Gerd N. La Mar
Keyword(s):  
Molecular Structure ◽  
Horseradish Peroxidase ◽  
High Spin ◽  
Resting State ◽  
Solution Nmr ◽  
Nmr Study

Expression and Solution NMR Study of Multi-site Phosphomimetic Mutant BCL-2 Protein

2019 ◽  
Vol 26 (6) ◽  
pp. 449-457
Author(s):  
Ting Song ◽  
Keke Cao ◽  
Yu dan Fan ◽  
Zhichao Zhang ◽  
Zong W. Guo ◽  
...  
Keyword(s):  
Structural Change ◽  
Structural Biology ◽  
Quantum Coherence ◽  
Structural Changes ◽  
Solution Nmr ◽  
Flexible Loop ◽  
Loop Region ◽  
Loop Domain ◽  
Nmr Study ◽  
Binding Groove

Background: The significance of multi-site phosphorylation of BCL-2 protein in the flexible loop domain remains controversial, in part due to the lack of structural biology studies of phosphorylated BCL-2. Objective: The purpose of the study is to explore the phosphorylation induced structural changes of BCL-2 protein. Methods: We constructed a phosphomietic mutant BCL-2(62-206) (t69e, s70e and s87e) (EEEBCL- 2-EK (62-206)), in which the BH4 domain and the part of loop region was truncated (residues 2-61) to enable a backbone resonance assignment. The phosphorylation-induced structural change was visualized by overlapping a well dispersed 15N-1H heteronuclear single quantum coherence (HSQC) NMR spectroscopy between EEE-BCL-2-EK (62-206) and BCL-2. Results: The EEE-BCL-2-EK (62-206) protein reproduced the biochemical and cellular activity of the native phosphorylated BCL-2 (pBCL-2), which was distinct from non-phosphorylated BCL-2 (npBCL-2) protein. Some residues in BH3 binding groove occurred chemical shift in the EEEBCL- 2-EK (62-206) spectrum, indicating that the phosphorylation in the loop region induces a structural change of active site. Conclusion: The phosphorylation of BCL-2 induced structural change in BH3 binding groove.

scholarly journals Electronic and Molecular Structure of High-Spin d4Complexes:  Experimental and Theoretical Study of the [Cr(D2O)6]2+Cation in Tutton's Salts [J. Am. Chem. Soc.2004,126, 16639−16652].

2005 ◽  
Vol 127 (10) ◽  
pp. 3642-3642 ◽  
Author(s):  
Christopher Dobe ◽  
Christopher Noble ◽  
Graham Carver ◽  
Philip L. W. Tregenna-Piggott ◽  
Garry J. McIntyre ◽  
...  
Keyword(s):  
Molecular Structure ◽  
High Spin ◽  
Theoretical Study ◽  
Tutton’S Salts

Solution NMR Study of Liquid Silk

2016 ◽  
Vol 72 (4) ◽  
pp. P-208-P-208
Author(s):  
YU SUZUKI
Keyword(s):  
Solution Nmr ◽  
Nmr Study

Combined X-ray Diffraction and15N CPMAS NMR Study of Molecular Structure and Proton Order/Disorder Phenomena in CyclicN,N‘-Bisarylformamidine Dimers

1997 ◽  
Vol 119 (50) ◽  
pp. 12223-12230 ◽  
Author(s):  
Romana Anulewicz ◽  
Iwona Wawer ◽  
Tadeusz Marek Krygowski ◽  
Ferdinand Männle ◽  
Hans-Heinrich Limbach
Keyword(s):  
Molecular Structure ◽  
X Ray Diffraction ◽  
X Ray ◽  
Nmr Study

Proton NMR study of high-spin ferric natural porphyrin derivatives as models of methemoproteins

1979 ◽  
Vol 101 (20) ◽  
pp. 6091-6096 ◽  
Author(s):  
David L. Budd ◽  
Gerd N. La Mar ◽  
Kevin C. Langry ◽  
Kevin M. Smith ◽  
R. Nayyir-Mazhir
Keyword(s):  
High Spin ◽  
Proton Nmr ◽  
Porphyrin Derivatives ◽  
Nmr Study
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