scholarly journals Direct in Vitro Selection of Trans-Acting Ribozymes for Posttranscriptional, Site-Specific, and Covalent Fluorescent Labeling of RNA

2019 ◽  
Vol 141 (50) ◽  
pp. 19546-19549 ◽  
Author(s):  
Mohammad Ghaem Maghami ◽  
Carolin P. M. Scheitl ◽  
Claudia Höbartner
Keyword(s):  
In Vitro Selection ◽  
Fluorescent Labeling ◽  
Site Specific ◽  
Selection Of

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups

2000 ◽  
Vol 15 (4) ◽  
pp. 297-308 ◽  
Author(s):  
NAOZUMI TERAMOTO ◽  
YUKIO IMANISHI ◽  
YOSHIHIRO ITO
Keyword(s):  
In Vitro Selection ◽  
Amino Groups ◽  
Selection Of

scholarly journals Back Cover: In Vitro Selection of a DNA Aptamer Targeting Degraded Protein Fragments for Biosensing (Angew. Chem. Int. Ed. 20/2020)

2020 ◽  
Vol 59 (20) ◽  
pp. 7968-7968
Author(s):  
Meng Liu ◽  
Jiayi Wang ◽  
Yangyang Chang ◽  
Qiang Zhang ◽  
Dingran Chang ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Dna Aptamer ◽  
Protein Fragments ◽  
Back Cover ◽  
Selection Of

In vitro selection of a fungal pathogen for use against Diaphorina citri

2015 ◽  
Vol 90 ◽  
pp. 6-15 ◽  
Author(s):  
N. Orduño-Cruz ◽  
A.W. Guzmán-Franco ◽  
E. Rodríguez-Leyva ◽  
R. Alatorre-Rosas ◽  
H. González-Hernández ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
In Vitro Selection ◽  
Diaphorina Citri ◽  
Selection Of
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