scholarly journals Mass Spectrometry Imaging of Arabidopsis thaliana Leaves at the Single-Cell Level by Infrared Laser Ablation Atmospheric Pressure Photoionization (LAAPPI)

2021 ◽  
Author(s):  
Juha-Pekka Hieta ◽  
Nina Sipari ◽  
Heikki Räikkönen ◽  
Markku Keinänen ◽  
Risto Kostiainen
Keyword(s):  
Mass Spectrometry ◽  
Arabidopsis Thaliana ◽  
Laser Ablation ◽  
Single Cell ◽  
Atmospheric Pressure ◽  
Mass Spectrometry Imaging ◽  
Infrared Laser ◽  
Single Cell Level ◽  
Cell Level ◽  
Atmospheric Pressure Photoionization

Laser ablation atmospheric pressure photoionization mass spectrometry imaging of phytochemicals from sage leaves

2014 ◽  
Vol 28 (23) ◽  
pp. 2490-2496 ◽  
Author(s):  
Anu Vaikkinen ◽  
Bindesh Shrestha ◽  
Juha Koivisto ◽  
Risto Kostiainen ◽  
Akos Vertes ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Atmospheric Pressure ◽  
Mass Spectrometry Imaging ◽  
Atmospheric Pressure Photoionization ◽  
Photoionization Mass Spectrometry

Infrared Laser Ablation Atmospheric Pressure Photoionization Mass Spectrometry

2012 ◽  
Vol 84 (3) ◽  
pp. 1630-1636 ◽  
Author(s):  
Anu Vaikkinen ◽  
Bindesh Shrestha ◽  
Tiina J. Kauppila ◽  
Akos Vertes ◽  
Risto Kostiainen
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Atmospheric Pressure ◽  
Infrared Laser ◽  
Atmospheric Pressure Photoionization ◽  
Photoionization Mass Spectrometry

Laser Ablation-Inductively Coupled Plasma Time-of-Flight Mass Spectrometry Imaging of Trace Elements at the Single-Cell Level for Clinical Practice

2019 ◽  
Vol 91 (13) ◽  
pp. 8207-8212 ◽  
Author(s):  
Sarah Theiner ◽  
Andreas Schweikert ◽  
Stijn J. M. Van Malderen ◽  
Anna Schoeberl ◽  
Sophie Neumayer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Trace Elements ◽  
Clinical Practice ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Mass Spectrometry Imaging ◽  
Single Cell Level ◽  
Cell Level ◽  
Inductively Coupled ◽  
Flight Mass Spectrometry

Infrared Laser-Mediated Gene Induction at the Single-Cell Level in the Regenerating Tail of Xenopus laevis Tadpoles

2018 ◽  
Vol 2018 (12) ◽  
pp. pdb.prot101014 ◽  
Author(s):  
Riho Hasugata ◽  
Shinichi Hayashi ◽  
Aiko Kawasumi-Kita ◽  
Joe Sakamoto ◽  
Yasuhiro Kamei ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Single Cell ◽  
Gene Induction ◽  
Infrared Laser ◽  
Single Cell Level ◽  
Cell Level

Gene expression profiling of the different stages of Arabidopsis thaliana trichome development on the single cell level

2008 ◽  
Vol 46 (2) ◽  
pp. 160-173 ◽  
Author(s):  
Sergiy Kryvych ◽  
Victoria Nikiforova ◽  
Michel Herzog ◽  
Daniel Perazza ◽  
Joachim Fisahn
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Single Cell ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Single Cell Level ◽  
Cell Level ◽  
Trichome Development

Ambient Mass Spectrometry Imaging with Picosecond Infrared Laser Ablation Electrospray Ionization (PIR-LAESI)

2015 ◽  
Vol 87 (24) ◽  
pp. 12071-12079 ◽  
Author(s):  
Jing Zou ◽  
Francis Talbot ◽  
Alessandra Tata ◽  
Leonardo Ermini ◽  
Kresimir Franjic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Infrared Laser ◽  
Ambient Mass Spectrometry ◽  
Laser Ablation Electrospray Ionization

scholarly journals Laser cleavable probes for in situ multiplexed glycan detection by single cell mass spectrometry

2019 ◽  
Vol 10 (47) ◽  
pp. 10958-10962 ◽  
Author(s):  
Jing Han ◽  
Xi Huang ◽  
Huihui Liu ◽  
Jiyun Wang ◽  
Caiqiao Xiong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Resistance ◽  
Single Cell ◽  
Cell Mass ◽  
Ionization Efficiency ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Mass Spectrometry ◽  
The Relationship

A single-cell MS approach for multiplexed glycan detection to investigate the relationship between drug resistance and glycans at a single-cell level and quantify multiple glycans, overcoming the limit of low ionization efficiency of glycans.

scholarly journals Ultra-streamlined single cell proteomics by all-in-one chip and data-independent-acquisition mass spectrometry

2021 ◽  
Author(s):  
Hsiung-Lin Tu ◽  
Sofani Gebreyesus ◽  
Asad Ali Siyal ◽  
Reta Birhanu Kitata ◽  
Eric Sheng-Wen Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Missing Values ◽  
Protein Quantification ◽  
Single Cell Level ◽  
Cell Level ◽  
Proteome Coverage ◽  
Data Independent Acquisition ◽  
General Utility ◽  
Ultrahigh Sensitivity

Abstract Single cell proteomics provides the ultimate resolution to reveal cellular phenotypic heterogeneity and functional network underlying biological processes. Here, we present an ultra-streamlined workflow combining an integrated proteomic chip (iProChip) and data-independent-acquisition (DIA) mass spectrometry for sensitive microproteomics analysis down to single cell level. The iProChip offers multiplexed and automated all-in-one station from cell isolation/counting/imaging to complete proteomic processing within a single device. By mapping to project-specific spectra libraries, the iProChip-DIA enables profiling of 1160 protein groups from triplicate analysis of a single mammalian cell. Furthermore, the applicability of iProChip-DIA was demonstrated using both adherent and non-adherent malignant cells, which reveals 5 orders of proteome coverage, highly consistent ~100-fold protein quantification (1-100 cells) and high reproducibility with low missing values (<16%). With the demonstrated all-in-one cell characterization, ultrahigh sensitivity, robustness, and versatility to add other functionalities, the iProChip-DIA is anticipated to offer general utility to realize advanced proteomics applications at single cell level.

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